PMID- 32832062
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2090-8113 (Print)
IS  - 2042-0048 (Electronic)
IS  - 2042-0048 (Linking)
VI  - 2020
DP  - 2020
TI  - The Canine Erythrocyte Sedimentation Rate (ESR): Evaluation of a Point-of-Care 
      Testing Device (MINIPET DIESSE).
PG  - 3146845
LID - 10.1155/2020/3146845 [doi]
LID - 3146845
AB  - The erythrocyte sedimentation rate (ESR) in canine medicine has been replaced by 
      the evaluation of a few sensitive markers of the acute-phase proteins. The aim of 
      the study was to evaluate the ESR using a point-of-care (MINIPET, DIESSE 
      Diagnostica Senese S.p.A.) device (ESR-MP) and to compare the results with the 
      gold standard Westergren method (ESR-W) in dogs. One hundred and nineteen K3-EDTA 
      blood samples for complete blood count were randomly selected and assayed for 
      ESR. The reference interval (RI) was established using the percentile method. The 
      coefficient of variation (CV) in intra-assay and interassay precision of ESR-MP 
      was calculated. The analytical sensitivity (Se), specificity (Sp), positive 
      predictive values (PPVs), and negative predictive values (NPVs) were calculated. 
      Agreement between ESR-MP and ESR-W was assessed with Pearson correlation 
      coefficient (r), Cohen concordance test (K), Passing-Bablok regression, and 
      Bland-Altman plots. Ten canine samples (8.4%) were ruled out because of 
      flag-error by the MINIPET instrument (4.2%) or because they showed the diphasic 
      pattern in ESR-W (4.2%). The canine RI of ESR-MP was 0-10 mm/h. Precision was 
      excellent in intra-assay (CV = 0.02) and interassay (CV = 0.32). The analytical 
      characteristics of ESR-MP in nonanemic samples were as follows: Se = 0.82, 
      Sp = 0.95, PPV = 0.82, and NPV = 0.95. The accuracy of ESR-MP was better than 
      ESR-W in nonanemic samples (r = 0.87; K = 0.77) and lower in anemic subjects (Hct 
      <37%) (r = 0.76; K = 0.69). The Passing-Bablok regression showed the presence of 
      systematic error and the absence of proportional error only in nonanemic blood 
      samples. The Bland-Altman plots gave negative average values due to the 
      difference in RIs and an agreement in both ESRs. The ESR-MP results can be 
      obtained with the same K3-EDTA tubes used for the blood count, in shortcut time, 
      and at reduced costs using the MINIPET device. These investigations highlight 
      that ESR-MP could be useful in canine clinical settings.
CI  - Copyright (c) 2020 Carlo Militello et al.
FAU - Militello, Carlo
AU  - Militello C
AD  - Department of Veterinary Science, University of Pisa, Pisa, Italy.
FAU - Pasquini, Anna
AU  - Pasquini A
AD  - Department of Veterinary Science, University of Pisa, Pisa, Italy.
FAU - Medina Valentin, Anyela Andrea
AU  - Medina Valentin AA
AD  - Department of Veterinary Science, University of Pisa, Pisa, Italy.
FAU - Simcic, Petra
AU  - Simcic P
AD  - Department of Veterinary Science, University of Pisa, Pisa, Italy.
FAU - De Feo, Giulia
AU  - De Feo G
AD  - Department of Veterinary Science, University of Pisa, Pisa, Italy.
FAU - Lubas, George
AU  - Lubas G
AUID- ORCID: 0000-0002-9430-1060
AD  - Department of Veterinary Science, University of Pisa, Pisa, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200806
PL  - United States
TA  - Vet Med Int
JT  - Veterinary medicine international
JID - 101524203
PMC - PMC7428833
COIS- All the authors involved in this paper did not have any conflicts of interest.
EDAT- 2020/08/25 06:00
MHDA- 2020/08/25 06:01
PMCR- 2020/08/06
CRDT- 2020/08/25 06:00
PHST- 2020/03/20 00:00 [received]
PHST- 2020/07/14 00:00 [revised]
PHST- 2020/07/18 00:00 [accepted]
PHST- 2020/08/25 06:00 [entrez]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2020/08/25 06:01 [medline]
PHST- 2020/08/06 00:00 [pmc-release]
AID - 10.1155/2020/3146845 [doi]
PST - epublish
SO  - Vet Med Int. 2020 Aug 6;2020:3146845. doi: 10.1155/2020/3146845. eCollection 
      2020.