PMID- 32853632
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 1523-1755 (Electronic)
IS  - 0085-2538 (Linking)
VI  - 99
IP  - 1
DP  - 2021 Jan
TI  - A nonbiodegradable scaffold-free cell sheet of genome-engineered mesenchymal stem 
      cells inhibits development of acute kidney injury.
PG  - 117-133
LID - S0085-2538(20)30960-1 [pii]
LID - 10.1016/j.kint.2020.07.043 [doi]
AB  - Cell therapy using genome-engineered stem cells has emerged as a novel strategy 
      for the treatment of kidney diseases. By exploiting genome editing technology, 
      human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) secreting an 
      angiogenic factors or an anti-inflammatory factor were generated for therapeutic 
      application in acute kidney injury. Junction polymerase chain reaction analysis 
      verified zinc finger nucleases-assisted integration of the desired gene into the 
      hUC-MSCs. Flow cytometry and differentiation assays indicated that genome editing 
      did not affect the differentiation potential of these mesenchymal stem cells. 
      Protein measurement in conditioned media with the use of ELISA and immunoblotting 
      revealed the production and secretion of each integrated gene product. For cell 
      therapy in the bilateral ischemia-reperfusion mouse model of acute kidney injury, 
      our innovative scaffold-free cell sheets were established using a 
      non-biodegradable temperature-responsive polymer. One of each type of 
      scaffold-free cell sheets of either the angiogenic factor vascular endothelial 
      grown factor or angiopoietin-1, or the anti-inflammatory factor erythropoietin, 
      or alpha-melanocyte-stimulating hormone-secreting hUC-MSCs was applied to the 
      decapsulated kidney surface. This resulted in significant amelioration of kidney 
      dysfunction in the mice with acute kidney injury, effects that were superior to 
      intravenous administration of the same genome-engineered hUC-MSCs. Thus, our 
      scaffold-free cell sheets of genome-engineered mesenchymal stem cells provides 
      therapeutic effects by inhibiting acute kidney injury via angiogenesis or 
      anti-inflammation.
CI  - Copyright (c) 2020 International Society of Nephrology. Published by Elsevier Inc. 
      All rights reserved.
FAU - Park, Hye-Jeong
AU  - Park HJ
AD  - Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook 
      National University, Taegu, Korea; BK21 Plus KNU Biomedical Convergence Program, 
      Department of Biomedical Science, School of Medicine, Kyungpook National 
      University, Taegu, Korea.
FAU - Kong, Min Jung
AU  - Kong MJ
AD  - BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, 
      School of Medicine, Kyungpook National University, Taegu, Korea; Department of 
      Anatomy, School of Medicine, Kyungpook National University, Taegu, Korea.
FAU - Jang, Hyo-Ju
AU  - Jang HJ
AD  - Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook 
      National University, Taegu, Korea; BK21 Plus KNU Biomedical Convergence Program, 
      Department of Biomedical Science, School of Medicine, Kyungpook National 
      University, Taegu, Korea.
FAU - Cho, Jeong-In
AU  - Cho JI
AD  - Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook 
      National University, Taegu, Korea; BK21 Plus KNU Biomedical Convergence Program, 
      Department of Biomedical Science, School of Medicine, Kyungpook National 
      University, Taegu, Korea.
FAU - Park, Eui-Jung
AU  - Park EJ
AD  - Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook 
      National University, Taegu, Korea; BK21 Plus KNU Biomedical Convergence Program, 
      Department of Biomedical Science, School of Medicine, Kyungpook National 
      University, Taegu, Korea.
FAU - Lee, In-Kyu
AU  - Lee IK
AD  - BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, 
      School of Medicine, Kyungpook National University, Taegu, Korea; Department of 
      Internal Medicine, School of Medicine, Kyungpook National University, Taegu, 
      Korea.
FAU - Frokiaer, Jorgen
AU  - Frokiaer J
AD  - Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
FAU - Norregaard, Rikke
AU  - Norregaard R
AD  - Department of Clinical Medicine, Aarhus University, Aarhus, Denmark.
FAU - Park, Kwon Moo
AU  - Park KM
AD  - BK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, 
      School of Medicine, Kyungpook National University, Taegu, Korea; Department of 
      Anatomy, School of Medicine, Kyungpook National University, Taegu, Korea.
FAU - Kwon, Tae-Hwan
AU  - Kwon TH
AD  - Department of Biochemistry and Cell Biology, School of Medicine, Kyungpook 
      National University, Taegu, Korea; BK21 Plus KNU Biomedical Convergence Program, 
      Department of Biomedical Science, School of Medicine, Kyungpook National 
      University, Taegu, Korea. Electronic address: thkwon@knu.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200824
PL  - United States
TA  - Kidney Int
JT  - Kidney international
JID - 0323470
SB  - IM
CIN - Kidney Int. 2021 Jan;99(1):22-24. PMID: 33390229
MH  - *Acute Kidney Injury/genetics/therapy
MH  - Animals
MH  - Cell Differentiation
MH  - *Mesenchymal Stem Cell Transplantation
MH  - *Mesenchymal Stem Cells
MH  - Mice
MH  - Umbilical Cord
OTO - NOTNLM
OT  - acute kidney injury
OT  - angiogenesis
OT  - anti-inflammation
OT  - genome editing
OT  - mesenchymal stem cells
EDAT- 2020/08/28 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/08/28 06:00
PHST- 2020/02/26 00:00 [received]
PHST- 2020/07/15 00:00 [revised]
PHST- 2020/07/30 00:00 [accepted]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/08/28 06:00 [entrez]
AID - S0085-2538(20)30960-1 [pii]
AID - 10.1016/j.kint.2020.07.043 [doi]
PST - ppublish
SO  - Kidney Int. 2021 Jan;99(1):117-133. doi: 10.1016/j.kint.2020.07.043. Epub 2020 
      Aug 24.