PMID- 32870261
OWN - NLM
STAT- MEDLINE
DCOM- 20211018
LR  - 20211018
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 103
IP  - 5
DP  - 2020 Oct 29
TI  - Severity of prepregnancy diabetes on the fetal malformations and viability 
      associated with early embryos in ratsdagger.
PG  - 938-950
LID - 10.1093/biolre/ioaa151 [doi]
AB  - Preexisting/pregestational diabetes enhances the risk of birth defects. Several 
      factors have been involved during the implantation process, such as cytokines 
      (granulocyte-macrophage-colony-stimulating factor [GM-CSF]). The objective was to 
      evaluate the effects of two levels of diabetes on the redox status of 
      preimplantation embryos during the implantation process to comprehend how both 
      are involved in embryo and fetal viability against maternal diabetes. Female 
      Sprague-Dawley rats received streptozotocin at birth (mild diabetes [MD]) or at 
      adulthood (severe diabetes [SD]) to obtain two experimental diabetes intensities. 
      After confirming the diabetic status, the nondiabetic and diabetic groups were 
      mated around day 110 of life. At gestational day (GD) 21, fetuses were assessed 
      for viability and malformations and ovaries for embryo loss before implantation. 
      Other pregnant nondiabetic and diabetic rats were sacrificed at GD2-4 for 
      maternal and preimplantation embryo oxidative stress markers, maternal serum 
      insulin, uterine fluid GM-CSF, and preimplantation embryo morphological analysis. 
      MD and SD caused abnormal redox levels, lower GM-CSF and insulin levels during 
      the preimplantation period, and embryonic loss before implantation. SD caused 
      lower fetal viability and higher fetal malformation percentages at GD21. The SD 
      dam-derived preimplantation embryos presented lower glutathione levels and higher 
      thiobarbituric acid reactive substances concentration at GD3 and an increased 
      frequency of abnormal preimplantation embryos at GD4. In conclusion, preexisting 
      diabetes leads to complications in the implantation process. Furthermore, 
      maternal oxidative stress and other metabolic changes alter the redox state and 
      morphological structure of preimplantation embryos, contributing to damaged 
      growth and development in late pregnancy.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction. All rights reserved. For permissions, please 
      e-mail: journals.permissions@oup.com.
FAU - Bueno, Aline
AU  - Bueno A
AD  - Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate 
      Course on Tocogynecology, Botucatu Medical School, Sao Paulo State University 
      (Unesp), Botucatu, Sao Paulo State, Brazil.
FAU - Sinzato, Yuri Karen
AU  - Sinzato YK
AD  - Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate 
      Course on Tocogynecology, Botucatu Medical School, Sao Paulo State University 
      (Unesp), Botucatu, Sao Paulo State, Brazil.
FAU - Volpato, Gustavo Tadeu
AU  - Volpato GT
AD  - Laboratory of System Physiology and Reproductive Toxicology, Institute of 
      Biological and Health Sciences, Federal University of Mato Grosso (UFMT), Barra 
      do Garcas, Mato Grosso State, Brazil.
FAU - Gallego, Franciane Quintanilha
AU  - Gallego FQ
AD  - Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate 
      Course on Tocogynecology, Botucatu Medical School, Sao Paulo State University 
      (Unesp), Botucatu, Sao Paulo State, Brazil.
FAU - Perecin, Felipe
AU  - Perecin F
AD  - Faculty of Animal Science and Food Engineering, Department of Veterinary 
      Medicine, University of Sao Paulo (USP), Pirassununga, Sao Paulo State, Brazil.
FAU - Rodrigues, Tiago
AU  - Rodrigues T
AD  - Center of Natural and Human Sciences (CCNH), Federal University of ABC (UFABC), 
      Santo Andre, Sao Paulo State, Brazil.
FAU - Damasceno, Debora Cristina
AU  - Damasceno DC
AD  - Laboratory of Experimental Research on Gynecology and Obstetrics, Postgraduate 
      Course on Tocogynecology, Botucatu Medical School, Sao Paulo State University 
      (Unesp), Botucatu, Sao Paulo State, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
SB  - IM
MH  - Animals
MH  - Congenital Abnormalities/*etiology/metabolism
MH  - Diabetes Mellitus, Experimental/*complications/metabolism
MH  - Embryo Implantation/physiology
MH  - Embryonic Development/*physiology
MH  - Female
MH  - Oxidative Stress/physiology
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - fetus
OT  - hyperglycemia
OT  - oxidative stress
OT  - pregestational
OT  - rat
EDAT- 2020/09/02 06:00
MHDA- 2021/10/21 06:00
CRDT- 2020/09/02 06:00
PHST- 2020/05/05 00:00 [received]
PHST- 2020/08/12 00:00 [revised]
PHST- 2020/08/22 00:00 [accepted]
PHST- 2020/09/02 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2020/09/02 06:00 [entrez]
AID - 5900057 [pii]
AID - 10.1093/biolre/ioaa151 [doi]
PST - ppublish
SO  - Biol Reprod. 2020 Oct 29;103(5):938-950. doi: 10.1093/biolre/ioaa151.