PMID- 32832486
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201105
IS  - 2251-9637 (Print)
IS  - 2251-9645 (Electronic)
IS  - 2251-9637 (Linking)
VI  - 9
IP  - 1
DP  - 2020 Winter
TI  - Saponins from Tribulus terrestris L. Extract Down-regulate the Expression of 
      ICAM-1, VCAM-1 and E-selectin in Human Endothelial Cell Lines.
PG  - 73-83
LID - 10.22088/IJMCM.BUMS.9.1.73 [doi]
AB  - Atherosclerosis is an inflammatory disease in which intracellular adhesion 
      molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin 
      (SELE) are consistently expressed in the vascular endothelium. Several evidence 
      support the crucial role of adhesion molecules in the development of 
      atherosclerosis and plaque instability. Due to the anti-inflammatory activity of 
      Tribulus terrestris (TT), the present study investigated the effect of aqueous 
      extract and saponin fraction of TT on the expression of ICAM-1, VCAM-1, and SELE 
      genes in endothelial cells during normal and lipopolysaccharide (LPS) induced 
      conditions. Human umbilical vein endothelial cells (HUVEC) and human bone marrow 
      endothelial cells (HBMEC) were cultured, stimulated by LPS, and treated with 
      aqueous extract and saponin fraction of TT. Finally, the expression of ICAM-1, 
      VCAM-1, and SELE genes were measured using quantitative real-time polymerase 
      chain reaction. LPS-induced HUVECs and HBMECs significantly increased the 
      expression of ICAM-1, VCAM-1, and SELE in comparison with control groups 
      (P<0.001). Treatment of LPS-induced HUVECs and HBMECs by aqueous extract and 
      saponin fraction of TT decreased the expression of all three mentioned genes 
      significantly (P<0.001) in comparison with LPS-induced cells. Taken together, our 
      data suggest that TT has an anti-in fl ammatory effect. In vivo study about 
      anti-in fl ammatory effect of this herb may provide new insights into the 
      development of a herbal drug for the prevention/therapy of atherosclerosis.
FAU - Fereydouni, Zahra
AU  - Fereydouni Z
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Amirinezhad Fard, Elahe
AU  - Amirinezhad Fard E
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Mansouri, Kamran
AU  - Mansouri K
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Mohammadi Motlagh, Hamid-Reza
AU  - Mohammadi Motlagh HR
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Mostafaie, Ali
AU  - Mostafaie A
AD  - Medical Biology Research Center, Health Technology Institute, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Int J Mol Cell Med
JT  - International journal of molecular and cellular medicine
JID - 101598104
PMC - PMC7422852
OTO - NOTNLM
OT  - Atherosclerosis
OT  - Tribulus terrestris L.
OT  - adhesion molecules
OT  - gene expression
OT  - human endothelial cells
OT  - saponin
EDAT- 2020/08/25 06:00
MHDA- 2020/08/25 06:01
PMCR- 2020/01/01
CRDT- 2020/08/25 06:00
PHST- 2020/08/25 06:00 [entrez]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2020/08/25 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.22088/IJMCM.BUMS.9.1.73 [doi]
PST - ppublish
SO  - Int J Mol Cell Med. 2020 Winter;9(1):73-83. doi: 10.22088/IJMCM.BUMS.9.1.73.