PMID- 32875631
OWN - NLM
STAT- MEDLINE
DCOM- 20210708
LR  - 20240215
IS  - 1521-4095 (Electronic)
IS  - 0935-9648 (Print)
IS  - 0935-9648 (Linking)
VI  - 32
IP  - 40
DP  - 2020 Oct
TI  - Reduced Graphene Oxide-Encapsulated Microfiber Patterns Enable Controllable 
      Formation of Neuronal-Like Networks.
PG  - e2004555
LID - 10.1002/adma.202004555 [doi]
AB  - Scaffold-guided formation of neuronal-like networks, especially under electrical 
      stimulation, can be an appealing avenue toward functional restoration of injured 
      nervous systems. Here, 3D conductive scaffolds are fabricated based on printed 
      microfiber constructs using near-field electrostatic printing (NFEP) and graphene 
      oxide (GO) coating. Various microfiber patterns are obtained from poly(l-lactic 
      acid-co-caprolactone) (PLCL) using NFEP and complexity is achieved via modulating 
      the fiber overlay angles (45 degrees , 60 degrees , 75 degrees , 90 degrees ), fiber diameters (15 to 148 microm), 
      and fiber spatial organization (spider web and tubular structure). Upon coating 
      GO onto PLCL microfibers via a layer-by-layer (L-b-L) assembly technique and in 
      situ reduction into reduced GO (rGO), the obtained conductive scaffolds, with 
      25-50 layers of rGO, demonstrate superior conductivity ( approximately 0.95 S cm(-1) ) and 
      capability of inducing neuronal-like network formation along the conductive 
      microfibers under electrical stimulation (100-150 mV cm(-1) ). Both electric 
      field (0-150 mV cm(-1) ) and microfiber diameter (17-150 microm) affect neurite 
      outgrowth (PC-12 cells and primary mouse hippocampal neurons) and the formation 
      of orientated neuronal-like networks. With further demonstration of such guidance 
      to neuronal cells, these conductive scaffolds may see versatile applications in 
      nerve regeneration and neural engineering.
CI  - (c) 2020 Wiley-VCH GmbH.
FAU - Wang, Juan
AU  - Wang J
AD  - Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, 
      NJ, 07030, USA.
AD  - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, 
      College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai, 201620, P. R. China.
FAU - Wang, Haoyu
AU  - Wang H
AD  - Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, 
      NJ, 07030, USA.
AD  - Department of Chemistry and Chemical Biology, Stevens Institute of Technology, 
      Hoboken, NJ, 07030, USA.
FAU - Mo, Xiumei
AU  - Mo X
AD  - State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, 
      College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, 
      Shanghai, 201620, P. R. China.
FAU - Wang, Hongjun
AU  - Wang H
AUID- ORCID: 0000-0003-3455-8523
AD  - Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, 
      NJ, 07030, USA.
AD  - Department of Chemistry and Chemical Biology, Stevens Institute of Technology, 
      Hoboken, NJ, 07030, USA.
LA  - eng
GR  - R01 AR067859/AR/NIAMS NIH HHS/United States
GR  - 1R01AR067859/AR/NIAMS NIH HHS/United States
PT  - Journal Article
DEP - 20200902
PL  - Germany
TA  - Adv Mater
JT  - Advanced materials (Deerfield Beach, Fla.)
JID - 9885358
RN  - 0 (Biocompatible Materials)
RN  - 0 (Capsules)
RN  - 0 (graphene oxide)
RN  - 7782-42-5 (Graphite)
SB  - IM
MH  - Animals
MH  - Biocompatible Materials/*chemistry/*pharmacology
MH  - Capsules
MH  - Cell Engineering
MH  - Graphite/*chemistry
MH  - Mice
MH  - Microtechnology/*methods
MH  - Nerve Regeneration/drug effects
MH  - Neurons/*cytology/drug effects
MH  - Printing
MH  - Static Electricity
MH  - Tissue Scaffolds/*chemistry
PMC - PMC10865229
MID - NIHMS1944353
OTO - NOTNLM
OT  - conductive micropatterns
OT  - electrical stimulation
OT  - graphene oxide
OT  - microfibers
OT  - neuronal-like networks
COIS- Conflict of Interest The authors declare no conflict of interest.
EDAT- 2020/09/03 06:00
MHDA- 2021/07/09 06:00
PMCR- 2024/02/14
CRDT- 2020/09/03 06:00
PHST- 2020/07/04 00:00 [received]
PHST- 2020/07/31 00:00 [revised]
PHST- 2020/09/03 06:00 [pubmed]
PHST- 2021/07/09 06:00 [medline]
PHST- 2020/09/03 06:00 [entrez]
PHST- 2024/02/14 00:00 [pmc-release]
AID - 10.1002/adma.202004555 [doi]
PST - ppublish
SO  - Adv Mater. 2020 Oct;32(40):e2004555. doi: 10.1002/adma.202004555. Epub 2020 Sep 
      2.