PMID- 32848508
OWN - NLM
STAT- MEDLINE
DCOM- 20210809
LR  - 20210809
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2020
DP  - 2020
TI  - Anti-Inflammatory and Antioxidant Effects of Carpesium cernuum L. Methanolic 
      Extract in LPS-Stimulated RAW 264.7 Macrophages.
PG  - 3164239
LID - 10.1155/2020/3164239 [doi]
LID - 3164239
AB  - A hypernomic reaction or an abnormal inflammatory process could cause a series of 
      diseases, such as cardiovascular disease, neurodegeneration, and cancer. 
      Additionally, oxidative stress has been identified to induce severe tissue injury 
      and inflammation. Carpesium cernuum L. (C. cernuum) is a Chinese folk medicine 
      used for its anti-inflammatory, analgesic, and detoxifying properties. However, 
      the underlying molecular mechanism of C. cernuum in inflammatory and oxidative 
      stress conditions remains largely unknown. The aim of this study was to examine 
      the effects of a methanolic extract of C. cernuum (CLME) on lipopolysaccharide- 
      (LPS-) induced RAW 264.7 mouse macrophages and a sepsis mouse model. The data 
      presented in this study indicated that CLME inhibited LPS-induced production of 
      proinflammatory mediators such as nitric oxide (NO) and prostaglandin E(2) 
      (PGE(2)) in RAW 264.7 cells. CLME treatment also reduced reactive oxygen species 
      (ROS) generation and enhanced the expression of heme oxygenase-1 (HO-1) protein 
      in a dose-dependent manner in the LPS-stimulated RAW 264.7 cells. Moreover, CLME 
      treatment abolished the nuclear translocation of nuclear factor-kappaB (NF-kappaB), 
      enhanced the activation of nuclear factor-erythroid 2 p45-related factor 2 
      (Nrf2), and reduced the expression of extracellular signal-related kinase (ERK) 
      and ERK kinase (MEK) phosphorylation in LPS-stimulated RAW 264.7 cells. These 
      outcomes implied that CLME could be a potential antioxidant and anti-inflammatory 
      agent.
CI  - Copyright (c) 2020 Yea-Jin Park et al.
FAU - Park, Yea-Jin
AU  - Park YJ
AD  - Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju, 
      Gangwon-do 26339, Republic of Korea.
FAU - Cheon, Se-Yun
AU  - Cheon SY
AUID- ORCID: 0000-0002-8173-5898
AD  - Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju, 
      Gangwon-do 26339, Republic of Korea.
AD  - Department of Korean Medical Science, School of Korean Medicine and Healthy Aging 
      Korean Medical Research Center, Pusan National University, Yangsan, Gyeongnam, 
      50612, Republic of Korea.
FAU - Lee, Dong-Sung
AU  - Lee DS
AD  - College of Pharmacy, Chosun University, 309 Pilmun-daero, Dong-gu Gwangju 61452, 
      Republic of Korea.
FAU - Cominguez, Divina C
AU  - Cominguez DC
AD  - Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju, 
      Gangwon-do 26339, Republic of Korea.
FAU - Zhang, Zhiyun
AU  - Zhang Z
AD  - State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, 
      The Chinese Academy of Sciences, Beijing 100093, China.
FAU - Lee, Sangwoo
AU  - Lee S
AD  - International Biological Material Research Center, Korea Research Institute of 
      Bioscience and Biotechnology, Daejeon 34141, Republic of Korea.
FAU - An, Hyo-Jin
AU  - An HJ
AUID- ORCID: 0000-0002-2937-874X
AD  - Department of Pharmacology, College of Korean Medicine, Sangji University, Wonju, 
      Gangwon-do 26339, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20200807
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Antioxidants)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Plant Extracts)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EUY85H477I (thiazolyl blue)
RN  - K7Q1JQR04M (Dinoprostone)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/*pharmacology
MH  - Antioxidants/*pharmacology
MH  - Asteraceae/*metabolism
MH  - Dinoprostone/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Heme Oxygenase-1/metabolism
MH  - Inflammation
MH  - Lipopolysaccharides/*metabolism
MH  - Macrophages/metabolism
MH  - Male
MH  - Membrane Proteins/metabolism
MH  - Methanol
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NF-E2-Related Factor 2/metabolism
MH  - Oxidative Stress
MH  - Plant Extracts/*pharmacology
MH  - RAW 264.7 Cells
MH  - Reactive Oxygen Species
MH  - Sepsis/*metabolism
MH  - Tetrazolium Salts/chemistry
MH  - Thiazoles/chemistry
PMC - PMC7439783
COIS- The authors declare that there is no conflict of interest regarding the 
      publication of this paper.
EDAT- 2020/08/28 06:00
MHDA- 2021/08/10 06:00
PMCR- 2020/08/07
CRDT- 2020/08/28 06:00
PHST- 2020/02/07 00:00 [received]
PHST- 2020/06/20 00:00 [revised]
PHST- 2020/06/30 00:00 [accepted]
PHST- 2020/08/28 06:00 [entrez]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2021/08/10 06:00 [medline]
PHST- 2020/08/07 00:00 [pmc-release]
AID - 10.1155/2020/3164239 [doi]
PST - epublish
SO  - Mediators Inflamm. 2020 Aug 7;2020:3164239. doi: 10.1155/2020/3164239. 
      eCollection 2020.