PMID- 32855685
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 20
IP  - 4
DP  - 2020 Oct
TI  - Rhodiola Crenulata ameliorates exhaustive exercise-induced fatigue in mice by 
      suppressing mitophagy in skeletal muscle.
PG  - 3161-3173
LID - 10.3892/etm.2020.9072 [doi]
AB  - The aim of present study was to evaluate the potential effects of Rhodiola 
      crenulata oral liquid (RCOL) on exhaustive exercise (EE)-induced fatigue in mice. 
      Male Institute of Cancer Research mice from five treatment groups (n=10 per 
      group) were orally administered with sterilized water for the Control and EE 
      groups and/or RCOL at doses of 1.02, 3.03 and 6.06 ml/kg/day, once daily for 2 
      weeks. Anti-fatigue activity was subsequently evaluated by measuring the levels 
      of creatine kinase (CK), lactic acid (LA), lactate dehydrogenase (LDH), 
      malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and total 
      anti-oxidative capability (T-AOC). Histopathology was assessed using hematoxylin 
      and eosin staining. Ultrastructures of mitochondria were observed by transmission 
      electron microscopy. Energy supply capacity was assessed using citrate synthase 
      (CS), succinate dehydrogenase (SDH), Na(+)-K(+)-ATPase, and liver and quadriceps 
      glycogen content assays. Expression levels of mRNA and protein associated with 
      mitophagy in the skeletal muscle were measured by reverse 
      transcription-quantitative PCR and western blotting, respectively. RCOL was 
      observed to markedly inhibit fatigue-induced oxidative stress by increasing the 
      activities of SOD, CAT and T-AOC, whilst reducing the accumulation of LA, CK, LDH 
      and MDA. Histological analysis of the quadriceps femoris tissue suggested 
      increased numbers of muscle fibers in the RCOL groups compared with those in the 
      EE group. RCOL administration was found to reverse EE-induced mitochondrial 
      structural damage and alleviated defects inflicted onto the energy supply 
      mechanism by increasing CS, SDH, Na(+)-K(+)-ATPase and glycogen levels. 
      Additionally, RCOL reduced the protein expression of PTEN-induced kinase 1 
      (PINK1), Parkin, microtubule-associated proteins 1A/1B light chain 3, 
      sequestosome 1 and ubiquitin, whilst lowering the gene expression of PINK1 and 
      Parkin. Taken together, results from the present study clarified the anti-fatigue 
      effect of RCOL, where the underlying mechanism may be associated with increased 
      antioxidant activity, enhanced energy production and the inhibition of mitophagy 
      by suppressing the PINK1/Parkin signaling pathway.
CI  - Copyright: (c) Hou et al.
FAU - Hou, Ya
AU  - Hou Y
AD  - Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu 
      University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Tang, Yan
AU  - Tang Y
AD  - Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu 
      University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Wang, Xiaobo
AU  - Wang X
AD  - Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu 
      University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Ai, Xiaopeng
AU  - Ai X
AD  - Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu 
      University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Wang, Hongling
AU  - Wang H
AD  - Ethnic Medicine Academic Heritage Innovation Research Center, Chengdu University 
      of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Li, Xuanhao
AU  - Li X
AD  - Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu 
      University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Chen, Xiaorui
AU  - Chen X
AD  - Department of Pharmacology of Chinese Materia Medica, School of Pharmacy, Chengdu 
      University of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Zhang, Yi
AU  - Zhang Y
AD  - Ethnic Medicine Academic Heritage Innovation Research Center, Chengdu University 
      of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Hu, Yao
AU  - Hu Y
AD  - Interdisciplinary Laboratory of Exercise and Medicine, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Meng, Xianli
AU  - Meng X
AD  - Innovative Institute of Chinese Medicine and Pharmacy, Chengdu University of 
      Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
FAU - Zhang, Jing
AU  - Zhang J
AD  - Ethnic Medicine Academic Heritage Innovation Research Center, Chengdu University 
      of Traditional Chinese Medicine, Chengdu, Sichuan 611137, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200729
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7444336
OTO - NOTNLM
OT  - Rhodiola crenulata oral liquid
OT  - anti-fatigue
OT  - loaded swimming
OT  - mitophagy
OT  - quadriceps femoris
EDAT- 2020/08/29 06:00
MHDA- 2020/08/29 06:01
PMCR- 2020/07/29
CRDT- 2020/08/29 06:00
PHST- 2019/09/29 00:00 [received]
PHST- 2020/05/20 00:00 [accepted]
PHST- 2020/08/29 06:00 [entrez]
PHST- 2020/08/29 06:00 [pubmed]
PHST- 2020/08/29 06:01 [medline]
PHST- 2020/07/29 00:00 [pmc-release]
AID - ETM-0-0-9072 [pii]
AID - 10.3892/etm.2020.9072 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Oct;20(4):3161-3173. doi: 10.3892/etm.2020.9072. Epub 2020 Jul 
      29.