PMID- 32858006
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20210614
IS  - 1096-0945 (Electronic)
IS  - 0014-4800 (Linking)
VI  - 116
DP  - 2020 Oct
TI  - MicroRNA-29a functions as a tumor suppressor through targeting STAT3 in laryngeal 
      squamous cell carcinoma.
PG  - 104521
LID - S0014-4800(20)30797-8 [pii]
LID - 10.1016/j.yexmp.2020.104521 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a common head and neck cancer with 
      high mortality in developing countries. A comprehensive understanding of the 
      molecular mechanisms of tumor progression in laryngeal cancer is needed for new 
      treatment strategies. MicroRNA-29a has been emerged as a critical miRNA in 
      various cancers, and shown to regulate multiple oncogenic processes. In this 
      study, we investigated the tumor suppressive role of miRNA-29a in laryngeal 
      squamous cell carcinoma. We performed cell-based functional analysis after 
      overexpression of miR-29a in AMC-HN-8 and HEP2 cells in vitro. It turned out that 
      ectopic expression of miR-29a significantly inhibited cell proliferation, 
      invasion, and migration in two LSCC cell lines (AMC-HN-8 and Hep2) in vitro. 
      Further downstream target analysis by western blotting and luciferease reporter 
      assay showed that overexpression of miR-29a significantly downregulated 
      expression of STAT3, and STAT3 is a putative direct target of miR-29a in LSCC. In 
      addition, the tumor suppressive function of miR-29a can be abrogated by forced 
      expression of STAT3. Taken together, our data suggest that miR-29a functions as a 
      tumor suppressor through targeting STAT3 in laryngeal squamous cell carcinoma.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Liu, Yi-Bin
AU  - Liu YB
AD  - Department of Otolaryngology, Ganzhou People's Hospital， Ganzhou City, Jiangxi 
      Province, PR China.
FAU - Wang, Ying
AU  - Wang Y
AD  - Department of Otolaryngology, Liaocheng People's Hospital and EENT 
      Hospital,Liaocheng Clinical School of Taishan Medical University, Liaocheng, 
      Shandong, Province, PR China. Electronic address: drwangying1982@gmail.com.
FAU - Zhang, Ming-De
AU  - Zhang MD
AD  - Department of Otolaryngology, Liaocheng Dongchangfu People's Hospital, Liaocheng, 
      Shandong, Province, PR China.
FAU - Yue, Wei
AU  - Yue W
AD  - Department of Otolaryngology, Liaocheng Dongchangfu People's Hospital, Liaocheng, 
      Shandong, Province, PR China.
FAU - Sun, Chi-Nan
AU  - Sun CN
AD  - Department of Otolaryngology, Liaocheng Dongchangfu People's Hospital, Liaocheng, 
      Shandong, Province, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200826
PL  - Netherlands
TA  - Exp Mol Pathol
JT  - Experimental and molecular pathology
JID - 0370711
RN  - 0 (MIRN29a microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (STAT3 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Apoptosis/genetics
MH  - Carcinogenesis/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement/genetics
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - STAT3 Transcription Factor/*genetics
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/pathology
MH  - Tumor Suppressor Proteins/*genetics
OTO - NOTNLM
OT  - Laryngeal squamous cell carcinoma
OT  - MicroRNA-29a
OT  - STAT3
OT  - Tumor progression
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2020/08/29 06:00
MHDA- 2020/12/16 06:00
CRDT- 2020/08/29 06:00
PHST- 2020/01/25 00:00 [received]
PHST- 2020/05/28 00:00 [revised]
PHST- 2020/08/20 00:00 [accepted]
PHST- 2020/08/29 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
PHST- 2020/08/29 06:00 [entrez]
AID - S0014-4800(20)30797-8 [pii]
AID - 10.1016/j.yexmp.2020.104521 [doi]
PST - ppublish
SO  - Exp Mol Pathol. 2020 Oct;116:104521. doi: 10.1016/j.yexmp.2020.104521. Epub 2020 
      Aug 26.