PMID- 32845828
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20240330
IS  - 1466-5034 (Electronic)
IS  - 1466-5026 (Print)
IS  - 1466-5026 (Linking)
VI  - 70
IP  - 10
DP  - 2020 Oct
TI  - Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., 
      isolated from the gut of the fungus growing-termite Macrotermes natalensis.
PG  - 5255-5262
LID - 10.1099/ijsem.0.004403 [doi]
AB  - The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, 
      designated strains RB29(T) and RB68(T), were determined using a polyphasic 
      approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic 
      neighbours of RB29(T) were identified as Actinomadura rayongensis DSM 102126(T) 
      (99.2 % similarity) and Actinomadura atramentaria DSM 43919(T) (98.7 %), and for 
      strain RB68(T) was Actinomadura hibisca DSM 44148(T) (98.3 %). Digital DNA-DNA 
      hybridization (dDDH) between RB29(T) and its closest phylogenetic neighbours, A. 
      rayongensis DSM 102126(T) and A. atramentaria DSM 43919(T), resulted in 
      similarity values of 53.2 % (50.6-55.9 %) and 26.4 % (24.1-28.9 %), respectively. 
      Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for A. 
      rayongensis DSM 102126(T) and 82.3 % (78.9 %) for A. atramentaria DSM 43919(T). 
      dDDH analysis between strain RB68(T) and A. hibisca DSM 44148(T) gave a 
      similarity value of 24.5 % (22.2-27.0 %). Both strains, RB29(T) and RB68(T), 
      revealed morphological characteristics and chemotaxonomic features typical for 
      the genus Actinomadura, such as the presence of meso-diaminopimelic acid in the 
      cell wall, galactose and glucose as major sugar components within whole-cell 
      hydrolysates and the absence of mycolic acids. The major phospholipids were 
      diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and 
      phosphatidylinositol mannoside. Predominant menaquinones were MK-9(H(6)) and 
      MK-9(H(8)) for RB29(T) and MK-9(H(4)) and MK-9(H(6)) for RB68(T). The main fatty 
      acids were identified as 10-methyloctadecanoic acid (10-methyl C(18:0)), 
      14-methylpentadecanoic acid (iso-C(16:0)), hexadecanoic acid (C(16:0)) and 
      cis-9-octadecanoic acid (C(18 : 1) omega9c). Here, we propose two novel species of 
      the genus Actinomadura: Actinomadura rubteroloni sp. nov. with the type strain 
      RB29(T) (=CCUG 72668(T)=NRRL B-65537(T)) and Actinomadura macrotermitis sp. nov. 
      with the type strain RB68(T) (=CCUG 72669(T)=NRRL B-65538(T)).
FAU - Benndorf, Rene
AU  - Benndorf R
AD  - Chemical Biology of Microbe-Host Interactions, Leibniz Institute for Natural 
      Product Research and Infection Biology e. V., Hans-Knoll-Institute, 
      Beutenbergstrasse 11a, 07745 Jena, Germany.
FAU - Martin, Karin
AU  - Martin K
AD  - Bio Pilot Plant, Leibniz Institute for Natural Product Research and Infection 
      Biology e. V., Hans-Knoll-Institute, Beutenbergstrasse 11a, 07745 Jena, Germany.
FAU - Kufner, Michelle
AU  - Kufner M
AD  - Chemical Biology of Microbe-Host Interactions, Leibniz Institute for Natural 
      Product Research and Infection Biology e. V., Hans-Knoll-Institute, 
      Beutenbergstrasse 11a, 07745 Jena, Germany.
FAU - de Beer, Z Wilhelm
AU  - de Beer ZW
AD  - Department of Biochemistry, Genetics and Microbiology, Forestry and Agriculture 
      Biotechnology Institute, University of Pretoria, Pretoria, South Africa.
FAU - Vollmers, John
AU  - Vollmers J
AD  - Institute for Biological Interfaces 5, Karlsruhe Institute of Technology, 
      Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
FAU - Kaster, Anne-Kristin
AU  - Kaster AK
AD  - Institute for Biological Interfaces 5, Karlsruhe Institute of Technology, 
      Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
FAU - Beemelmanns, Christine
AU  - Beemelmanns C
AD  - Chemical Biology of Microbe-Host Interactions, Leibniz Institute for Natural 
      Product Research and Infection Biology e. V., Hans-Knoll-Institute, 
      Beutenbergstrasse 11a, 07745 Jena, Germany.
LA  - eng
PT  - Journal Article
DEP - 20200826
PL  - England
TA  - Int J Syst Evol Microbiol
JT  - International journal of systematic and evolutionary microbiology
JID - 100899600
RN  - 0 (DNA, Bacterial)
RN  - 0 (Fatty Acids)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 11032-49-8 (Vitamin K 2)
RN  - 523-39-7 (menaquinone 9)
RN  - 583-93-7 (Diaminopimelic Acid)
SB  - IM
MH  - Actinobacteria/*classification/isolation & purification
MH  - Animals
MH  - Bacterial Typing Techniques
MH  - Base Composition
MH  - DNA, Bacterial/genetics
MH  - Diaminopimelic Acid/chemistry
MH  - Fatty Acids/chemistry
MH  - Gastrointestinal Microbiome
MH  - Isoptera/*microbiology
MH  - Nucleic Acid Hybridization
MH  - *Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - South Africa
MH  - Vitamin K 2/analogs & derivatives/chemistry
PMC - PMC7660899
OTO - NOTNLM
OT  - Actinomadura
OT  - Macrotermes natalensis
OT  - new natural products
OT  - termite gut
COIS- The authors declare that there are no conflicts of interest.
EDAT- 2020/08/28 06:00
MHDA- 2020/11/11 06:00
PMCR- 2020/08/26
CRDT- 2020/08/27 06:00
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2020/08/27 06:00 [entrez]
PHST- 2020/08/26 00:00 [pmc-release]
AID - 004403 [pii]
AID - 10.1099/ijsem.0.004403 [doi]
PST - ppublish
SO  - Int J Syst Evol Microbiol. 2020 Oct;70(10):5255-5262. doi: 
      10.1099/ijsem.0.004403. Epub 2020 Aug 26.