PMID- 32833207
OWN - NLM
STAT- MEDLINE
DCOM- 20210322
LR  - 20210714
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2203
DP  - 2020
TI  - The Preparation of Chicken Tracheal Organ Cultures and Their Application for 
      Ciliostasis Test, Growth Kinetics Studies, and Virus Propagation.
PG  - 97-106
LID - 10.1007/978-1-0716-0900-2_8 [doi]
AB  - Chicken tracheal organ cultures (TOCs) provide a simple ex vivo system that makes 
      use of transverse section of tracheal rings extracted from embryos or adult birds 
      to perform classical virological techniques for virus isolation, propagation and 
      titrations, alongside with gene-expression analysis and virus-host interaction 
      studies. Most IBV strains replicate well in TOCs, thus conveniently allowing 
      growth kinetics analysis. Viral replication is revealed by observation of 
      ciliostasis as marker of infection in tracheas extracted from birds ex vivo, as 
      well as in vitro analysis providing a reliable infection model and a useful tool 
      for titration.
FAU - Dowgier, Giulia
AU  - Dowgier G
AD  - The Pirbright Institute, Surrey, UK.
FAU - Bickerton, Erica
AU  - Bickerton E
AD  - The Pirbright Institute, Surrey, UK. erica.bickerton@pirbright.ac.uk.
LA  - eng
GR  - BBS/E/I/00007034/BB_/Biotechnology and Biological Sciences Research 
      Council/United Kingdom
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques/methods
MH  - Cells, Cultured
MH  - Chickens
MH  - Epithelial Cells/metabolism/virology
MH  - Infectious bronchitis virus/isolation & purification
MH  - Kinetics
MH  - Organ Culture Techniques/*methods
MH  - Primary Cell Culture/methods
MH  - Trachea/*metabolism/virology
MH  - Virus Cultivation/*methods
MH  - Virus Replication/physiology
OTO - NOTNLM
OT  - Ciliostasis
OT  - Respiratory virus
OT  - Tracheal organ cultures
OT  - Virus passaging
OT  - Virus titration
EDAT- 2020/08/25 06:00
MHDA- 2021/03/23 06:00
CRDT- 2020/08/25 06:00
PHST- 2020/08/25 06:00 [entrez]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2021/03/23 06:00 [medline]
AID - 10.1007/978-1-0716-0900-2_8 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2203:97-106. doi: 10.1007/978-1-0716-0900-2_8.