PMID- 32871756
OWN - NLM
STAT- MEDLINE
DCOM- 20210618
LR  - 20210618
IS  - 1791-7549 (Electronic)
IS  - 0258-851X (Print)
IS  - 0258-851X (Linking)
VI  - 34
IP  - 5
DP  - 2020 Sep-Oct
TI  - Deep Sequencing Identified Dysregulated Circulating MicroRNAs in Late Onset 
      Preeclampsia.
PG  - 2317-2324
LID - 10.21873/invivo.12044 [doi]
AB  - BACKGROUND/AIM: To characterize global microRNA (miRNA) expression profile in the 
      first trimester maternal plasma of women who subsequently develop late-onset 
      preeclampsia (LOPE) compared to uncomplicated pregnancies. MATERIALS AND METHODS: 
      Five first trimester plasma samples from women who developed LOPE and 5 controls 
      were analyzed using next generation sequencing technology (NGS) followed by 
      target prediction, Gene Ontology analysis and pathway identification. 
      Quantitative real-time polymerase chain reaction (qRT-PCR) was performed for 
      confirmation in an independent cohort of 12 LOPE cases and 12 controls. RESULTS: 
      miR-23b-5p and miR-99b-5p were down-regulated by >1.5 fold in LOPE complicated 
      pregnancies (p value <0.05) compared to controls. Target prediction showed that 
      the major targets of these miRNAs are associated with glycometabolism and immune 
      response. CONCLUSION: miR-23b-5p and miR-99b-5p are possibly implicated in the 
      pathogenic mechanisms leading to the induction of LOPE and may serve as candidate 
      non-invasive biomarkers for early prediction and prevention.
CI  - Copyright(c) 2020, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Mavreli, Danai
AU  - Mavreli D
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
AD  - Department of Medical Genetics, National and Kapodistrian University of Athens, 
      Athens, Greece.
FAU - Lykoudi, Alexandra
AU  - Lykoudi A
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
AD  - Department of Medical Genetics, National and Kapodistrian University of Athens, 
      Athens, Greece.
FAU - Lambrou, George
AU  - Lambrou G
AD  - 1 Department of Pediatrics, National and Kapodistrian University of Athens, 
      Athens, Greece.
FAU - Papaioannou, George
AU  - Papaioannou G
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Vrachnis, Nikolas
AU  - Vrachnis N
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Kalantaridou, Sophia
AU  - Kalantaridou S
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Papantoniou, Nikolas
AU  - Papantoniou N
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece.
FAU - Kolialexi, Aggeliki
AU  - Kolialexi A
AD  - 3 Department of Obstetrics and Gynecology, National and Kapodistrian University 
      of Athens, Athens, Greece akolial@med.uoa.gr.
AD  - Department of Medical Genetics, National and Kapodistrian University of Athens, 
      Athens, Greece.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - In Vivo
JT  - In vivo (Athens, Greece)
JID - 8806809
RN  - 0 (Biomarkers)
RN  - 0 (Circulating MicroRNA)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Biomarkers
MH  - *Circulating MicroRNA/genetics
MH  - Female
MH  - Gene Expression Profiling
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - *MicroRNAs/genetics
MH  - *Pre-Eclampsia/diagnosis/genetics
MH  - Pregnancy
PMC - PMC7652460
OTO - NOTNLM
OT  - NGS
OT  - late-onset preeclampsia
OT  - miR-23b-5p
OT  - miR-99b-5p
OT  - microRNAs
OT  - preeclampsia
COIS- The Authors have no conflicts of interest to declare in relation to this study.
EDAT- 2020/09/03 06:00
MHDA- 2021/06/22 06:00
PMCR- 2020/09/03
CRDT- 2020/09/03 06:00
PHST- 2020/05/10 00:00 [received]
PHST- 2020/05/28 00:00 [revised]
PHST- 2020/05/29 00:00 [accepted]
PHST- 2020/09/03 06:00 [entrez]
PHST- 2020/09/03 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/09/03 00:00 [pmc-release]
AID - 34/5/2317 [pii]
AID - 10.21873/invivo.12044 [doi]
PST - ppublish
SO  - In Vivo. 2020 Sep-Oct;34(5):2317-2324. doi: 10.21873/invivo.12044.