PMID- 32858813
OWN - NLM
STAT- MEDLINE
DCOM- 20210219
LR  - 20210219
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Aug 26
TI  - Functional Analysis of Two Zinc (Zn) Transporters (ZIP3 and ZIP8) Promoters and 
      Their Distinct Response to MTF1 and RREB1 in the Regulation of Zn Metabolism.
LID - 10.3390/ijms21176135 [doi]
LID - 6135
AB  - ZIP (zinc-regulated transporters, iron-regulated transporter-like protein) family 
      plays an important role in organism Zn balance. This research identified the 
      promoter regions of ZIP3 and ZIP8, two members of ZIP family, from a freshwater 
      teleost yellow catfish Pelteobagrus fulvidraco, characterized the binding 
      sequences of the metal-responsive transcription factor-1 (MTF-1) and Ras 
      responsive element binding protein 1 (RREB1) on their promoter regions. The 
      present study cloned and obtained the 2027 bp of ZIP3 promoter and 1664 bp of 
      ZIP8 promoter, and predicted several key elements on their promoters, such as the 
      binding sites of CREB (cAMP-response element binding protein), KLF4 (Kruppel like 
      factor 4), MTF-1 and RREB1. The sequence deletion from -361 bp to -895 bp 
      down-regulated the luciferase activity of ZIP3 promoter, and the deletion from 
      -897 bp to -1664 bp down-regulated the luciferase activity of ZIP8 promoter. 
      Within different deletion plasmids, the relative luciferase activities of ZIP3 
      and ZIP8 promoters changes to Zn incubation in a Zn concentration-dependent 
      manner. The site mutagenesis and EMSA (electrophoretic mobility shift assay) 
      found that the -1327 bp/-1343 bp MTF-1 binding site and the -248 bp/-267 bp RREB1 
      binding site on the ZIP3 promoter, and the -1543 bp/-1557 bp MTF-1 binding site 
      on the ZIP8 promoter are functional sites. Low Zn increased the binding 
      capability between MTF-1 and its responsive site on the ZIP3 promoter, and high 
      Zn increased the transcriptional activation ZIP3 by RREB1; Zn also promoted the 
      binding ability between MTF-1 and its responsive element on the ZIP8 promoter. 
      This study provides the first direct evidence for the response elements of MTF-1 
      and RREB1 on ZIP3 and MTF-1 on ZIP8 to Zn, which are very important for the 
      evaluation of Zn nutrition and toxicity in vertebrates.
FAU - Chen, Shu-Wei
AU  - Chen SW
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery 
      College, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Wu, Kun
AU  - Wu K
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery 
      College, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Lv, Wu-Hong
AU  - Lv WH
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery 
      College, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Chen, Fang
AU  - Chen F
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery 
      College, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Song, Chang-Chun
AU  - Song CC
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery 
      College, Huazhong Agricultural University, Wuhan 430070, China.
FAU - Luo, Zhi
AU  - Luo Z
AUID- ORCID: 0000-0003-3219-1609
AD  - Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture, Fishery 
      College, Huazhong Agricultural University, Wuhan 430070, China.
AD  - Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao 
      National Laboratory for Marine Science and Technology, 1 Wenhai Road, Qingdao 
      266237, China.
LA  - eng
GR  - 31872585/National Natural Science Foundation of China/
GR  - 2018YFD0900400/National Key R&D Program of China/
PT  - Journal Article
DEP - 20200826
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Fish Proteins)
RN  - 0 (Transcription Factors)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Catfishes/*genetics/metabolism
MH  - Cation Transport Proteins/chemistry/*genetics
MH  - Cloning, Molecular
MH  - Fish Proteins/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Promoter Regions, Genetic
MH  - Sequence Deletion
MH  - Transcription Factors/*metabolism
MH  - Zinc/*metabolism
PMC - PMC7503416
OTO - NOTNLM
OT  - ZIP transporter
OT  - Zn homeostasis
OT  - Zn toxicity
OT  - fish
OT  - transcriptional regulation
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/08/30 06:00
MHDA- 2021/02/20 06:00
PMCR- 2020/09/01
CRDT- 2020/08/30 06:00
PHST- 2020/07/27 00:00 [received]
PHST- 2020/08/13 00:00 [revised]
PHST- 2020/08/15 00:00 [accepted]
PHST- 2020/08/30 06:00 [entrez]
PHST- 2020/08/30 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21176135 [pii]
AID - ijms-21-06135 [pii]
AID - 10.3390/ijms21176135 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Aug 26;21(17):6135. doi: 10.3390/ijms21176135.