PMID- 32865449
OWN - NLM
STAT- MEDLINE
DCOM- 20210820
LR  - 20210820
IS  - 1557-7465 (Electronic)
IS  - 1079-9907 (Linking)
VI  - 40
IP  - 10
DP  - 2020 Oct
TI  - Spherical Gold Nanoparticles: Small Interfering RNA Delivery in Regulation of the 
      Tumor Necrosis Factor-Alpha Gene Expression.
PG  - 490-496
LID - 10.1089/jir.2020.0090 [doi]
AB  - Proinflammatory cytokines are signaling molecules that are expelled from immune 
      cells like macrophages and other types of cells. Tumor necrosis factor-alpha 
      (TNF-alpha) is overexpressed during inflammation caused by inflammatory diseases. 
      Therefore, the regulation of TNF-alpha has a key role in inflammation. The use and 
      target delivery of small interfering RNAs (siRNAs) provide many effectual 
      treatment benefits in the regulation of gene expression in cells. In this study, 
      we used siRNA nanoparticle conjugates in the regulation of gene expression and 
      inflammation. We first prepared safe fusion ribonucleic acid interference 
      carrier, spherical nucleic acid nanoparticle conjugates (SNA-NCs), to enhance the 
      perforation of siRNA into the macrophages and their ability to target TNF-alpha gene 
      regulation. Furthermore, the suppression of the TNF-alpha gene was monitored after 
      curing macrophages by SNA-NCs. Gene expression was carried out by real-time 
      polymerase chain reaction in cells and the levels of TNF-alpha were investigated by 
      the enzyme-linked immunosorbent assay (ELISA) method. This study indicated that 
      the SNA-NCs were safe and very stable. TNF-alpha siRNA could significantly regulate 
      gene expression in cells to form SNA-NCs. The results indicated that TNF-alpha gene 
      expression downregulated to 93.40% +/- 1.45%, 66.06% +/- 0.95%, and 35.76% +/- 1.09% in 
      the presence of 0.1, 1, and 10 nM siRNA, respectively. The proliferation of 
      macrophages and subsequently expression of TNF-alpha were significant for the 
      formation of inflammation. These findings showed that the use of SNA-NC siRNA 
      might ameliorate the inflammatory disease by suppression of gene expression and 
      functional activity of macrophage generation.
FAU - Hosseinzadeh, Leila
AU  - Hosseinzadeh L
AD  - Pharmaceutical Sciences Research Center, School of Pharmacy, Kermanshah 
      University of Medical Sciences, Kermanshah, Iran.
FAU - Nemati, Houshang
AU  - Nemati H
AD  - Fertility and Infertility Research Center, Health Technology Institute, 
      Kermanshah University of Medical Sciences, Kermanshah, Iran.
FAU - Nemati, Narges
AU  - Nemati N
AD  - Fertility and Infertility Research Center, Health Technology Institute, 
      Kermanshah University of Medical Sciences, Kermanshah, Iran.
FAU - Sadeghi, Masoud
AU  - Sadeghi M
AD  - Medical Biology Research Center, Kermanshah University of Medical Sciences, 
      Kermanshah, Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200828
PL  - United States
TA  - J Interferon Cytokine Res
JT  - Journal of interferon & cytokine research : the official journal of the 
      International Society for Interferon and Cytokine Research
JID - 9507088
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Cell Survival
MH  - Down-Regulation
MH  - *Gene Expression Regulation
MH  - *Gold
MH  - *Metal Nanoparticles
MH  - Mice
MH  - RAW 264.7 Cells
MH  - *RNA, Small Interfering/administration & dosage
MH  - Tumor Necrosis Factor-alpha/biosynthesis/*genetics
OTO - NOTNLM
OT  - TNF-alpha
OT  - inflammation
OT  - macrophages
OT  - nanoparticles
OT  - siRNA
EDAT- 2020/09/01 06:00
MHDA- 2021/08/21 06:00
CRDT- 2020/09/01 06:00
PHST- 2020/09/01 06:00 [pubmed]
PHST- 2021/08/21 06:00 [medline]
PHST- 2020/09/01 06:00 [entrez]
AID - 10.1089/jir.2020.0090 [doi]
PST - ppublish
SO  - J Interferon Cytokine Res. 2020 Oct;40(10):490-496. doi: 10.1089/jir.2020.0090. 
      Epub 2020 Aug 28.