PMID- 32862589
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 3
DP  - 2020 May-Jun
TI  - Norwogonin flavone suppresses the growth of human colon cancer cells via 
      mitochondrial mediated apoptosis, autophagy induction and triggering G2/M phase 
      cell cycle arrest.
PG  - 1449-1454
AB  - PURPOSE: Colorectal cancer is one of the deadly malignancies and is one of the 
      top three most common cancers and the third leading cause of cancer-related 
      deaths. The main objective of the study was to investigate the anticancer effects 
      of norwogonin - a naturally occurring plant flavone. We also examined its effects 
      on programmed cell death, autophagy and cell cycle phase distribution. METHODS: 
      Cell viability of colon cancer cells was evaluated by MTT assay while apoptotic 
      studies were carried out by fluorescence microscopy using acridine orange 
      (AO)/ethidium bromide (EB) and Comet assays. Transmission electron microscopy 
      (TEM) was used to study formation of autophagosomes reminiscent of autophagy. 
      Furthermore, western blot assay was used to study the effects of norwogonin on 
      apoptosis-related protein expressions including Bax, Bcl-2 and autophagy-related 
      proteins. Effects on cell cycle were evaluated by flow cytometry. RESULTS: The 
      results showed that norwogonin causes substantial reduction in the viability of 
      the human colorectal carcinoma cells in a dose-dependent manner, exhibiting an 
      IC50 of 15.5 microM in cancer cells and IC50 of 90 microM in normal cell lines. The AO/EB 
      staining assay showed that norwogonin suppresses the viability of cancer cells 
      via induction of apoptotic cell death which was associated with increase in Bax 
      and decrease in Bcl-2 levels. Comet assay results also confirmed that norwogonin 
      induces apoptosis. Norwogonin also led to induction of autophagy along with 
      triggering G2/M phase cell cycle arrest. CONCLUSIONS: In conclusion, the current 
      study shows that norwogonin has a potential to inhibit in vitro colorectal cancer 
      cells growth by triggering apoptosis, autophagy and cell cycle arrest and as such 
      could be developed as a possible anticancer agent.
FAU - Wang, Zhang
AU  - Wang Z
AD  - Department of Gastrointestinal Surgery, the Central Hospital of Enshi Tujia and 
      Miao Autonomous Prefecture, Enshi, Hubei 445000, China.
FAU - Zhang, Qinqin
AU  - Zhang Q
FAU - Zhou, Ling
AU  - Zhou L
FAU - Liu, Gao
AU  - Liu G
FAU - Wu, Quanfeng
AU  - Wu Q
FAU - Chen, Changwang
AU  - Chen C
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Flavones)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 4443-09-8 (norwogonin)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Autophagy/*drug effects
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Colonic Neoplasms/*drug therapy/metabolism
MH  - Flavones/*pharmacology
MH  - G2 Phase Cell Cycle Checkpoints/drug effects
MH  - Humans
MH  - M Phase Cell Cycle Checkpoints/drug effects
MH  - Mitochondria/*drug effects
MH  - Proto-Oncogene Proteins c-bcl-2/metabolism
MH  - Signal Transduction/drug effects
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2020/08/31 06:00
MHDA- 2021/02/10 06:00
CRDT- 2020/08/31 06:00
PHST- 2020/08/31 06:00 [entrez]
PHST- 2020/08/31 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 May-Jun;25(3):1449-1454.