PMID- 32828707
OWN - NLM
STAT- MEDLINE
DCOM- 20220114
LR  - 20220114
IS  - 2213-2945 (Print)
IS  - 2213-2953 (Linking)
VI  - 10
IP  - 3
DP  - 2021 May-Jun
TI  - Extraskeletal myxoid chondrosarcoma: combining cytopathology with molecular 
      testing to achieve diagnostic accuracy.
PG  - 293-299
LID - S2213-2945(20)30264-7 [pii]
LID - 10.1016/j.jasc.2020.07.135 [doi]
AB  - INTRODUCTION: Advances in the genetics of soft tissue neoplasia have allowed for 
      the diagnostic recognition of specific tumor types from small biopsy specimens, 
      including those procured using the fine needle aspiration (FNA) biopsy technique. 
      Extraskeletal myxoid chondrosarcoma (EMC) is a malignant mesenchymal neoplasm 
      characterized by NR4A3 and, less specifically, by EWSR1 gene rearrangements. A 
      series of EMC cytologic specimens was examined to demonstrate the diagnostic 
      value of incorporating fluorescence in situ hybridization (FISH) testing in 
      cytologic cases of suspected EMC. MATERIALS AND METHODS: A search was made of our 
      cytopathology and surgical pathology databases for cases diagnosed as EMC. FNA 
      biopsy cytology, exfoliative cytology, imprint cytology, and FISH analysis were 
      performed and examined using standard techniques. RESULTS: A total of 16 cases of 
      EMC were retrieved from 15 patients (male/female ratio, 2.8:1; mean age, 62 
      years). Of the 15 patients, 10 were new patients with primary tumors, 2 had 
      locally recurrent tumors, and 4 had metastases. The sites included the 
      extremities in 10 cases, the trunk in 4, serous effusion in 1, and a mediastinal 
      lymph node in 1 case. The specific cytologic diagnoses were EMC (14 cases; 88%), 
      suspicious for EMC (n = 1), and malignant cells (n = 1). All cases for which FISH 
      testing was successfully used were specifically recognized as EMC. Aspirates and 
      imprint smears consisted of uniformly rounded cells set in an opaque 
      myxoid/chondromyxoid stroma (less abundant and more diaphanous in the effusion 
      sample), sometimes arranged in short anastomosing cords. FNA of 1 case of an EMC 
      cellular variant mimicked a malignant small rounded cell tumor. CONCLUSION: EMC 
      can be added to the growing list of soft tissue neoplasms that are specifically 
      recognizable using cytopathology, coupled with judicious application of ancillary 
      molecular testing.
CI  - Copyright (c) 2020 American Society of Cytopathology. Published by Elsevier Inc. 
      All rights reserved.
FAU - Wakely, Paul E Jr
AU  - Wakely PE Jr
AD  - Department of Pathology, James Cancer Hospital and Solove Research Institute, The 
      Ohio State University Wexner Medical Center, Columbus, Ohio. Electronic address: 
      paul.wakely@osumc.edu.
LA  - eng
PT  - Journal Article
DEP - 20200731
PL  - United States
TA  - J Am Soc Cytopathol
JT  - Journal of the American Society of Cytopathology
JID - 101613234
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (EWSR1 protein, human)
RN  - 0 (NR4A3 protein, human)
RN  - 0 (RNA-Binding Protein EWS)
RN  - 0 (Receptors, Steroid)
RN  - 0 (Receptors, Thyroid Hormone)
RN  - Chondrosarcoma, Extraskeletal Myxoid
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biopsy, Fine-Needle
MH  - Chondrosarcoma/*diagnosis/*genetics/pathology
MH  - DNA-Binding Proteins/genetics
MH  - Female
MH  - Gene Rearrangement
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Male
MH  - Middle Aged
MH  - Molecular Diagnostic Techniques/*methods
MH  - Neoplasms, Connective and Soft Tissue/*diagnosis/*genetics/pathology
MH  - RNA-Binding Protein EWS/genetics
MH  - Receptors, Steroid/genetics
MH  - Receptors, Thyroid Hormone/genetics
MH  - Soft Tissue Neoplasms/*diagnosis/*genetics/pathology
OTO - NOTNLM
OT  - EWSR1
OT  - Extraskeletal myxoid chondrosarcoma
OT  - Fine needle aspiration biopsy
OT  - Fluorescence in situ hybridization
OT  - NR4A3
EDAT- 2020/08/24 06:00
MHDA- 2022/01/15 06:00
CRDT- 2020/08/24 06:00
PHST- 2020/06/19 00:00 [received]
PHST- 2020/07/21 00:00 [revised]
PHST- 2020/07/21 00:00 [accepted]
PHST- 2020/08/24 06:00 [pubmed]
PHST- 2022/01/15 06:00 [medline]
PHST- 2020/08/24 06:00 [entrez]
AID - S2213-2945(20)30264-7 [pii]
AID - 10.1016/j.jasc.2020.07.135 [doi]
PST - ppublish
SO  - J Am Soc Cytopathol. 2021 May-Jun;10(3):293-299. doi: 10.1016/j.jasc.2020.07.135. 
      Epub 2020 Jul 31.