PMID- 32849442
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 11
DP  - 2020
TI  - Microbiomes and Specific Symbionts of Social Spiders: Compositional Patterns in 
      Host Species, Populations, and Nests.
PG  - 1845
LID - 10.3389/fmicb.2020.01845 [doi]
LID - 1845
AB  - Social spiders have remarkably low species-wide genetic diversities, potentially 
      increasing the relative importance of microbial symbionts for host fitness. Here 
      we explore the bacterial microbiomes of three species of social Stegodyphus (S. 
      dumicola, S. mimosarum, and S. sarasinorum), within and between populations, 
      using 16S rRNA gene amplicon sequencing. The microbiomes of the three spider 
      species were distinct but shared similarities in membership and structure. This 
      included low overall diversity (Shannon index 0.5-1.7), strong dominance of 
      single symbionts in individual spiders (McNaughton's dominance index 0.68-0.93), 
      and a core microbiome (>50% prevalence) consisting of 5-7 specific symbionts. The 
      most abundant and prevalent symbionts were classified as Chlamydiales, Borrelia, 
      and Mycoplasma, all representing novel, presumably Stegodyphus-specific lineages. 
      Borrelia- and Mycoplasma-like symbionts were localized by fluorescence in situ 
      hybridization (FISH) in the spider midgut. The microbiomes of individual spiders 
      were highly similar within nests but often very different between nests from the 
      same population, with only the microbiome of S. sarasinorum consistently 
      reflecting host population structure. The weak population pattern in microbiome 
      composition renders microbiome-facilitated local adaptation unlikely. However, 
      the retention of specific symbionts across populations and species may indicate a 
      recurrent acquisition from environmental vectors or an essential symbiotic 
      contribution to spider phenotype.
CI  - Copyright (c) 2020 Busck, Settepani, Bechsgaard, Lund, Bilde and Schramm.
FAU - Busck, Mette Marie
AU  - Busck MM
AD  - Section for Microbiology, Department of Biology, Aarhus University, Aarhus, 
      Denmark.
FAU - Settepani, Virginia
AU  - Settepani V
AD  - Section for Genetics, Ecology and Evolution, Department of Biology, Aarhus 
      University, Aarhus, Denmark.
FAU - Bechsgaard, Jesper
AU  - Bechsgaard J
AD  - Section for Genetics, Ecology and Evolution, Department of Biology, Aarhus 
      University, Aarhus, Denmark.
FAU - Lund, Marie Braad
AU  - Lund MB
AD  - Section for Microbiology, Department of Biology, Aarhus University, Aarhus, 
      Denmark.
FAU - Bilde, Trine
AU  - Bilde T
AD  - Section for Genetics, Ecology and Evolution, Department of Biology, Aarhus 
      University, Aarhus, Denmark.
FAU - Schramm, Andreas
AU  - Schramm A
AD  - Section for Microbiology, Department of Biology, Aarhus University, Aarhus, 
      Denmark.
LA  - eng
PT  - Journal Article
DEP - 20200731
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC7412444
OTO - NOTNLM
OT  - 16S sequencing
OT  - Borrelia
OT  - Mycoplasma
OT  - Stegodyphus
OT  - fluorescence in situ hybridization
OT  - microbiome
OT  - spider
OT  - symbiosis
EDAT- 2020/08/28 06:00
MHDA- 2020/08/28 06:01
PMCR- 2020/07/31
CRDT- 2020/08/28 06:00
PHST- 2020/03/06 00:00 [received]
PHST- 2020/07/14 00:00 [accepted]
PHST- 2020/08/28 06:00 [entrez]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2020/08/28 06:01 [medline]
PHST- 2020/07/31 00:00 [pmc-release]
AID - 10.3389/fmicb.2020.01845 [doi]
PST - epublish
SO  - Front Microbiol. 2020 Jul 31;11:1845. doi: 10.3389/fmicb.2020.01845. eCollection 
      2020.