PMID- 32847353
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 2379-3694 (Electronic)
IS  - 2379-3694 (Linking)
VI  - 5
IP  - 9
DP  - 2020 Sep 25
TI  - Sensing Adenosine and ATP by Aptamers and Gold Nanoparticles: Opposite Trends of 
      Color Change from Domination of Target Adsorption Instead of Aptamer Binding.
PG  - 2885-2893
LID - 10.1021/acssensors.0c01169 [doi]
AB  - The 27 mer DNA aptamer for adenosine and adenosine 5'-triphosphate (ATP) is a 
      popular model system for designing biosensors. Various strategies have been 
      reported for label-free colorimetric detection using gold nanoparticles (AuNPs). 
      It is generally accepted that free aptamers can protect AuNPs against 
      salt-induced aggregation, whereas target-bound aptamers cannot. However, these 
      studies only considered the aptamer binding to its target, and the adsorption of 
      the aptamer on AuNPs, but none considered the adsorption of target molecules by 
      AuNPs. We herein report that the adsorption of adenosine destabilized 
      citrate-capped AuNPs with an apparent K(d) of just 7.7 muM adenosine, whereas that 
      of ATP stabilized the AuNPs because of the negative charges from the triphosphate 
      group. The adsorbed ATP inhibited the adsorption of DNA. Using the aptamer and a 
      nonbinding mutant, ATP and guanosine 5'-triphosphate (GTP) had the same 
      colorimetric response, and so did adenosine and guanosine, regardless of the DNA 
      sequence, indicating that the color change mainly reflected the adsorption of the 
      nucleosides and nucleotides instead of aptamer binding. The related literature 
      examples using this aptamer were classified into three types and individually 
      analyzed, where the reported color changes can all be explained by the adsorption 
      of target analytes.
FAU - Zhang, Fang
AU  - Zhang F
AD  - College of Biological Science and Engineering, Fuzhou University, Fuzhou 350108, 
      People's Republic of China.
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo, Waterloo N2L 3G1, Ontario, Canada.
FAU - Huang, Po-Jung Jimmy
AU  - Huang PJ
AUID- ORCID: 0000-0003-3436-9968
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo, Waterloo N2L 3G1, Ontario, Canada.
FAU - Liu, Juewen
AU  - Liu J
AUID- ORCID: 0000-0001-5918-9336
AD  - Department of Chemistry, Waterloo Institute for Nanotechnology, University of 
      Waterloo, Waterloo N2L 3G1, Ontario, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200910
PL  - United States
TA  - ACS Sens
JT  - ACS sensors
JID - 101669031
RN  - 7440-57-5 (Gold)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - K72T3FS567 (Adenosine)
SB  - IM
MH  - Adenosine
MH  - Adenosine Triphosphate
MH  - Adsorption
MH  - *Gold
MH  - *Metal Nanoparticles
OTO - NOTNLM
OT  - DNA
OT  - aptamers
OT  - biosensors
OT  - label-free detection
OT  - oligonucleotides
EDAT- 2020/08/28 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/08/28 06:00
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/08/28 06:00 [entrez]
AID - 10.1021/acssensors.0c01169 [doi]
PST - ppublish
SO  - ACS Sens. 2020 Sep 25;5(9):2885-2893. doi: 10.1021/acssensors.0c01169. Epub 2020 
      Sep 10.