PMID- 3284819
OWN - NLM
STAT- MEDLINE
DCOM- 19880623
LR  - 20181130
IS  - 0019-2805 (Print)
IS  - 1365-2567 (Electronic)
IS  - 0019-2805 (Linking)
VI  - 63
IP  - 4
DP  - 1988 Apr
TI  - Immunolocalization of thymosin alpha 1, thymopoietin and thymulin in mouse thymic 
      epithelial cells at different stages of culture: a light and electron microscopic 
      study.
PG  - 721-7
AB  - The secretory evolution of the thymic hormones (thymulin, thymosin alpha 1 and 
      thymopoietin) in cultured thymic reticuloepithelial cells (TREC) was studied by 
      immunocytochemical techniques using monoclonal anti-thymulin or anti-thymosin 
      alpha 1 and polyclonal anti-thymopoietin antibodies (Ab). The culture of TREC was 
      performed with a medium where L-valine was replaced by D-valine, thus ensuring 
      rapid and selective development of these cells. The number of thymulin, thymosin 
      alpha 1 or thymopoietin-containing cells increased progressively from Day 6 to 
      Day 12 of the culture. The localization of the three thymic hormones within the 
      TREC also varied according to the age of the culture. By light microscopy the 
      staining of the three hormones was localized in some cytoplasmic granules at the 
      beginning of the culture and at Day 90, while at Day 12 it was throughout the 
      cytoplasm. In electron microscopy these localizations corresponded respectively 
      to vacuoles of different sizes and to cytosol. All these results show that the 
      synthesis and excretion of thymulin, thymosin alpha 1 and thymopoietin evolve 
      during the development of TREC in culture.
FAU - Fabien, N
AU  - Fabien N
AD  - Laboratoire d'Immunologie, UFR de Medicine A. Carrel, Lyon, France.
FAU - Auger, C
AU  - Auger C
FAU - Monier, J C
AU  - Monier JC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Immunology
JT  - Immunology
JID - 0374672
RN  - 0 (Thymopoietins)
RN  - 0 (Thymus Hormones)
RN  - 61512-21-8 (Thymosin)
RN  - 9H198D04WL (Thymic Factor, Circulating)
RN  - W0B22ISQ1C (Thymalfasin)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Epithelium/metabolism
MH  - Fluorescent Antibody Technique
MH  - Immunoenzyme Techniques
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microscopy, Electron
MH  - Thymalfasin
MH  - Thymic Factor, Circulating/*analysis
MH  - Thymopoietins/*analysis
MH  - Thymosin/*analogs & derivatives/analysis
MH  - Thymus Gland/*analysis
MH  - Thymus Hormones/*analysis
MH  - Time Factors
PMC - PMC1454785
EDAT- 1988/04/01 00:00
MHDA- 1988/04/01 00:01
PMCR- 1989/04/01
CRDT- 1988/04/01 00:00
PHST- 1988/04/01 00:00 [pubmed]
PHST- 1988/04/01 00:01 [medline]
PHST- 1988/04/01 00:00 [entrez]
PHST- 1989/04/01 00:00 [pmc-release]
PST - ppublish
SO  - Immunology. 1988 Apr;63(4):721-7.