PMID- 3284371
OWN - NLM
STAT- MEDLINE
DCOM- 19880602
LR  - 20190912
IS  - 0093-7002 (Print)
IS  - 0093-7002 (Linking)
VI  - 65
IP  - 2
DP  - 1988 Feb
TI  - Relative allergenic potential of four proteases used as contact lens cleaners.
PG  - 70-5
AB  - The general use of enzymatic cleaners for soft contact lenses has led to reports 
      of incidents of allergic reactions in previously sensitized patients. In light of 
      this, experiments were performed to assess the relative allergenic potential of 
      four such enzymes: papain, subtilisin A, subtilisin B, and pancreatin. Mice 
      immunized intraperitoneally (i.p.) with either subtilisin A or papain exhibited 
      little serum immunoglobulin E (IgE), even after tertiary doses of protein. 
      Primates immunized intradermally (i.d.) with the four enzymes showed various 
      levels of response to challenges: none to papain, mild to moderate to subtilisin 
      A and pancreatin, and severe to subtilisin B. Finally, guinea pigs instilled 
      ocularly with enzymes weekly for 15 weeks exhibited significant hyperemia and 
      chemosis only to the positive control protein, ovalbumin, and not to any of the 
      enzymes tested. We conclude that three of the four enzymes tested have low 
      potential to sensitize human beings, and, further, that animal models may find 
      increased use in screening products of biotechnology.
FAU - Barton, B E
AU  - Barton BE
AD  - Allergan, Inc., Irvine, California.
FAU - James, L C
AU  - James LC
FAU - Spencer, J
AU  - Spencer J
FAU - Malley, A
AU  - Malley A
FAU - Huth, S
AU  - Huth S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Am J Optom Physiol Opt
JT  - American journal of optometry and physiological optics
JID - 0417614
RN  - 0 (Allergens)
RN  - 0 (Detergents)
RN  - 0 (Surface-Active Agents)
RN  - EC 3.4.- (Peptide Hydrolases)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - *Contact Lenses
MH  - *Detergents
MH  - Eye/immunology
MH  - Guinea Pigs
MH  - Immunization
MH  - Macaca mulatta
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Peptide Hydrolases/*immunology
MH  - *Surface-Active Agents
EDAT- 1988/02/01 00:00
MHDA- 1988/02/01 00:01
CRDT- 1988/02/01 00:00
PHST- 1988/02/01 00:00 [pubmed]
PHST- 1988/02/01 00:01 [medline]
PHST- 1988/02/01 00:00 [entrez]
AID - 10.1097/00006324-198802000-00002 [doi]
PST - ppublish
SO  - Am J Optom Physiol Opt. 1988 Feb;65(2):70-5. doi: 
      10.1097/00006324-198802000-00002.