PMID- 32852756
OWN - NLM
STAT- MEDLINE
DCOM- 20210324
LR  - 20210828
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2195
DP  - 2021
TI  - Fluorescence In Situ Hybridization (FISH) Detection of Chromosomal 12p Anomalies 
      in Testicular Germ Cell Tumors.
PG  - 49-63
LID - 10.1007/978-1-0716-0860-9_4 [doi]
AB  - Gains of genetic material or internal rearrangements of chromosome 12p, including 
      12p overrepresentation or isochromosome 12p [i(12p)], are observed in virtually 
      all germ cell tumors (GCT), in all histologic subtypes, and from various body 
      locations. The chromosomal region involved in these alterations contains the 
      growth and survival promoting oncogene KRAS (12p12.1). Gains or rearrangements of 
      12p characterize GCT from in situ to chemoresistant stages. Fluorescence in situ 
      hybridization (FISH) detection of chromosome 12p anomalies is a sensitive and 
      specific test for the diagnosis of germ cell tumors. Here we provide a detailed 
      protocol for FISH detection of isochromosome 12p and chromosome 12p 
      overrepresentation. The method is helpful for diagnosis of germ cell origin, and 
      for selection of patients who may benefit from cisplatin-based chemotherapy.
FAU - Cheng, Liang
AU  - Cheng L
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA. liang_cheng@yahoo.com.
AD  - Department of Urology, Indiana University School of Medicine, Indianapolis, IN, 
      USA. liang_cheng@yahoo.com.
FAU - Davidson, Darrell D
AU  - Davidson DD
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Montironi, Rodolfo
AU  - Montironi R
AD  - Institute of Pathological Anatomy and Histopathology, School of Medicine, 
      Polytechnic University of the Marche Region (Ancona), United Hospitals, Ancona, 
      Italy.
FAU - Wang, Mingsheng
AU  - Wang M
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
FAU - Lopez-Beltran, Antonio
AU  - Lopez-Beltran A
AD  - Department of Pathology and Surgery, Faculty of Medicine, University of Cordoba, 
      Cordoba, Spain.
FAU - Masterson, Timothy A
AU  - Masterson TA
AD  - Department of Urology, Indiana University School of Medicine, Indianapolis, IN, 
      USA.
FAU - Albany, Costantine
AU  - Albany C
AD  - Department of Medicine, Division of Hematology and Oncology, Indiana University 
      Simon Cancer Center, Indianapolis, IN, USA.
FAU - Zhang, Shaobo
AU  - Zhang S
AD  - Department of Pathology and Laboratory Medicine, Indiana University School of 
      Medicine, Indianapolis, IN, USA.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Biomarkers, Tumor)
RN  - Testicular Germ Cell Tumor
SB  - IM
MH  - Biomarkers, Tumor
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 12
MH  - Diagnosis, Differential
MH  - Genetic Association Studies
MH  - Genetic Predisposition to Disease
MH  - Humans
MH  - Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence/methods/standards
MH  - Male
MH  - Neoplasms, Germ Cell and Embryonal/*diagnosis/*genetics
MH  - Quality Control
MH  - Testicular Neoplasms/*diagnosis/*genetics
OTO - NOTNLM
OT  - Differential diagnosis
OT  - Fluorescence in situ hybridization (FISH)
OT  - Germ cell tumor
OT  - Isochromosome 12p/chromosomal 12p anomalies
OT  - Testis
EDAT- 2020/08/28 06:00
MHDA- 2021/03/25 06:00
CRDT- 2020/08/28 06:00
PHST- 2020/08/28 06:00 [entrez]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2021/03/25 06:00 [medline]
AID - 10.1007/978-1-0716-0860-9_4 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2021;2195:49-63. doi: 10.1007/978-1-0716-0860-9_4.