PMID- 32849887
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 1728-1997 (Print)
IS  - 2252-0589 (Electronic)
IS  - 2252-0589 (Linking)
VI  - 21
IP  - 2
DP  - 2020 Spring
TI  - Effect of prostaglandins E(2) and F(2alpha) on granulosa cell apoptosis in goat 
      ovarian follicles.
PG  - 97-102
AB  - BACKGROUND: The goat is a multi-purpose animal producing meat, milk, hide, fiber 
      and manure, and hence plays a significant role in providing supplementary income 
      and livelihood to the huge number of asset-poor ranchers and landless workers of 
      rural India. Aims: The present study is aimed to investigate the in vitro effects 
      of prostaglandins E(2) (PGE(2)) and F(2alpha) (PGF(2alpha)) on ovarian granulosa cells of 
      a goat. METHODS: The healthy, slightly atretic and atretic antral follicles of 
      goats with diameter ranging from 3-8 mm were cultured with PGE(2) and PGF(2alpha) 
      (1.0 mug/ml) along with control for 24 h. Histomorphological analysis was done in 
      order to study the prostaglandins induced changes in granulosa cells of all the 
      three categories of follicles. The acridine orange (AO) and methylene blue (MB) 
      staining were used to study the apoptotic index in all the three categories of 
      follicles and the collected data were analyzed by ANOVA with Duncan post Hoc 
      test. RESULTS: Prostaglandins E(2) revealed positive while PGF(2alpha) showed a 
      negative effect on the rescue of apoptosis in granulosa cells. The PGE(2) treated 
      follicles revealed a reduction in the attributes of apoptosis in granulosa cells 
      while PGF(2alpha) showed an increased in the apoptotic characteristics. CONCLUSION: 
      The present study depicted that granulosa cell viability in vitro is dependent on 
      the continuous supply of survival and growth factors and prostaglandin of E 
      series synthesis is a crucial step in the suppression of granulosa cell apoptosis 
      while that of F series induces apoptosis.
FAU - Sharma, A K
AU  - Sharma AK
AD  - Ph.D. Student in Reproductive Physiology, Reproductive Physiology Laboratory, 
      Department of Zoology, Kurukshetra University, Kurukshetra-136119, India.
FAU - Sharma, R K
AU  - Sharma RK
AD  - Reproductive Physiology Laboratory, Department of Zoology, Kurukshetra 
      University, Kurukshetra-136119, India.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Iran J Vet Res
JT  - Iranian journal of veterinary research
JID - 101660030
PMC - PMC7430371
OTO - NOTNLM
OT  - Apoptosis
OT  - Granulosa cells
OT  - Ovaries
OT  - Prostaglandins
EDAT- 2020/08/28 06:00
MHDA- 2020/08/28 06:01
PMCR- 2020/03/01
CRDT- 2020/08/28 06:00
PHST- 2020/08/28 06:00 [entrez]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2020/08/28 06:01 [medline]
PHST- 2020/03/01 00:00 [pmc-release]
AID - ijvr-21-097 [pii]
PST - ppublish
SO  - Iran J Vet Res. 2020 Spring;21(2):97-102.