PMID- 32877612
OWN - NLM
STAT- MEDLINE
DCOM- 20210823
LR  - 20210823
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 63
IP  - 12
DP  - 2020 Dec
TI  - Development of alien addition lines from Cucumis hystrix in Cucumis sativus: 
      cytological and molecular marker analyses.
PG  - 629-641
LID - 10.1139/gen-2020-0035 [doi]
AB  - Transferring desired genes from wild species to cultivars through alien addition 
      lines (AALs) has been shown to be an effective method for genetic improvement. 
      Cucumis hystrix Chakr. (HH, 2n = 24) is a wild species of Cucumis that possesses 
      many resistant genes. A synthetic allotetraploid species, C. hytivus (HHCC, 2n = 
      38), was obtained from the cross between cultivated cucumber, C. sativus (CC, 2n 
      = 14), and C. hystrix followed by chromosome doubling. Cucumis sativus - C. 
      hystrix AALs were developed by continuous backcrossing to the cultivated 
      cucumbers. In this study, 10 different types of AALs (CC-H01, CC-H06, CC-H08, 
      CC-H10, CC-H12, CC-H06+H09, CC-H06+H10, CC-H06+H12, CC-H08+H10, CC-H01+H06+H10) 
      were identified based on the analysis of fluorescence in situ hybridization 
      (FISH) and molecular markers specific to C. hystrix chromosomes. And the behavior 
      of the alien chromosomes in three AALs (CC-H01, CC-H06+H10, CC-H01+H06+H10) at 
      meiosis was investigated. The results showed that alien chromosomes paired with 
      C. sativus chromosome in few pollen mother cells (PMCs). Further, disomic alien 
      addition lines (DAALs) carrying a pair of C. hystrix chromosome H10 were screened 
      from the selfed progenies of CC-H10. Chromosome pairing between genomes provides 
      cytological evidence for the possible introgression of alien chromosome segments. 
      The development of AALs could serve as a key step for exploiting and utilizing 
      valuable genes from C. hystrix.
FAU - Li, Mengxue
AU  - Li M
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Zhao, Qinzheng
AU  - Zhao Q
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Liu, Yuxi
AU  - Liu Y
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Qin, Xiaodong
AU  - Qin X
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Hu, Wei
AU  - Hu W
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Davoudi, Marzieh
AU  - Davoudi M
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Chen, Jinfeng
AU  - Chen J
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
FAU - Lou, Qunfeng
AU  - Lou Q
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, College of 
      Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
LA  - eng
PT  - Journal Article
DEP - 20200902
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Chromosomes, Plant
MH  - Cucumis/*genetics
MH  - Cucumis sativus/*genetics
MH  - *Genome, Plant
MH  - Hybridization, Genetic
MH  - In Situ Hybridization, Fluorescence
MH  - Meiosis
MH  - Phenotype
MH  - Species Specificity
OTO - NOTNLM
OT  - Cucumis hystrix Chakr.
OT  - FISH
OT  - alien addition lines
OT  - appariement des chromosomes
OT  - chromosome pairing
OT  - lignees d'addition
OT  - marqueurs moleculaires
OT  - molecular markers
EDAT- 2020/09/03 06:00
MHDA- 2021/08/24 06:00
CRDT- 2020/09/03 06:00
PHST- 2020/09/03 06:00 [pubmed]
PHST- 2021/08/24 06:00 [medline]
PHST- 2020/09/03 06:00 [entrez]
AID - 10.1139/gen-2020-0035 [doi]
PST - ppublish
SO  - Genome. 2020 Dec;63(12):629-641. doi: 10.1139/gen-2020-0035. Epub 2020 Sep 2.