PMID- 32867602
OWN - NLM
STAT- MEDLINE
DCOM- 20210903
LR  - 20210903
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 29
IP  - 21
DP  - 2020 Nov 1
TI  - Effect of Human Umbilical Cord Mesenchymal Stem Cells Transfected with HGF on 
      TGF-beta1/Smad Signaling Pathway in Carbon Tetrachloride-Induced Liver Fibrosis 
      Rats.
PG  - 1395-1406
LID - 10.1089/scd.2020.0060 [doi]
AB  - The research on human umbilical cord-derived mesenchymal stem cells (hUCMSCs) 
      suggests promising therapeutic strategy for ameliorating liver fibrosis and it 
      can be an effective alternative method of orthotopic liver transplantation. 
      Hepatocyte growth factor (HGF) is the most basic cytokine involved in the 
      inhibition of liver fibrosis and promotion of hepatocyte proliferation and 
      regeneration. The objective of this study was to determine the possible mechanism 
      about how the microencapsulated hUCMSCs made by alginate-poly-lysine-alginate 
      (A-P-A) transfected with HGF could ameliorate liver fibrosis through the 
      TGF-beta1/Smad signaling pathway. The microencapsulated cells were divided into four 
      groups: hUCMSC (microcapsules of hUCMSCs), HGF (microcapsules of HGF+hUCMSCs), 
      LV5-NC (microcapsules of LV5-NC, an rLV-EF1a-EGFP+Puro control lentiviral 
      vector+hUCMSCs), and empty microcapsule (microcapsules without any hUCMSCs), and 
      then transplanted by intraperitoneal injection into carbon tetrachloride 
      (CCl(4))-induced liver fibrosis rats, respectively. The results showed that the 
      fibrosis in the hUCMSC, LV5-NC, and HGF groups was significantly alleviated. 
      Moreover, the messenger RNA (mRNA) and protein levels of collagen I, collagen 
      III, alpha-SMA, TGF-beta1, Smad2, and Smad3 were significantly decreased compared with 
      the empty microcapsule group and these indices in HGF group were more decreased 
      compared with hUCMSC and LV5-NC groups. This study indicated that 
      microencapsulated hUCMSCs transfected with HGF could effectively improve 
      CCl(4)-induced rat liver fibrosis and the possible mechanism was closely related 
      to the inhibition of TGF-beta1/Smad signaling pathway.
FAU - Yin, Fei
AU  - Yin F
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, China.
FAU - Wang, Wen-Ying
AU  - Wang WY
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, China.
FAU - Mao, Li-Cui
AU  - Mao LC
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, China.
FAU - Cai, Qi-Qi
AU  - Cai QQ
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, China.
FAU - Jiang, Wen-Hua
AU  - Jiang WH
AD  - Department of Histology and Embryology, Basic Medical College of Jilin 
      University, Changchun, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200924
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Acta2 protein, rat)
RN  - 0 (Actins)
RN  - 0 (Smad Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
RN  - CL2T97X0V0 (Carbon Tetrachloride)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Carbon Tetrachloride
MH  - Collagen/metabolism
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Infant, Newborn
MH  - Liver Cirrhosis/*chemically induced/*therapy
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Rats, Wistar
MH  - Signal Transduction
MH  - Smad Proteins/*metabolism
MH  - *Transfection
MH  - Transforming Growth Factor beta1/*metabolism
MH  - Umbilical Cord/*cytology
OTO - NOTNLM
OT  - A-P-A microcapsule
OT  - TGF-beta1/Smad signaling pathway
OT  - cell therapy
OT  - hepatocyte growth factor
OT  - human umbilical cord mesenchymal stem cells
OT  - liver fibrosis
EDAT- 2020/09/02 06:00
MHDA- 2021/09/04 06:00
CRDT- 2020/09/02 06:00
PHST- 2020/09/02 06:00 [pubmed]
PHST- 2021/09/04 06:00 [medline]
PHST- 2020/09/02 06:00 [entrez]
AID - 10.1089/scd.2020.0060 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2020 Nov 1;29(21):1395-1406. doi: 10.1089/scd.2020.0060. Epub 
      2020 Sep 24.