PMID- 32831621
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240329
IS  - 1369-703X (Print)
IS  - 1369-703X (Linking)
VI  - 150
DP  - 2019 Oct 15
TI  - Effect of Monocyte Seeding Density on Dendritic Cell Generation in an Automated 
      Perfusion-Based Culture System.
LID - 107291 [pii]
LID - 10.1016/j.bej.2019.107291 [doi]
AB  - Dendritic cells (DCs) are increasingly important for research and clinical use 
      but obtaining sufficient numbers of dendritic cells is a growing challenge. We 
      systemically investigated the effect of monocyte (MO) seeding density on the 
      generation of monocyte-derived immature DCs (iDCs) in MicroDEN, a perfusion-based 
      culture system, as well as 6-well plates. Cell surface markers and the ability of 
      the iDCs to induce proliferation of allogeneic T cells were examined. The data 
      shows a strong relationship between iDC phenotype, specifically CD80/83/86 
      expression, and T cell proliferation. MicroDEN generated iDCs proved better than 
      well plate generated iDCs at inducing T cell proliferation within the 200k-600k 
      MO/cm(2) seeding density range studied. We attribute this to perfusion in 
      MicroDEN which supplies fresh differentiation medium continuously to the 
      differentiating MOs while concurrently removing depleted medium and toxic 
      byproducts of cellular respiration. MicroDEN generated fewer iDCs on a normalized 
      basis than the well plates at lower MO seeding densities but generated equivalent 
      numbers of iDCs at 600k MO seeding density. These results demonstrate that 
      MicroDEN is capable of generating greater numbers of iDCs with less manual work 
      than standard well plate culture and the MicroDEN generated iDCs have greater 
      ability to induce T cell proliferation.
FAU - Kozbial, Andrew
AU  - Kozbial A
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115.
FAU - Bhandary, Lekhana
AU  - Bhandary L
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115.
FAU - Murthy, Shashi K
AU  - Murthy SK
AD  - Northeastern University, Department of Chemical Engineering, Boston, MA 02115.
LA  - eng
GR  - U24 AI118665/AI/NIAID NIH HHS/United States
PT  - Journal Article
DEP - 20190705
PL  - Netherlands
TA  - Biochem Eng J
JT  - Biochemical engineering journal
JID - 9891831
PMC - PMC7441814
MID - NIHMS1535680
OTO - NOTNLM
OT  - Monocyte seeding density
OT  - T cell expansion
OT  - automated cell culture
OT  - dendritic cell functionality
OT  - dendritic cells
OT  - perfusion
EDAT- 2020/08/25 06:00
MHDA- 2020/08/25 06:01
PMCR- 2020/10/15
CRDT- 2020/08/25 06:00
PHST- 2020/08/25 06:00 [entrez]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2020/08/25 06:01 [medline]
PHST- 2020/10/15 00:00 [pmc-release]
AID - 107291 [pii]
AID - 10.1016/j.bej.2019.107291 [doi]
PST - ppublish
SO  - Biochem Eng J. 2019 Oct 15;150:107291. doi: 10.1016/j.bej.2019.107291. Epub 2019 
      Jul 5.