PMID- 32848807
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-042X (Print)
IS  - 1664-042X (Electronic)
IS  - 1664-042X (Linking)
VI  - 11
DP  - 2020
TI  - Deficiency of Calcitonin Gene-Related Peptide Affects Macrophage Polarization in 
      Osseointegration.
PG  - 733
LID - 10.3389/fphys.2020.00733 [doi]
LID - 733
AB  - Macrophages have been described as a critical cell population regulating bone 
      regeneration and osseointegration, and their polarization phenotype is of 
      particular importance. Several studies have shown that calcitonin gene-related 
      peptide-alpha (CGRP) might modulate macrophage polarization in inflammatory response 
      and bone metabolism. This study aimed to investigate the effect of CGRP on 
      macrophage polarization in titanium osseointegration. In vitro , bone 
      marrow-derived macrophages (BMDMs) from C57BL/6 or CGRP (-) (/) (-) mice were 
      obtained and activated for M1 and M2 polarization. Flow cytometry and real-time 
      PCR were used to evaluate the M1/M2 polarization and inflammatory function. In 
      vivo , mice were divided into 3 groups: wild-type, CGRP (-) (/) (-) , and CGRP 
      (-) (/) (-) mice with CGRP lentivirus. After extraction of the maxillary first 
      molar, 0.6 mm x 1.25 mm titanium implants were emplaced. Bone formation and 
      inflammation levels around implants were then observed and analyzed. The results 
      of flow cytometry demonstrated that CGRP deficiency promoted M1 polarization and 
      inhibited M2 polarization in BMDMs, which was consistent with pro-inflammatory 
      and anti-inflammatory cytokine expression levels in real-time PCR. In vivo , 
      compared with the CGRP (-) (/) (-) group, the CGRP gene transfection group 
      displayed better osseointegration and lower inflammation levels, close to those 
      of the wild-type group. These results revealed that CGRP might play roles in 
      macrophage polarization. In addition, CGRP deficiency could inhibit 
      osseointegration in murine maxillae, while CGRP recovery by lentivirus 
      transfection could improve osseointegration and regulate macrophage phenotype 
      expression.
CI  - Copyright (c) 2020 Yuan, Jiang, Wang, Guo, Gong and Xiang.
FAU - Yuan, Ying
AU  - Yuan Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Oral Implantology, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Jiang, Yixuan
AU  - Jiang Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Oral Implantology, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Wang, Bin
AU  - Wang B
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Oral Implantology, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Guo, Yanjun
AU  - Guo Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Oral Implantology, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Gong, Ping
AU  - Gong P
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Oral Implantology, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
FAU - Xiang, Lin
AU  - Xiang L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, Department of Oral Implantology, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
DEP - 20200731
PL  - Switzerland
TA  - Front Physiol
JT  - Frontiers in physiology
JID - 101549006
PMC - PMC7412000
OTO - NOTNLM
OT  - bone remodeling/regeneration
OT  - cell biology
OT  - cytokine(s)
OT  - dental implant(s)
OT  - histochemistry
OT  - immunity
EDAT- 2020/08/28 06:00
MHDA- 2020/08/28 06:01
PMCR- 2020/07/31
CRDT- 2020/08/28 06:00
PHST- 2020/03/06 00:00 [received]
PHST- 2020/06/08 00:00 [accepted]
PHST- 2020/08/28 06:00 [entrez]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2020/08/28 06:01 [medline]
PHST- 2020/07/31 00:00 [pmc-release]
AID - 10.3389/fphys.2020.00733 [doi]
PST - epublish
SO  - Front Physiol. 2020 Jul 31;11:733. doi: 10.3389/fphys.2020.00733. eCollection 
      2020.