PMID- 32835939
OWN - NLM
STAT- MEDLINE
DCOM- 20210803
LR  - 20210803
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 67
DP  - 2020 Dec
TI  - The effective role of sodium copper chlorophyllin on the dysfunction of bone 
      marrow mesenchymal stem cells in multiple myeloma via regulating TGF-beta1.
PG  - 101406
LID - S0040-8166(20)30136-1 [pii]
LID - 10.1016/j.tice.2020.101406 [doi]
AB  - BACKGROUND: The osteoblast differentiation of bone marrow-derived stem cells 
      (BMSCs) is impaired in multiple myeloma (MM). We investigated the effects of 
      sodium copper chlorophyllin (SCC) on osteoblast differentiation ability of BMSCs 
      from MM. METHODS: Clinical bone marrow samples were collected. Fluorescence 
      Activated Cell Sorter (FACS) was used to identify surface markers of BMSCs. BMSCs 
      were treated with different concentrations of SCC and cell viability was detected 
      by MTT assay. Relative mRNA and protein expressions of transforming growth 
      factor-beta1 (TGF-beta1), SMAD2/3, osteogenic differentiation indicators (RUNX2 and 
      OCN) were measured by quantitative real-time polymerase chain reaction (qRT-PCR) 
      and Western blot. Alkaline phosphatase (ALP) was stained for activity detection. 
      Formation of calcium nodus of BMSCs was examined by Alizarin Red S staining. 
      RESULTS: CD90 and CD105 were high-expressed, but CD34 and CD45 were not expressed 
      in BMSCs. BMSCs in MM group showed a lower expression of TGF-beta1 and a lower 
      degree of osteogenic differentiation. SCC enhanced activities of BMSCs, ALP 
      activity, and formation of calcium nodus, activated TGF-beta1, SMAD2/3 pathway and 
      increased RUNX2 and OCN expressions in BMSCs. Silencing TGF-beta1 reversed the 
      effects of SCC on BMSCs in MM. CONCLUSION: SCC could effectively improve the 
      proliferation and osteogenic differentiation of BMSCs in MM through regulating 
      TGF-beta1.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Su, Chuanyong
AU  - Su C
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China.
FAU - Tao, Diehong
AU  - Tao D
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China.
FAU - Ren, Li
AU  - Ren L
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China.
FAU - Guo, Shuping
AU  - Guo S
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China.
FAU - Zhou, Wenfei
AU  - Zhou W
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China.
FAU - Wu, Haiying
AU  - Wu H
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China.
FAU - Jiang, Huifang
AU  - Jiang H
AD  - Department of Hematology, Tongde Hospital of Zhejiang Province, No. 234, Gucui 
      Road, Xihu District, Hangzhou, Zhejiang Province, 310012, China. Electronic 
      address: huifang_jhfa@163.com.
LA  - eng
PT  - Journal Article
DEP - 20200707
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Biomarkers)
RN  - 0 (Chlorophyllides)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Smad2 Protein)
RN  - 0 (Smad3 Protein)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EEM82VOY7C (chlorophyllin)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Biomarkers/metabolism
MH  - Bone Marrow Cells/drug effects/*metabolism
MH  - Cell Differentiation/drug effects
MH  - Cell Survival/drug effects
MH  - Chlorophyllides/*pharmacology
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - Gene Silencing/drug effects
MH  - Humans
MH  - Mesenchymal Stem Cells/drug effects/*metabolism
MH  - Multiple Myeloma/*metabolism/pathology
MH  - Osteocalcin/metabolism
MH  - Osteogenesis/drug effects
MH  - RNA, Small Interfering/metabolism
MH  - Signal Transduction/drug effects
MH  - Smad2 Protein/metabolism
MH  - Smad3 Protein/metabolism
MH  - Transforming Growth Factor beta1/*metabolism
OTO - NOTNLM
OT  - Bone marrow mesenchymal stem cells
OT  - Osteogenic differentiation
OT  - Sodium copper chlorophyllin
OT  - TGF-beta1
EDAT- 2020/08/25 06:00
MHDA- 2021/08/04 06:00
CRDT- 2020/08/25 06:00
PHST- 2020/03/11 00:00 [received]
PHST- 2020/07/02 00:00 [revised]
PHST- 2020/07/06 00:00 [accepted]
PHST- 2020/08/25 06:00 [pubmed]
PHST- 2021/08/04 06:00 [medline]
PHST- 2020/08/25 06:00 [entrez]
AID - S0040-8166(20)30136-1 [pii]
AID - 10.1016/j.tice.2020.101406 [doi]
PST - ppublish
SO  - Tissue Cell. 2020 Dec;67:101406. doi: 10.1016/j.tice.2020.101406. Epub 2020 Jul 
      7.