PMID- 32835933
OWN - NLM
STAT- MEDLINE
DCOM- 20210803
LR  - 20210803
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 67
DP  - 2020 Dec
TI  - Comparative evaluation of two anticoagulants used for the analysis of 
      haematological, biochemical parameters and blood cell morphology of himalayan 
      snow trout, Schizopyge plagiostomus.
PG  - 101398
LID - S0040-8166(20)30145-2 [pii]
LID - 10.1016/j.tice.2020.101398 [doi]
AB  - The aim of the present study is to evaluate the effect of lithium heparin and 
      K(2)EDTA on haematology, biochemical parameters and blood cell morphology of snow 
      trout Schizopyge plagiostomus inhabiting in Kashmir Himalayan region. The 
      haematological parameters such as hemoglobin (Hb), red blood cellcount (RBC), 
      white blood cell (WBC), haematocrit (Hct), erythrocyte indices; mean corpuscular 
      hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), mean 
      corpuscular volume (MCV), produced certain significant differences in their 
      values with respect to both anticoagulants. Higher values of Hct, MCH and MCV 
      while lower RBC count and MCHC values were recorded with a sample treated with 
      K(2)EDTA compared to sample treated with lithium heparin. However, no significant 
      change in Hb and WBC count was observed in the values of samples treated with 
      both the anticoagulants. In the present study, the effect of two anticoagulant in 
      biochemical parameters revealed that the alkaline phosphatase (ALP) and aspartate 
      transaminase (AST) produced significant (P < 0.05) differences. Higher AST and 
      lower ALP activity were recorded in the sample treated with K(2)EDTA compared to 
      overall values in serum. Morphometric analysis showed an increase in the RBC 
      perimeter area in samples treated with K(2)EDTA as compared to samples treated 
      with lithium heparin. On the basis of above findings, it is recommended that in 
      fish blood analysis heparin as anticoagulant could be useful as compared to K(2) 
      EDTA.
CI  - Copyright (c) 2020. Published by Elsevier Ltd.
FAU - Sheikh, Zubair Ahmad
AU  - Sheikh ZA
AD  - Fish Nutrition Research Laboratory, Department of Zoology, University of Kashmir, 
      Hazratbal, Srinagar, 190 006 India.
FAU - Ahmed, Imtiaz
AU  - Ahmed I
AD  - Fish Nutrition Research Laboratory, Department of Zoology, University of Kashmir, 
      Hazratbal, Srinagar, 190 006 India. Electronic address: imtiazamu1@yahoo.com.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20200621
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Anticoagulants)
SB  - IM
MH  - Animals
MH  - Anticoagulants/*pharmacology
MH  - Blood Cells/*cytology/drug effects
MH  - Cell Shape/*drug effects
MH  - Erythrocytes/cytology/drug effects
MH  - Geography
MH  - India
MH  - Trout/*blood
OTO - NOTNLM
OT  - Biochemical parameter
OT  - Blood cell morphology
OT  - Haematological parameters
OT  - K(2)EDTA
OT  - Lithium heparin
OT  - Schizopyge plagiostomus
EDAT- 2020/08/25 06:00
MHDA- 2021/08/04 06:00
CRDT- 2020/08/25 06:00
PHST- 2020/03/16 00:00 [received]
PHST- 2020/06/09 00:00 [revised]
PHST- 2020/06/15 00:00 [accepted]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2021/08/04 06:00 [medline]
PHST- 2020/08/25 06:00 [entrez]
AID - S0040-8166(20)30145-2 [pii]
AID - 10.1016/j.tice.2020.101398 [doi]
PST - ppublish
SO  - Tissue Cell. 2020 Dec;67:101398. doi: 10.1016/j.tice.2020.101398. Epub 2020 Jun 
      21.