PMID- 32862176
OWN - NLM
STAT- MEDLINE
DCOM- 20210806
LR  - 20240226
IS  - 1421-9735 (Electronic)
IS  - 0253-5068 (Linking)
VI  - 50
IP  - 2
DP  - 2021
TI  - Associations between Cell-Free Mitochondrial DNA and Inflammation, and Their 
      Clinical Implications for Patients on Hemodialysis: A Prospective Multicenter 
      Cohort Study.
PG  - 214-221
LID - 10.1159/000510088 [doi]
AB  - BACKGROUND: Cell-free mitochondrial DNA (cf-mtDNA) has recently been in the 
      spotlight as an endogenously produced danger molecule that can potentially elicit 
      inflammation. However, its clinical and prognostic implications are uncertain in 
      patients undergoing hemodialysis. METHODS: We examined the association of 
      baseline cf-mtDNA categorized as tertiles with health-related quality of life 
      (HRQOL), inflammatory cytokines, and mortality in a multicenter prospective 
      cohort of 334 patients on hemodialysis. To better understand cf-mtDNA-mediated 
      inflammation, we measured cytokine production after in vitro stimulation of bone 
      marrow-derived macrophages (BMDMs) with mtDNA. RESULTS: The higher cf-mtDNA 
      tertile had a longer dialysis vintage, a greater comorbidity burden, and 
      increased levels of inflammatory markers, including high-sensitivity-C-reactive 
      protein, tumor necrosis factor-alpha, CXCL16, and osteoprotegerin. In particular, 
      mtDNA augmented inflammatory cytokine release from BMDMs by lipopolysaccharide, 
      the levels of which are reported to be increased in hemodialysis patients. 
      Although the patients with higher levels of cf-mtDNA generally had lower (poorer) 
      scores for HRQOL, cf-mtDNA was not associated with all-cause mortality in 
      hemodialysis patients. CONCLUSION: cf-mtDNA was correlated with poor clinical 
      status and modestly associated with impaired quality of life in patients on 
      hemodialysis. In proinflammatory milieu in end-stage renal disease, these 
      associations may be attributed to the boosting effects of cf-mtDNA on 
      inflammation.
CI  - (c) 2020 S. Karger AG, Basel.
FAU - Kim, Kipyo
AU  - Kim K
AD  - Division of Nephrology and Hypertension, Department of Internal Medicine, Inha 
      University, Incheon, Republic of Korea.
FAU - Jung, Su Woong
AU  - Jung SW
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea.
FAU - Cho, Won-Hee
AU  - Cho WH
AD  - Department of Medicine, Graduate School, Kyung Hee University, Seoul, Republic of 
      Korea.
FAU - Moon, Haena
AU  - Moon H
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea.
FAU - Jeong, Kyung Hwan
AU  - Jeong KH
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea.
FAU - Kim, Jin Sug
AU  - Kim JS
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea.
FAU - Lee, Sang-Ho
AU  - Lee SH
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea.
FAU - Ahn, Shin Young
AU  - Ahn SY
AD  - Division of Nephrology, Department of Internal Medicine, Korea University College 
      of Medicine, Seoul, Republic of Korea.
FAU - Yang, Dong Ho
AU  - Yang DH
AD  - Division of Nephrology, Department of Internal Medicine, CHA Bundang Medical 
      Center, CHA University, Seongnam, Republic of Korea.
FAU - Lee, Hong Joo
AU  - Lee HJ
AD  - Division of Nephrology, Department of Internal Medicine, Seoul Red Cross 
      Hospital, Seoul, Republic of Korea.
FAU - Lee, Dong-Young
AU  - Lee DY
AD  - Division of Nephrology, Department of Internal Medicine, Veterans Healthcare 
      System Medical Center, Seoul, Republic of Korea.
FAU - Moon, Ju-Young
AU  - Moon JY
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea.
FAU - Kim, Yang Gyun
AU  - Kim YG
AD  - Division of Nephrology, Department of Internal Medicine, Kyung Hee University 
      School of Medicine, Seoul, Republic of Korea, apple8840@hanmail.net.
LA  - eng
PT  - Journal Article
PT  - Multicenter Study
PT  - Research Support, Non-U.S. Gov't
DEP - 20200828
PL  - Switzerland
TA  - Blood Purif
JT  - Blood purification
JID - 8402040
RN  - 0 (Cell-Free Nucleic Acids)
RN  - 0 (Cytokines)
RN  - 0 (DNA, Mitochondrial)
SB  - IM
MH  - Aged
MH  - Animals
MH  - Cell-Free Nucleic Acids/*blood/metabolism
MH  - Cells, Cultured
MH  - Cytokines/blood/metabolism
MH  - DNA, Mitochondrial/*blood/metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/*blood/metabolism
MH  - Kidney Failure, Chronic/blood/metabolism/therapy
MH  - Macrophages/metabolism
MH  - Male
MH  - Mice, Inbred C57BL
MH  - Middle Aged
MH  - Prospective Studies
MH  - *Renal Dialysis
MH  - Mice
OTO - NOTNLM
OT  - Cell-free mitochondrial DNA
OT  - Hemodialysis
OT  - Inflammation
OT  - Quality of life
EDAT- 2020/08/31 06:00
MHDA- 2021/08/07 06:00
CRDT- 2020/08/31 06:00
PHST- 2020/04/26 00:00 [received]
PHST- 2020/07/09 00:00 [accepted]
PHST- 2020/08/31 06:00 [pubmed]
PHST- 2021/08/07 06:00 [medline]
PHST- 2020/08/31 06:00 [entrez]
AID - 000510088 [pii]
AID - 10.1159/000510088 [doi]
PST - ppublish
SO  - Blood Purif. 2021;50(2):214-221. doi: 10.1159/000510088. Epub 2020 Aug 28.