PMID- 32867213
OWN - NLM
STAT- MEDLINE
DCOM- 20210222
LR  - 20210222
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Aug 27
TI  - Mitoquinone (MitoQ) Inhibits Platelet Activation Steps by Reducing ROS Levels.
LID - 10.3390/ijms21176192 [doi]
LID - 6192
AB  - Platelet activation plays a key role in cardiovascular diseases. The generation 
      of mitochondrial reactive oxygen species (ROS) has been described as a critical 
      step required for platelet activation. For this reason, it is necessary to find 
      new molecules with antiplatelet activity and identify their mechanisms of action. 
      Mitoquinone (MitoQ) is a mitochondria-targeted antioxidant that reduces 
      mitochondrial overproduction of ROS. In this work, the antiplatelet effect of 
      MitoQ through platelet adhesion and spreading, secretion, and aggregation was 
      evaluated. Thus MitoQ, in a non-toxic effect, decreased platelet adhesion and 
      spreading on collagen surface, and expression of P-selectin and CD63, and 
      inhibited platelet aggregation induced by collagen, convulxin, thrombin receptor 
      activator peptide-6 (TRAP-6), and phorbol 12-myristate 13-acetate (PMA). As an 
      antiplatelet mechanism, we showed that MitoQ produced mitochondrial 
      depolarization and decreased ATP secretion. Additionally, in platelets stimulated 
      with antimycin A and collagen MitoQ significantly decreased ROS production. Our 
      findings showed, for the first time, an antiplatelet effect of MitoQ that is 
      probably associated with its mitochondrial antioxidant effect.
FAU - Mendez, Diego
AU  - Mendez D
AUID- ORCID: 0000-0003-0156-2913
AD  - Thrombosis Research Center, Medical Technology School, Department of Clinical 
      Biochemistry and Immunohaematology, Faculty of Health Sciences, Universidad de 
      Talca, Talca 3460000, Chile.
FAU - Arauna, Diego
AU  - Arauna D
AUID- ORCID: 0000-0001-7132-991X
AD  - Thrombosis Research Center, Medical Technology School, Department of Clinical 
      Biochemistry and Immunohaematology, Faculty of Health Sciences, Universidad de 
      Talca, Talca 3460000, Chile.
FAU - Fuentes, Francisco
AU  - Fuentes F
AD  - Escuela de Medicina, Universidad de Talca, Talca 3460000, Chile.
FAU - Araya-Maturana, Ramiro
AU  - Araya-Maturana R
AUID- ORCID: 0000-0002-5082-9146
AD  - Instituto de Quimica de Recursos Naturales, Universidad de Talca, Talca 3460000, 
      Chile.
FAU - Palomo, Ivan
AU  - Palomo I
AD  - Thrombosis Research Center, Medical Technology School, Department of Clinical 
      Biochemistry and Immunohaematology, Faculty of Health Sciences, Universidad de 
      Talca, Talca 3460000, Chile.
FAU - Alarcon, Marcelo
AU  - Alarcon M
AUID- ORCID: 0000-0001-7596-5382
AD  - Thrombosis Research Center, Medical Technology School, Department of Clinical 
      Biochemistry and Immunohaematology, Faculty of Health Sciences, Universidad de 
      Talca, Talca 3460000, Chile.
FAU - Sebastian, David
AU  - Sebastian D
AUID- ORCID: 0000-0002-7260-3869
AD  - Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of 
      Science and Technology, 08007 Barcelona, Spain.
AD  - Departament de Bioquimica i Biomedicina Molecular, Facultat de Biologia, 
      Universitat de Barcelona, 08007 Barcelona, Spain.
AD  - Centro de Investigacion Biomedica en Red de Diabetes y Enfermedades Metabolicas 
      Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain.
FAU - Zorzano, Antonio
AU  - Zorzano A
AD  - Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of 
      Science and Technology, 08007 Barcelona, Spain.
AD  - Departament de Bioquimica i Biomedicina Molecular, Facultat de Biologia, 
      Universitat de Barcelona, 08007 Barcelona, Spain.
AD  - Centro de Investigacion Biomedica en Red de Diabetes y Enfermedades Metabolicas 
      Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain.
FAU - Fuentes, Eduardo
AU  - Fuentes E
AUID- ORCID: 0000-0003-0099-4108
AD  - Thrombosis Research Center, Medical Technology School, Department of Clinical 
      Biochemistry and Immunohaematology, Faculty of Health Sciences, Universidad de 
      Talca, Talca 3460000, Chile.
LA  - eng
PT  - Journal Article
DEP - 20200827
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antioxidants)
RN  - 0 (CD63 protein, human)
RN  - 0 (Oligopeptides)
RN  - 0 (Organophosphorus Compounds)
RN  - 0 (P-Selectin)
RN  - 0 (Phorbol Esters)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Ser-Phe-Phe-Leu-Arg-Asn)
RN  - 0 (Tetraspanin 30)
RN  - 1339-63-5 (Ubiquinone)
RN  - 20839-06-9 (phorbol-12-myristate)
RN  - 47BYS17IY0 (mitoquinone)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Blood Platelets/drug effects/*metabolism
MH  - Cells, Cultured
MH  - Collagen/metabolism
MH  - Humans
MH  - Mice
MH  - Mitochondria/metabolism
MH  - Oligopeptides/pharmacology
MH  - Organophosphorus Compounds/*pharmacology
MH  - P-Selectin/metabolism
MH  - Phorbol Esters/pharmacology
MH  - Platelet Activation/drug effects
MH  - Reactive Oxygen Species/*metabolism
MH  - Tetraspanin 30/metabolism
MH  - Ubiquinone/*analogs & derivatives/pharmacology
PMC - PMC7503844
OTO - NOTNLM
OT  - MitoQ
OT  - ROS
OT  - mitochondria
OT  - mitoquinone
OT  - platelets
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/02 06:00
MHDA- 2021/02/23 06:00
PMCR- 2020/09/01
CRDT- 2020/09/02 06:00
PHST- 2020/06/30 00:00 [received]
PHST- 2020/08/18 00:00 [revised]
PHST- 2020/08/19 00:00 [accepted]
PHST- 2020/09/02 06:00 [entrez]
PHST- 2020/09/02 06:00 [pubmed]
PHST- 2021/02/23 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21176192 [pii]
AID - ijms-21-06192 [pii]
AID - 10.3390/ijms21176192 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Aug 27;21(17):6192. doi: 10.3390/ijms21176192.