PMID- 32853878
OWN - NLM
STAT- MEDLINE
DCOM- 20201109
LR  - 20210320
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Linking)
VI  - 126
DP  - 2020 Oct
TI  - Identification of candidate microRNAs from Ostreid herpesvirus-1 (OsHV-1) and 
      their potential role in the infection of Pacific oysters (Crassostrea gigas).
PG  - 153-164
LID - S0161-5890(20)30447-8 [pii]
LID - 10.1016/j.molimm.2020.08.004 [doi]
AB  - Oyster production is an economic activity of great interest worldwide. Recently, 
      oysters have been suffering significant mortalities from OsHV-1infection, which 
      has resulted in substantial economic loses in several countries around the world. 
      Understanding viral pathogenicity mechanisms is of central importance for the 
      establishment of disease control measures. Thus, the present work aimed to 
      identify and characterize miRNAs from OsHV-1 as well as to predict their target 
      transcripts in the virus and the host. OsHV-1 genome was used for the in silico 
      discovery of pre-miRNAs. Subsequently, viral and host target transcripts of the 
      OsHV-1 miRNAs were predicted according to the base pairing interaction between 
      mature miRNAs and mRNA 3' untranslated regions (UTRs). Six unique pre-miRNAs were 
      found in different regions of the viral genome, ranging in length from 85 to 172 
      nucleotides. A complex network of self-regulation of viral gene expression 
      mediated by the miRNAs was identified. These sequences also seem to have a broad 
      ability to regulate the expression of host immune-related genes, especially those 
      associated with pathogen recognition. Our results suggest that OsHV-1 encodes 
      miRNAs with important functions in the infection process, inducing 
      self-regulation of viral transcripts, as well as affecting the regulation of 
      Pacific oyster transcripts related to immunity. Understanding the molecular basis 
      of host-pathogen interactions can help mitigate the recurrent events of oyster 
      mass mortalities by OsHV-1 observed worldwide.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Mendes, Andrei Felix
AU  - Mendes AF
AD  - Laboratorio de Imunologia e Patologia de Invertebrados (LABIPI), Departamento de 
      Biologia Molecular, Universidade Federal da Paraiba (UFPB), 58051-900, Joao 
      Pessoa, Paraiba, Brazil.
FAU - Goncalves, Priscila
AU  - Goncalves P
AD  - Environment and Sustainability Institute, University of Exeter, Penryn, Cornwall, 
      TR10 9FE, UK.
FAU - Serrano-Solis, Victor
AU  - Serrano-Solis V
AD  - Laboratorio de Imunologia e Patologia de Invertebrados (LABIPI), Departamento de 
      Biologia Molecular, Universidade Federal da Paraiba (UFPB), 58051-900, Joao 
      Pessoa, Paraiba, Brazil.
FAU - Silva, Patricia Mirella da
AU  - Silva PMD
AD  - Laboratorio de Imunologia e Patologia de Invertebrados (LABIPI), Departamento de 
      Biologia Molecular, Universidade Federal da Paraiba (UFPB), 58051-900, Joao 
      Pessoa, Paraiba, Brazil. Electronic address: mirella_dasilva@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200824
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Viral)
RN  - Ostreid herpesvirus 1
SB  - IM
EIN - Mol Immunol. 2021 May;133:173-174. PMID: 33743265
MH  - Animals
MH  - Aquaculture
MH  - Computational Biology
MH  - Crassostrea/genetics/immunology/*virology
MH  - DNA Viruses/genetics/immunology/*pathogenicity
MH  - Gene Expression Regulation/immunology
MH  - Gene Regulatory Networks/genetics/immunology
MH  - Host-Pathogen Interactions/*genetics/immunology
MH  - MicroRNAs/genetics/isolation & purification/*metabolism
MH  - RNA, Viral/genetics/isolation & purification/*metabolism
OTO - NOTNLM
OT  - Gene expression
OT  - Mallacoherpesviridae
OT  - Viral miRNAs
OT  - ncRNAs
EDAT- 2020/08/28 06:00
MHDA- 2020/11/11 06:00
CRDT- 2020/08/28 06:00
PHST- 2020/04/14 00:00 [received]
PHST- 2020/07/31 00:00 [revised]
PHST- 2020/08/06 00:00 [accepted]
PHST- 2020/08/28 06:00 [pubmed]
PHST- 2020/11/11 06:00 [medline]
PHST- 2020/08/28 06:00 [entrez]
AID - S0161-5890(20)30447-8 [pii]
AID - 10.1016/j.molimm.2020.08.004 [doi]
PST - ppublish
SO  - Mol Immunol. 2020 Oct;126:153-164. doi: 10.1016/j.molimm.2020.08.004. Epub 2020 
      Aug 24.