PMID- 32828006
OWN - NLM
STAT- MEDLINE
DCOM- 20210810
LR  - 20210810
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 100
DP  - 2020 Oct
TI  - Placental distribution of endogenous and exogenous substances: A pilot study 
      utilizing cryo-sampled specimen off delivery room.
PG  - 45-53
LID - S0143-4004(20)30262-9 [pii]
LID - 10.1016/j.placenta.2020.08.009 [doi]
AB  - INTRODUCTION: Reliability in the use of placentome (including placenta, umbilical 
      cord, and cord blood) biomarkers requires an understanding of their 
      distributions. Here we aim to develop a simple and proper placenta sampling 
      scheme, and to evaluate the placental distributions of biomarkers. METHODS: We 
      developed a continuous cooling chain protocol off delivery room and 
      cryo-subsampling method for placenta sampling. The levels of thyroid hormones 
      (THs), elements, persistent organic pollutants (POPs), monoamines, and vitamin E 
      were measured using UPLC-Q-TOF-MS, HPLC-ICP-MS, HPLC-EcD, and HRGC-HRMS, 
      respectively. The distributions of biomarkers were assessed. RESULTS: In human 
      placentome, (l)-thyroxine (T(4)), Cd, Se, Zn, Cu, Fe, Ca, K, Mg, alpha-tocopherol, 
      beta-tocopherol, and beta-tocotrienol levels were higher in placenta than in umbilical 
      cord, while Pb and Mn were concentrated in human cord. In porcine placentome, 
      T(4), 3,3',5'-triiodo-(l)-thyronine (rT(3)), 3,3'-diiodo-(l)-thyronine, Cd, Pb, 
      Zn, K, and Al levels were higher in the cord. The intraclass correlation 
      coefficient (ICC) was <0.4 for 3,3',5-triiodo-(l)-thyronine, rT(3), alpha-tocopherol, 
      and 7 elements in human basal plate, indicating low reliability. rT(3), Cd, Zn, 
      Mn, and Cu were significantly concentrated in the central region in human 
      placenta, while higher levels of As, Cd, Cr, and Al were found in the periphery 
      region in porcine placenta. Polychlorinated biphenyls (PCBs) and polybrominated 
      diphenyl ethers (PBDEs) showed moderate reliability (ICC: 0.40-0.98) except 
      PCB-81, -126, and BDE-208, while polychlorinated dibenzo-p-doixins/furans 
      (PCDD/Fs) showed poor reliability (ICC: 0.07-0.31). DISCUSSION: These results 
      highlight the complexity of placenta sampling. This study provides a novel and 
      simple sampling approach in investigating placental exposomics.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Li, Zhong-Min
AU  - Li ZM
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Molecular EXposomics, Ingolstadter Landstr. 1, 85764, Neuherberg, Germany; School 
      of Life Sciences Weihenstephan (Nutrition), Technische Universitat Munchen, 
      85354, Freising, Germany. Electronic address: zhong-min.li@helmholtz-muenchen.de.
FAU - Benker, Barbel
AU  - Benker B
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Research Unit Analytical BioGeoChemistry, Ingolstadter Landstr. 1, 85764, 
      Neuherberg, Germany.
FAU - Bao, Qibei
AU  - Bao Q
AD  - Ningbo College of Health Sciences, 315100, Ningbo, Zhejiang, China.
FAU - Henkelmann, Bernhard
AU  - Henkelmann B
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Molecular EXposomics, Ingolstadter Landstr. 1, 85764, Neuherberg, Germany.
FAU - Corsten, Claudia
AU  - Corsten C
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Molecular EXposomics, Ingolstadter Landstr. 1, 85764, Neuherberg, Germany.
FAU - Michalke, Bernhard
AU  - Michalke B
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Research Unit Analytical BioGeoChemistry, Ingolstadter Landstr. 1, 85764, 
      Neuherberg, Germany.
FAU - Pauluschke-Frohlich, Jan
AU  - Pauluschke-Frohlich J
AD  - Department fur Frauengesundheit Universitats-Frauenklinik Tubingen, Calwerstr. 7, 
      70276, Tubingen, Germany.
FAU - Flisikowski, Krzysztof
AU  - Flisikowski K
AD  - Lehrstuhl fur Biotechnologie der Nutztiere, Technische Universitat Munchen, 
      Liesel-Beckmannstr. 1, 85354, Freising, Germany.
FAU - Schramm, Karl-Werner
AU  - Schramm KW
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Molecular EXposomics, Ingolstadter Landstr. 1, 85764, Neuherberg, Germany; 
      Department fur Biowissenschaftliche Grundlagen, Technische Universitat Munchen, 
      Weihenstephaner Steig 23, 85350, Freising, Germany.
FAU - De Angelis, Meri
AU  - De Angelis M
AD  - Helmholtz Zentrum Munchen-German Research Center for Environmental Health (GmbH), 
      Molecular EXposomics, Ingolstadter Landstr. 1, 85764, Neuherberg, Germany. 
      Electronic address: meri.deangelis@helmholtz-muenchen.de.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200811
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Biogenic Monoamines)
RN  - 0 (Thyroid Hormones)
RN  - 1406-18-4 (Vitamin E)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Biogenic Monoamines/analysis
MH  - Cryopreservation
MH  - Female
MH  - Humans
MH  - Persistent Organic Pollutants/*metabolism
MH  - Pilot Projects
MH  - Placenta/*chemistry/metabolism
MH  - Pregnancy
MH  - Specimen Handling/*methods
MH  - Swine
MH  - Thyroid Hormones/*analysis
MH  - Vitamin E/*analysis
MH  - Young Adult
OTO - NOTNLM
OT  - Elements
OT  - Monoamines
OT  - Persistent organic pollutants
OT  - Placental distribution
OT  - Thyroid hormones
OT  - Vitamin E
EDAT- 2020/08/23 06:00
MHDA- 2021/08/11 06:00
CRDT- 2020/08/23 06:00
PHST- 2020/05/19 00:00 [received]
PHST- 2020/07/29 00:00 [revised]
PHST- 2020/08/10 00:00 [accepted]
PHST- 2020/08/23 06:00 [pubmed]
PHST- 2021/08/11 06:00 [medline]
PHST- 2020/08/23 06:00 [entrez]
AID - S0143-4004(20)30262-9 [pii]
AID - 10.1016/j.placenta.2020.08.009 [doi]
PST - ppublish
SO  - Placenta. 2020 Oct;100:45-53. doi: 10.1016/j.placenta.2020.08.009. Epub 2020 Aug 
      11.