PMID- 32832321 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20210802 IS - 2190-572X (Print) IS - 2190-5738 (Electronic) IS - 2190-5738 (Linking) VI - 10 IP - 8 DP - 2020 Aug TI - Berberine ameliorates rats model of combined Alzheimer's disease and type 2 diabetes mellitus via the suppression of endoplasmic reticulum stress. PG - 359 LID - 10.1007/s13205-020-02354-7 [doi] LID - 359 AB - This study is aimed to investigate the protective effect against type 2 diabetes mellitus (T2DM) and Alzheimer's disease (AD) of Berberine (BBR), and the underlying mechanism of action is explored. We established a rat model of combined AD and T2DM and used it to investigate the effect of BBR (150 mg/kg) on the course of these pathologies. The Morris water maze, biochemical analysis, hematoxylin-eosin staining, immunohistochemical study, immunofluorescent staining, TUNEL assay, RT-qPCR and western blot were used to reveal the effect of BBR on blood glucose, lipid changes, hippocampal injuries and cognitive impairment. The results showed that BBR could alleviate memory deficits, restore the disordered arrangement of nerve cells, the damage of neurons, improve TUNEL-positive cells and decrease the elevated levels of fasting blood glucose, triglyceride, total cholesterol and glycosylated serum protein levels in Alzheimer diabetic rats. Moreover, BBR treatment reduces the transcription of mRNAs and expression of proteins related to endoplasmic reticulum (ER) stress. These findings conclude that BBR can protect neurons by inhibiting the pathway of ER stress and thereby play an essential role in the preventive and therapeutic of AD and T2DM. CI - (c) King Abdulaziz City for Science and Technology 2020. FAU - Xuan, Wei-Ting AU - Xuan WT AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 AD - Department of Neurology, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031 China. GRID: grid.412679.f. ISNI: 0000 0004 1771 3402 FAU - Wang, Han AU - Wang H AD - Department of Neurology, First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei, 230031 China. GRID: grid.412679.f. ISNI: 0000 0004 1771 3402 FAU - Zhou, Peng AU - Zhou P AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 AD - Institute of Integrated Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, 230012 China. AD - Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012 China. FAU - Ye, Ting AU - Ye T AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 FAU - Gao, Hua-Wu AU - Gao HW AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 FAU - Ye, Shu AU - Ye S AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 AD - Institute of Integrated Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, 230012 China. AD - Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012 China. FAU - Wang, Jing-Hui AU - Wang JH AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 AD - Institute of Integrated Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, 230012 China. AD - Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012 China. FAU - Chen, Meng-Lian AU - Chen ML AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 FAU - Song, Hang AU - Song H AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 FAU - Wang, Yan AU - Wang Y AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 AD - Institute of Integrated Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, 230012 China. AD - Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012 China. FAU - Cai, Biao AU - Cai B AUID- ORCID: 0000-0002-5177-5290 AD - School of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, No.1 Qianjiang Road, Hefei City, 230012 Anhui Province China. GRID: grid.252251.3. ISNI: 0000 0004 1757 8247 AD - Institute of Integrated Chinese and Western Medicine, Anhui Academy of Chinese Medicine, Hefei, 230012 China. AD - Anhui Province Key Laboratory of Chinese Medicinal Formula, Hefei, 230012 China. LA - eng PT - Journal Article DEP - 20200729 PL - Germany TA - 3 Biotech JT - 3 Biotech JID - 101565857 PMC - PMC7391461 OTO - NOTNLM OT - Alzheimer's disease OT - Berberine OT - Endoplasmic reticulum stress OT - Type 2 diabetes mellitus COIS- Conflict of interestThe authors declare no conflict of interest. EDAT- 2020/08/25 06:00 MHDA- 2020/08/25 06:01 PMCR- 2021/08/01 CRDT- 2020/08/25 06:00 PHST- 2020/03/06 00:00 [received] PHST- 2020/07/22 00:00 [accepted] PHST- 2020/08/25 06:00 [entrez] PHST- 2020/08/25 06:00 [pubmed] PHST- 2020/08/25 06:01 [medline] PHST- 2021/08/01 00:00 [pmc-release] AID - 2354 [pii] AID - 10.1007/s13205-020-02354-7 [doi] PST - ppublish SO - 3 Biotech. 2020 Aug;10(8):359. doi: 10.1007/s13205-020-02354-7. Epub 2020 Jul 29.