PMID- 32833280
OWN - NLM
STAT- MEDLINE
DCOM- 20210427
LR  - 20210427
IS  - 1530-6860 (Electronic)
IS  - 0892-6638 (Linking)
VI  - 34
IP  - 11
DP  - 2020 Nov
TI  - miR-124-3p increases in high glucose induced osteocyte-derived exosomes and 
      regulates galectin-3 expression: A possible mechanism in bone remodeling 
      alteration in diabetic periodontitis.
PG  - 14234-14249
LID - 10.1096/fj.202000970RR [doi]
AB  - The mechanisms underlying the two-way relationship between diabetes mellitus (DM) 
      and periodontitis are unclear. We examined a possible effect of galectin-3 
      (Gal-3), a factor in DM and bone metabolism, on periodontitis with or without DM. 
      Using enzyme-linked immunosorbent assay, we detected saliva Gal-3 in patients 
      with periodontitis, with or without type 2 diabetes mellitus (T2DM). In animal 
      models, we measured periodontal bone microarchitecture via micro computed 
      tomography, and detected Gal-3, Runt-related transcription factor 2 (Runx2), and 
      interleukin-6 (IL-6) expression in alveolar bone. Applying dual luciferase 
      reporter assay, we explored the target binding of miR-124-3p and Gal-3. We 
      examined osteocyte-derived exosomes with transmission electron microscopy and 
      detected miR-124-3p, Gal-3, and IL-6 expression in exosomes. Saliva Gal-3 was 
      increased in DM compared with controls but decreased in patients with moderate 
      periodontitis and DM compared with those who had moderate periodontitis only. 
      Alveolar bone mass was increased in DM and exacerbated in DM with periodontitis. 
      Gal-3 and Runx2 were both increased in periodontitis and DM compared with 
      controls, but decreased in DM with periodontitis compared with DM alone. 
      MiR-124-3p targeted and inhibited Gal-3 expression in vitro. Osteocytes secreted 
      exosomes carrying miR-124-3p, Gal-3, and IL-6, which were influenced by high 
      glucose. These findings indicate that osteocyte-derived exosomes carrying 
      miR-124-3p may regulate Gal-3 expression of osteoblasts, especially under 
      high-glucose conditions, suggesting a possible mechanism for DM-related alveolar 
      bone pathologies.
CI  - (c) 2020 The Authors. The FASEB Journal published by Wiley Periodicals LLC on 
      behalf of Federation of American Societies for Experimental Biology.
FAU - Li, Jun
AU  - Li J
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Guo, Yue
AU  - Guo Y
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Chen, Ying-Yi
AU  - Chen YY
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Liu, Qiong
AU  - Liu Q
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Chen, Yun
AU  - Chen Y
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Tan, Li
AU  - Tan L
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Zhang, Shao-Hui
AU  - Zhang SH
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Gao, Zheng-Rong
AU  - Gao ZR
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Zhou, Ying-Hui
AU  - Zhou YH
AD  - Department of Endocrinology, The Second Xiangya Hospital, Central South 
      University, Changsha, China.
FAU - Zhang, Gui-Ying
AU  - Zhang GY
AD  - Department of Dermatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
FAU - Feng, Yun-Zhi
AU  - Feng YZ
AD  - Department of Stomatology, The Second Xiangya Hospital, Central South University, 
      Changsha, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200824
PL  - United States
TA  - FASEB J
JT  - FASEB journal : official publication of the Federation of American Societies for 
      Experimental Biology
JID - 8804484
RN  - 0 (Galectin 3)
RN  - 0 (MIRN124 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Alveolar Bone Loss/etiology/metabolism/*pathology
MH  - Animals
MH  - Bone Remodeling
MH  - Diabetes Mellitus, Experimental/physiopathology
MH  - Diabetes Mellitus, Type 2/*physiopathology
MH  - Exosomes/drug effects/genetics/*metabolism
MH  - Female
MH  - Galectin 3/genetics/*metabolism
MH  - Gene Expression Regulation
MH  - Glucose/pharmacology
MH  - Humans
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Osteoblasts/drug effects/metabolism/*pathology
MH  - Periodontitis/*complications/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - alveolar bone
OT  - diabetes mellitus
OT  - inflammation
OT  - osteoblast
OT  - saliva
EDAT- 2020/08/25 06:00
MHDA- 2021/04/28 06:00
CRDT- 2020/08/25 06:00
PHST- 2020/04/21 00:00 [received]
PHST- 2020/07/26 00:00 [revised]
PHST- 2020/07/29 00:00 [accepted]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2021/04/28 06:00 [medline]
PHST- 2020/08/25 06:00 [entrez]
AID - 10.1096/fj.202000970RR [doi]
PST - ppublish
SO  - FASEB J. 2020 Nov;34(11):14234-14249. doi: 10.1096/fj.202000970RR. Epub 2020 Aug 
      24.