PMID- 32862580
OWN - NLM
STAT- MEDLINE
DCOM- 20210210
LR  - 20231213
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 3
DP  - 2020 May-Jun
TI  - Detailed studies on the anticancer action of rosmarinic acid in human Hep-G2 
      liver carcinoma cells: evaluating its effects on cellular apoptosis, caspase 
      activation and suppression of cell migration and invasion.
PG  - 1383-1389
AB  - PURPOSE: Liver cancer is one of the most common and highly malignant cancers of 
      the digestive system. The main aim of the present research work was to 
      investigate the anticancer action of rosmarinic acid - a naturally occurring 
      plant secondary metabolite. We also investigated its effects on cell apoptosis, 
      caspase activation, cell migration and cell invasion. METHODS: Cell viability of 
      Hep-G2 liver cancer cells was evaluated by CCK-8 assay while apoptotic studies 
      were carried out by fluorescence microscopy using Hoechst, acridine orange 
      (AO)/ethidium bromide (EB) and Comet assays as well as using annexin-v/propidium 
      iodide (PI) assay for apoptosis quantification. Western blot assay was used to 
      study the effects of rosmarinic acid on apoptosis-related protein expressions 
      including Bax, Bcl-2 and various caspases. In vitro wound healing assay was used 
      to evaluate the effects on cell migration while transwell chambers assay with 
      Matrigel was used to assess the effects of rosmarinic acid on cell invasion. 
      RESULTS: Rosmarinic acid caused significant reduction in the viability of the 
      human Hep-G2 liver carcinoma cells in a dose-dependent manner, exhibiting an IC50 
      of 14 microM in cancer cells. The AO/EB staining assay showed that rosmarinic acid 
      suppressed the viability of cancer cells via induction of apoptotic cell death 
      which was associated with rise in Bax and decrease in Bcl-2 levels. DAPI staining 
      results also confirmed that rosmarinic acid induced apoptosis. The apoptotic 
      cells increased from 5.8% in control to 24.68% at 28 microM concentration of 
      rosmarinic acid. Rosmarinic acid also caused activation of caspase-3 and 9 along 
      with suppressing liver cancer cell migration and invasion. CONCLUSIONS: The 
      current study shows that rosmarinic acid has a potential to inhibit in vitro 
      cancer cell growth in Hep-G2 cells by triggering apoptosis, caspase activation 
      and suppressing cell migration and invasion and as such this molecule could be 
      developed as a possible anticancer agent provided further studies are carried 
      out.
FAU - Jin, Boxun
AU  - Jin B
AD  - Department of General Surgery, Beijing You'an Hospital, Capital Medical 
      University, Beijing, China, 100069.
FAU - Liu, Jinning
AU  - Liu J
FAU - Gao, Daming
AU  - Gao D
FAU - Xu, Ying
AU  - Xu Y
FAU - He, Li
AU  - He L
FAU - Zang, Yunjin
AU  - Zang Y
FAU - Li, Ning
AU  - Li N
FAU - Lin, Dongdong
AU  - Lin D
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cinnamates)
RN  - 0 (Depsides)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Carcinoma, Hepatocellular/*drug therapy/metabolism
MH  - Caspases/*metabolism
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cinnamates/*pharmacology
MH  - Depsides/*pharmacology
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver/drug effects/metabolism
MH  - Liver Neoplasms/*drug therapy/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Signal Transduction/drug effects
MH  - Rosmarinic Acid
EDAT- 2020/08/31 06:00
MHDA- 2021/02/11 06:00
CRDT- 2020/08/31 06:00
PHST- 2020/08/31 06:00 [entrez]
PHST- 2020/08/31 06:00 [pubmed]
PHST- 2021/02/11 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 May-Jun;25(3):1383-1389.