PMID- 32863772
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 20
DP  - 2020
TI  - MicroRNA-214-3p inhibits the stem-like properties of lung squamous cell cancer by 
      targeting YAP1.
PG  - 413
LID - 10.1186/s12935-020-01506-2 [doi]
LID - 413
AB  - BACKGROUND: Emerging evidence reveals that microRNAs (miRNAs) play a crucial role 
      in tumor progression, but the underlying mechanism of microRNAs in lung squamous 
      cell cancer (LSCC) remains unclear. METHOD: Western-blotting and quantitative 
      real-time PCR (q-PCR) were carried out to detect mRNA and protein expression. 
      Cell proliferation was evaluated by Cell Counting Kit-8 (CCK-8), colony-forming 
      assay or sphere-forming assay, respectively. RESULTS: MiR-214-3p was markedly 
      de-regulated in LSCC tissues and was inversely related to the level of 
      Yes-associated protein1 (YAP1), which is the core transcription regulator of the 
      Hippo signaling pathway. Kaplan-Meier survival curves illustrated that patients 
      with high miR-214-3p expression demonstrated more favorable clinical outcomes. 
      MiR-214-3p overexpression (OE) repressed proliferation and cancer stem-like cells 
      (CSCs) properties in vitro and in vivo xenograft mouse model. Mechanistically, 
      luciferase activity assay revealed that miR-214-3p directly targets YAP1 by 
      specifically binding on the 3' UTR of YAP1. CONCLUSION: MiR-214-3p plays a 
      pivotal role in CSCs properties by targeting YAP1, which provides a potential 
      treatment strategy for LSCC patients.
CI  - (c) The Author(s) 2020.
FAU - Lu, Tingting
AU  - Lu T
AD  - Department of Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao 
      Tong University, 241 Huaihai West Road, Shanghai, 200030 People's Republic of 
      China. GRID: grid.16821.3c. ISNI: 0000 0004 0368 8293
FAU - Yang, Ying
AU  - Yang Y
AD  - Department of Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao 
      Tong University, 241 Huaihai West Road, Shanghai, 200030 People's Republic of 
      China. GRID: grid.16821.3c. ISNI: 0000 0004 0368 8293
FAU - Li, Ziming
AU  - Li Z
AD  - Department of Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao 
      Tong University, 241 Huaihai West Road, Shanghai, 200030 People's Republic of 
      China. GRID: grid.16821.3c. ISNI: 0000 0004 0368 8293
FAU - Lu, Shun
AU  - Lu S
AUID- ORCID: 0000-0003-3887-4320
AD  - Department of Shanghai Lung Cancer Center, Shanghai Chest Hospital, Shanghai Jiao 
      Tong University, 241 Huaihai West Road, Shanghai, 200030 People's Republic of 
      China. GRID: grid.16821.3c. ISNI: 0000 0004 0368 8293
LA  - eng
PT  - Journal Article
DEP - 20200827
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
PMC - PMC7450582
OTO - NOTNLM
OT  - CSC properties
OT  - MiR-214-3p
OT  - YAP1
COIS- Competing interestsThe authors declare that they have no competing interests.
EDAT- 2020/08/31 06:00
MHDA- 2020/08/31 06:01
PMCR- 2020/08/27
CRDT- 2020/09/01 06:00
PHST- 2020/05/11 00:00 [received]
PHST- 2020/08/18 00:00 [accepted]
PHST- 2020/09/01 06:00 [entrez]
PHST- 2020/08/31 06:00 [pubmed]
PHST- 2020/08/31 06:01 [medline]
PHST- 2020/08/27 00:00 [pmc-release]
AID - 1506 [pii]
AID - 10.1186/s12935-020-01506-2 [doi]
PST - epublish
SO  - Cancer Cell Int. 2020 Aug 27;20:413. doi: 10.1186/s12935-020-01506-2. eCollection 
      2020.