PMID- 32862616
OWN - NLM
STAT- MEDLINE
DCOM- 20210209
LR  - 20210209
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 3
DP  - 2020 May-Jun
TI  - Research on mechanism of miR-130a in regulating autophagy of bladder cancer cells 
      through CYLD.
PG  - 1636-1642
AB  - PURPOSE: The study aimed to explore the regulatory mechanism of micro ribonucleic 
      acid (miR)-130a in the autophagy of bladder cancer cells through cylindromatosis 
      (CYLD). METHODS: Human bladder cancer T24 cell line was used as the objects of 
      the study. After miR-130a was knocked down using small-interfering RNA (siRNA) in 
      T24 cell line, the changes in expressions of miR-130a and CYLD in each group were 
      detected via quantitative reverse transcription-polymerase chain reaction 
      (qRT-PCR). The cell proliferation in each group was detected using cell counting 
      kit-8 (CCK8) assay and flow cytometry. The changes in mRNA and protein levels of 
      microtubule-associated protein 1 light chain 3 (LC3) and Beclin1 were determined 
      using qRT-PCR and Western blotting. The autolysosomes were detected through 
      acridine orange (AO)/ethidium staining bromide (ER) staining. Moreover, CYLD was 
      knocked down using siRNA, and then the changes in mRNA expressions of miR-130a, 
      LC3 and Beclin1 in each group were detected through qRT-PCR. RESULTS: After 
      interference in miR-130a with siRNA, miR-130a-siRNA group had a significantly 
      lower mRNA expression of miR-130a compared with NC-siRNA group and a 
      significantly higher mRNA expression of CYLD (p<0.05), obviously inhibited cell 
      proliferation (p<0.05), and decreased significantly mRNA and protein expressions 
      of LC3 showing Beclin1 (p<0.05), and an evidently smaller number of 
      autolysosomes. After knockdown of CYLD using siRNA, the mRNA expression of 
      miR-130a had no significant changes (p>0.05), while the mRNA expressions of LC3 
      and Beclin1 declined significantly in CYLD-siRNA group compared with those in 
      NC-siRNA group (p<0.05). CONCLUSION: MiR-130 can promote the autophagy of bladder 
      cancer cells through regulating CYLD, thus facilitating the proliferation of 
      tumor cells.
FAU - Dai, Haitao
AU  - Dai H
AD  - Department of Urology, the first People's Hospital of Jingzhou (The First 
      Affiliated Hospital of Yangtze University), Jingzhou, Hubei 434000, P.R. China.
FAU - Liu, Changmao
AU  - Liu C
FAU - Liu, Yuanhua
AU  - Liu Y
FAU - Zhang, Zhong
AU  - Zhang Z
FAU - Peng, Changwei
AU  - Peng C
FAU - Wang, Zhongyu
AU  - Wang Z
FAU - Zheng, Jiang
AU  - Zheng J
FAU - Li, Chenglong
AU  - Li C
FAU - Yu, Weimin
AU  - Yu W
FAU - Cheng, Fan
AU  - Cheng F
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (MIRN130 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.4.19.12 (CYLD protein, human)
RN  - EC 3.4.19.12 (Deubiquitinating Enzyme CYLD)
SB  - IM
MH  - Apoptosis/genetics
MH  - Autophagy/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Deubiquitinating Enzyme CYLD/*genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - RNA, Messenger/genetics
MH  - RNA, Small Interfering/genetics
MH  - Urinary Bladder Neoplasms/*genetics/pathology
EDAT- 2020/08/31 06:00
MHDA- 2021/02/10 06:00
CRDT- 2020/08/31 06:00
PHST- 2020/08/31 06:00 [entrez]
PHST- 2020/08/31 06:00 [pubmed]
PHST- 2021/02/10 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 May-Jun;25(3):1636-1642.