PMID- 32862602
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 3
DP  - 2020 May-Jun
TI  - UV irradiation induces apoptosis in the human endometrial stromal cell line 
      (ThESC).
PG  - 1541-1546
AB  - PURPOSE: The treatment options of endometrial hyperplasia consist of surgical, 
      interventional and medical therapies including apoptosis-inducing agents. The 
      purpose of the study was to evaluate the effects of ultraviolet (UV) radiation on 
      the viability and the type of cell death on the human endometrial stromal cells 
      (ThESC) line. METHODS: We investigated the effect of UV exposure on human 
      endometrial stromal cell line (ThESC) on cell viability using MTT assay as well 
      as changes in cell morphology using phase microscopy and acridine orange 
      (AO)/ethidium bromide (EB) cell staining. RESULTS: UV treatment significantly 
      decreased the percentage of the viable ThESC cells compared to the viability of 
      untreated control cells using MTT assay (p<0.05). In addition, UV treatment of 
      ThESC cells for 60 and 90 min induced high level of cell morphology disruption, 
      followed with loss of both the cell shape and the presence of defragmented debris 
      and stained with intense red color. CONCLUSIONS: The obtained results suggest the 
      potential role of UV light application as additional treatment option of benign 
      endometrium hyperplasia alone or in combination with other treatment modalities.
FAU - Stanojevic-Pirkovic, Marijana
AU  - Stanojevic-Pirkovic M
AD  - University of Kragujevac, Faculty of Medical Sciences, Department of 
      Biochemistry, Serbia.
FAU - Andelkovic, Marija
AU  - Andelkovic M
FAU - Lukovic, Jovan
AU  - Lukovic J
FAU - Zelen, Ivanka
AU  - Zelen I
FAU - Milosavljevic, Zoran
AU  - Milosavljevic Z
FAU - Mitrovic, Marija
AU  - Mitrovic M
FAU - Jurisic, Vladimir
AU  - Jurisic V
FAU - Nikolic, Ivana
AU  - Nikolic I
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
SB  - IM
MH  - Apoptosis/*radiation effects
MH  - Cell Death/radiation effects
MH  - Cell Line
MH  - Cell Survival/radiation effects
MH  - Endometrial Hyperplasia/*radiotherapy
MH  - Endometrium/radiation effects
MH  - Female
MH  - Humans
MH  - Stromal Cells/*radiation effects
MH  - Ultraviolet Rays
EDAT- 2020/08/31 06:00
MHDA- 2021/02/10 06:00
CRDT- 2020/08/31 06:00
PHST- 2020/08/31 06:00 [entrez]
PHST- 2020/08/31 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 May-Jun;25(3):1541-1546.