PMID- 32872428
OWN - NLM
STAT- MEDLINE
DCOM- 20210223
LR  - 20210223
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Aug 29
TI  - Exhaustive Search of the Receptor Ligands by the CyCLOPS (Cytometry Cell-Labeling 
      Operable Phage Screening) Technique.
LID - 10.3390/ijms21176258 [doi]
LID - 6258
AB  - Effective and versatile screening of the peptide ligands capable of selectively 
      binding to diverse receptors is in high demand for the state-of-the-art 
      technologies in life sciences, including probing of specificity of the cell 
      surface receptors and drug development. Complex microenvironment and structure of 
      the surface receptors significantly reduce the possibility to determine their 
      specificity, especially when in vitro conditions are utilized. Previously, we 
      designed a publicly available platform for the ultra-high-throughput screening 
      (uHTS) of the specificity of surface-exposed receptors of the living eukaryotic 
      cells, which was done by consolidating the phage display and flow cytometry 
      techniques. Here, we significantly improved this methodology and designed the 
      fADL-1e-based phage vectors that do not require a helper hyperphage for the 
      virion assembly. The enhanced screening procedure was tested on soluble human 
      leukocyte antigen (HLA) class II molecules and transgenic antigen-specific B 
      cells that express recombinant lymphoid B-cell receptor (BCR). Our data suggest 
      that the improved vector system may be successfully used for the comprehensive 
      search of the receptor ligands in either cell-based or surface-immobilized 
      assays.
FAU - Ishina, Irina A
AU  - Ishina IA
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
FAU - Filimonova, Ioanna N
AU  - Filimonova IN
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
FAU - Zakharova, Maria Y
AU  - Zakharova MY
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
AD  - Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
FAU - Ovchinnikova, Leyla A
AU  - Ovchinnikova LA
AUID- ORCID: 0000-0002-3086-0728
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
FAU - Mamedov, Azad E
AU  - Mamedov AE
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
FAU - Lomakin, Yakov A
AU  - Lomakin YA
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
FAU - Belogurov, Alexey A Jr
AU  - Belogurov AA Jr
AUID- ORCID: 0000-0002-2033-9621
AD  - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 117997 Moscow, 
      Russia.
AD  - Lomonosov Moscow State University, 119991 Moscow, Russia.
LA  - eng
GR  - 17-74-30019/Russian Science Foundation/
PT  - Journal Article
DEP - 20200829
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Antigen, B-Cell)
SB  - IM
MH  - Bacteriophage M13/genetics
MH  - Cell Line
MH  - Cell Surface Display Techniques/*methods
MH  - Histocompatibility Antigens Class II/*analysis/genetics
MH  - Humans
MH  - Ligands
MH  - Receptors, Antigen, B-Cell/*analysis/genetics
PMC - PMC7504098
OTO - NOTNLM
OT  - BCR
OT  - FACS
OT  - HLA-DRB1
OT  - M13 bacteriophage
OT  - MHC II
OT  - antigen-specific B cell
OT  - fd bacteriophage
OT  - filamentous phage
OT  - immunoglobulin selection
OT  - ligand-receptor interaction
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/03 06:00
MHDA- 2021/02/24 06:00
PMCR- 2020/09/01
CRDT- 2020/09/03 06:00
PHST- 2020/06/09 00:00 [received]
PHST- 2020/08/25 00:00 [revised]
PHST- 2020/08/27 00:00 [accepted]
PHST- 2020/09/03 06:00 [entrez]
PHST- 2020/09/03 06:00 [pubmed]
PHST- 2021/02/24 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21176258 [pii]
AID - ijms-21-06258 [pii]
AID - 10.3390/ijms21176258 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Aug 29;21(17):6258. doi: 10.3390/ijms21176258.