PMID- 32858886
OWN - NLM
STAT- MEDLINE
DCOM- 20210219
LR  - 20210219
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Aug 26
TI  - Conventional Co-Housing Modulates Murine Gut Microbiota and Hematopoietic Gene 
      Expression.
LID - 10.3390/ijms21176143 [doi]
LID - 6143
AB  - Specific-pathogen-free (SPF) mice have improved hematopoietic characteristics 
      relative to germ-free mice, however, it is not clear whether improvements in 
      hematopoietic traits will continue when the level of microorganism exposure is 
      further increased. We co-housed SPF C57BL/6 mice in a conventional facility (CVT) 
      and found a significant increase in gut microbiota diversity along with increased 
      levels of myeloid cells and T cells, especially effector memory T cells. Through 
      single cell RNA sequencing of sorted KL (c-Kit(+)Lin(-)) cells, we imputed a 
      decline in long-term hematopoietic stem cells and an increase in 
      granulocyte-monocyte progenitors in CVT mice with up-regulation of genes 
      associated with cell survival. Bone marrow transplantation through competitive 
      repopulation revealed a significant increase in KSL (c-Kit(+)Sca-1(+)Lin(-)) cell 
      reconstitution in recipients of CVT donor cells which occurred when donors were 
      co-housed for both one and twelve months. However, there was minimal to no gain 
      in mature blood cell engraftment in recipients of CVT donor cells relative to 
      those receiving SPF donor cells. We conclude that co-housing SPF mice with mice 
      born in a conventional facility increased gut microbiota diversity, augmented 
      myeloid cell production and T cell activation, stimulated KSL cell 
      reconstitution, and altered hematopoietic gene expression.
FAU - Chen, Jichun
AU  - Chen J
AD  - Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Zhang, Shuling
AU  - Zhang S
AD  - Laboratory of Cancer Biology and Genetics, National Cancer Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA.
FAU - Feng, Xingmin
AU  - Feng X
AD  - Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Wu, Zhijie
AU  - Wu Z
AD  - Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Dubois, Wendy
AU  - Dubois W
AD  - Laboratory of Cancer Biology and Genetics, National Cancer Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA.
FAU - Thovarai, Vishal
AU  - Thovarai V
AD  - Basic Science Program, Frederick National Laboratory for Cancer Research, 
      Frederick, MD 21702, USA.
FAU - Ahluwalia, Sonia
AU  - Ahluwalia S
AD  - Basic Science Program, Frederick National Laboratory for Cancer Research, 
      Frederick, MD 21702, USA.
FAU - Gao, Shouguo
AU  - Gao S
AD  - Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Chen, Jinguo
AU  - Chen J
AD  - Center for Human Immunology and Autoimmunity, National Institutes of Health, 
      Bethesda, MD 20892, USA.
FAU - Peat, Tyler
AU  - Peat T
AD  - Laboratory of Cancer Biology and Genetics, National Cancer Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA.
FAU - Sen, Shurjo K
AU  - Sen SK
AD  - Basic Science Program, Frederick National Laboratory for Cancer Research, 
      Frederick, MD 21702, USA.
FAU - Trinchieri, Giorgio
AU  - Trinchieri G
AD  - Cancer and Inflammation Program, Center for Cancer Research, National Cancer 
      Institute, National Institutes of Health, Bethesda, MD 20892, USA.
FAU - Young, Neal S
AU  - Young NS
AD  - Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes 
      of Health, Bethesda, MD 20892, USA.
FAU - Mock, Beverly A
AU  - Mock BA
AD  - Laboratory of Cancer Biology and Genetics, National Cancer Institute, National 
      Institutes of Health, Bethesda, MD 20892, USA.
LA  - eng
GR  - NHLBI Intramural Research Program/NH/NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
DEP - 20200826
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Animals
MH  - Bacteria/*classification/genetics/isolation & purification
MH  - Bone Marrow Transplantation
MH  - Gastrointestinal Microbiome
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation
MH  - *Hematopoiesis
MH  - Housing, Animal
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myeloid Cells/*metabolism
MH  - Phylogeny
MH  - Sequence Analysis, RNA/*methods
MH  - Single-Cell Analysis
MH  - Specific Pathogen-Free Organisms
MH  - T-Lymphocytes/*metabolism
PMC - PMC7503692
OTO - NOTNLM
OT  - conventional co-housing
OT  - gut microbiota
OT  - hematopoietic stem and progenitor cells
OT  - mice
OT  - single cell RNA-seq
OT  - specific pathogen free
COIS- The authors declare no competing financial interests.
EDAT- 2020/08/30 06:00
MHDA- 2021/02/20 06:00
PMCR- 2020/09/01
CRDT- 2020/08/30 06:00
PHST- 2020/07/28 00:00 [received]
PHST- 2020/08/21 00:00 [revised]
PHST- 2020/08/21 00:00 [accepted]
PHST- 2020/08/30 06:00 [entrez]
PHST- 2020/08/30 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21176143 [pii]
AID - ijms-21-06143 [pii]
AID - 10.3390/ijms21176143 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Aug 26;21(17):6143. doi: 10.3390/ijms21176143.