PMID- 32845053
OWN - NLM
STAT- MEDLINE
DCOM- 20210517
LR  - 20210517
IS  - 1612-1880 (Electronic)
IS  - 1612-1872 (Linking)
VI  - 17
IP  - 10
DP  - 2020 Oct
TI  - The Anti-Inflammatory Properties of Rhododendron molle Leaf Extract in 
      LPS-Induced RAW264.7.
PG  - e2000477
LID - 10.1002/cbdv.202000477 [doi]
AB  - Rhododendron molle G.Don is a well-known traditional medicine which has been used 
      to treat rheumatic inflammation. In this study, an inflammatory model of 
      lipopolysaccharide (LPS)-stimulated RAW264.7 cells was established to analyze the 
      anti-inflammatory effect and potential mechanism of the methanol extract of R. 
      molle leaves (RLE). The production of NO and the expression of tumor necrosis 
      factor by LPS were detected by Griess reaction and enzyme linked immunosorbent 
      assay (ELISA). The mRNA expression of TNF-alpha, IL-1beta, IL-6, COX-2 and iNOS was 
      measured by qRT-PCR assay. Griess and qRT-PCR showed that the RLE could 
      significantly concentration-dependently inhibit NO production and the expression 
      of many pro-inflammatory cytokines and inflammatory-related enzymes. Scanning 
      electron microscope (SEM) analysis indicated that RLE could inhibit 
      LPS-stimulated RAW264.7 macrophages activation. The protein level of TNF-alpha and 
      IL-1beta were decreased over 50 % at 100 mug/ml of RLE, as detected by ELISA. These 
      results indicated that RLE had strong anti-inflammatory and immunomodulatory 
      activity.
CI  - (c) 2020 Wiley-VHCA AG, Zurich, Switzerland.
FAU - Zong, Luye
AU  - Zong L
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
FAU - Zhang, Jin
AU  - Zhang J
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
FAU - Dai, Liangfang
AU  - Dai L
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
FAU - Liu, Jian
AU  - Liu J
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
FAU - Yang, Yan
AU  - Yang Y
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
FAU - Xie, Jiankun
AU  - Xie J
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
FAU - Luo, Xiangdong
AU  - Luo X
AD  - College of Life Science, Jiangxi Normal University, Nanchang, 330022, P. R. 
      China.
LA  - eng
GR  - 31360147/National Natural Science Foundation of China/
GR  - 31660384/National Natural Science Foundation of China/
GR  - 20171BAB204021/Natural Science Foundation of Jiangxi Province, China/
GR  - 20202ACB205001/Natural Science Foundation of Jiangxi Province, China/
GR  - YC2017-S150/Innovation Project of Graduate Students in Jiangxi Province, China/
PT  - Journal Article
DEP - 20200921
PL  - Switzerland
TA  - Chem Biodivers
JT  - Chemistry & biodiversity
JID - 101197449
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Plant Extracts)
RN  - 31C4KY9ESH (Nitric Oxide)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/chemistry/isolation & purification/*pharmacology
MH  - Cell Survival/drug effects
MH  - Cytokines/antagonists & inhibitors/biosynthesis
MH  - Lipopolysaccharides/*antagonists & inhibitors/pharmacology
MH  - Macrophages/drug effects/metabolism
MH  - Mice
MH  - Nitric Oxide/antagonists & inhibitors/biosynthesis
MH  - Plant Extracts/chemistry/isolation & purification/*pharmacology
MH  - Plant Leaves/*chemistry
MH  - RAW 264.7 Cells
MH  - Rhododendron/*chemistry
OTO - NOTNLM
OT  - RAW264.7
OT  - Rhododendron molle
OT  - anti-inflammatory activity
OT  - immunomodulation
OT  - pro-inflammatory cytokines
EDAT- 2020/08/28 06:00
MHDA- 2021/05/18 06:00
CRDT- 2020/08/27 06:00
PHST- 2020/06/15 00:00 [received]
PHST- 2020/08/24 00:00 [accepted]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2021/05/18 06:00 [medline]
PHST- 2020/08/27 06:00 [entrez]
AID - 10.1002/cbdv.202000477 [doi]
PST - ppublish
SO  - Chem Biodivers. 2020 Oct;17(10):e2000477. doi: 10.1002/cbdv.202000477. Epub 2020 
      Sep 21.