PMID- 32852000
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 21
DP  - 2020 Oct 26
TI  - A DNAzyme-based label-free fluorescent probe for guanosine-5'-triphosphate 
      detection.
PG  - 6948-6954
LID - 10.1039/d0an01334j [doi]
AB  - Guanosine-5'-triphosphate (GTP) plays a key role in many important biological 
      processes of cells. It is not only a primer for DNA replication and one of the 
      four essential nucleoside triphosphates for mRNA synthesis, but also an energy 
      source for translation and other important cellular processes. It can be 
      converted to adenine nucleoside triphosphate (ATP), and the intracellular GTP 
      level is closely related to the specific pathological state, so it is crucial to 
      establish a simple and accurate method for the detection of GTP. Deoxyribozymes 
      have unique catalytic and structural properties. One of the deoxyribozymes which 
      is named DK2 with self-phosphorylation ability can transfer a phosphate from GTP 
      to the 5' end in the presence of manganese(ii), while lambda exonuclease (lambdaexo) 
      catalyzes the gradual hydrolysis of double-stranded DNA molecules phosphorylated 
      at the 5'-end from 5' to 3', but cannot cleave the 5'-OH end. The fluorescent dye 
      SYBR Green I (SG I) can bind to dsDNA and produce significant fluorescence, but 
      it can only give out weak fluorescence when it is mixed with a single strand. 
      Here, we present a novel unlabeled fluorescence assay for GTP based on the 
      self-phosphorylation of deoxyribozyme DK2 and the specific hydrolysis of lambdaexo. 
      Owing to the advantages of simple operation, high sensitivity, good specificity, 
      low cost and without fluorophore (quenching group) labeling, this method has 
      great potential in biological applications.
FAU - Hu, Chengzhen
AU  - Hu C
AD  - College of Chemistry, Green Catalysis Center, Henan Joint International Research 
      Laboratory of Green Construction of Functional Molecules and Their Bioanalytical 
      Applications, Zhengzhou University, Zhengzhou 450001, China. 
      hmmeng2017@zzu.edu.cn.
FAU - Jiang, Kemei
AU  - Jiang K
FAU - Shao, Zihao
AU  - Shao Z
FAU - Shi, Minqing
AU  - Shi M
FAU - Meng, Hong-Min
AU  - Meng HM
LA  - eng
PT  - Journal Article
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (DNA, Catalytic)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polyphosphates)
RN  - 12133JR80S (Guanosine)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - NU43IAG5BC (triphosphoric acid)
SB  - IM
MH  - *DNA, Catalytic
MH  - *Fluorescent Dyes
MH  - Guanosine
MH  - Guanosine Triphosphate
MH  - Polyphosphates
EDAT- 2020/08/28 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/08/28 06:00
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/08/28 06:00 [entrez]
AID - 10.1039/d0an01334j [doi]
PST - ppublish
SO  - Analyst. 2020 Oct 26;145(21):6948-6954. doi: 10.1039/d0an01334j.