PMID- 32877992
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201027
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 8
IP  - 3
DP  - 2020 Aug 31
TI  - Interferon Inducing Porcine Reproductive and Respiratory Syndrome Virus Vaccine 
      Candidate Protected Piglets from HP-PRRSV Challenge and Evoke a Higher Level of 
      Neutralizing Antibodies Response.
LID - 10.3390/vaccines8030490 [doi]
LID - 490
AB  - Although widespread administration of attenuated porcine reproductive and 
      respiratory syndrome virus (PRRSV) vaccines has been implemented since they first 
      became commercially available two decades ago, PRRSV infection prevalence in 
      swine herds remains high. The limited success of PRRSV vaccines is partly due to 
      the well-established fact that a given vaccine strain confers only partial or no 
      protection against heterologous strains. In our past work, A2MC2-P90, a novel 
      PRRSV vaccine candidate that induced a type I IFNs response in vitro, conferred 
      complete protection against challenge with genetically heterologous PRRSV 
      strains. Here we assessed the ability of the PRRSV vaccine candidate A2MC2-P90 to 
      protect piglets against the HP-PRRSV challenge and compared its efficacy to that 
      of a licensed HP-PRRSV-specific vaccine (TJM-F92) assessed in parallel. A2MC2-P90 
      provided vaccinated piglets with 100% protection from a lethal challenge with 
      extremely virulent HP-PRRSV-XJA1, while 100% mortality was observed for 
      unvaccinated piglets by day 21 post-challenge. Notably, comparison of partial 
      sequence (GP5) of XJA1 to A2MC2-P90 suggested there was only 88.7% homology. When 
      comparing post-HP-PRRSV challenge responses between piglets administered 
      A2AMC2-P90 versus those immunized with licensed vaccine TJM-F92, 
      A2MC2-P90-vaccinated piglets rapidly developed a stronger protective humoral 
      immune response, as evidenced by much higher titers of neutralizing antibodies, 
      more rapid clearance of viremia and less nasal virus shedding. In conclusion, our 
      data suggest that this novel vaccine candidate A2MC2-P90 has improved protection 
      spectrum against heterologous HP-PRRSV strains.
FAU - Li, Yafei
AU  - Li Y
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China.
AD  - Scientific Observing and Experimental Station of Veterinary Pharmacology and 
      Veterinary Biotechnology, Ministry of Agriculture, Yangling 712100, China.
FAU - Li, Junhui
AU  - Li J
AD  - Tecon Biology Co., Ltd., Urumqi 830000, Xinjiang, China.
FAU - He, Sun
AU  - He S
AD  - Tecon Biology Co., Ltd., Urumqi 830000, Xinjiang, China.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Tecon Biology Co., Ltd., Urumqi 830000, Xinjiang, China.
FAU - Cao, Jian
AU  - Cao J
AD  - Tecon Biology Co., Ltd., Urumqi 830000, Xinjiang, China.
FAU - Pan, Xiaomei
AU  - Pan X
AD  - Tecon Biology Co., Ltd., Urumqi 830000, Xinjiang, China.
FAU - Tang, Huifen
AU  - Tang H
AD  - Tecon Biology Co., Ltd., Urumqi 830000, Xinjiang, China.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China.
AD  - Scientific Observing and Experimental Station of Veterinary Pharmacology and 
      Veterinary Biotechnology, Ministry of Agriculture, Yangling 712100, China.
FAU - Wu, Chunyan
AU  - Wu C
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China.
AD  - Scientific Observing and Experimental Station of Veterinary Pharmacology and 
      Veterinary Biotechnology, Ministry of Agriculture, Yangling 712100, China.
FAU - Nan, Yuchen
AU  - Nan Y
AUID- ORCID: 0000-0002-4442-8004
AD  - Department of Preventive Veterinary Medicine, College of Veterinary Medicine, 
      Northwest A&F University, Yangling 712100, China.
AD  - Scientific Observing and Experimental Station of Veterinary Pharmacology and 
      Veterinary Biotechnology, Ministry of Agriculture, Yangling 712100, China.
LA  - eng
GR  - 31672534/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20200831
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC7565719
OTO - NOTNLM
OT  - HP-PRRSV
OT  - IFN induction
OT  - PRRSV
OT  - modified live vaccines
OT  - neutralizing antibodies
OT  - protection
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/04 06:00
MHDA- 2020/09/04 06:01
PMCR- 2020/08/31
CRDT- 2020/09/04 06:00
PHST- 2020/08/03 00:00 [received]
PHST- 2020/08/17 00:00 [revised]
PHST- 2020/08/21 00:00 [accepted]
PHST- 2020/09/04 06:00 [entrez]
PHST- 2020/09/04 06:00 [pubmed]
PHST- 2020/09/04 06:01 [medline]
PHST- 2020/08/31 00:00 [pmc-release]
AID - vaccines8030490 [pii]
AID - vaccines-08-00490 [pii]
AID - 10.3390/vaccines8030490 [doi]
PST - epublish
SO  - Vaccines (Basel). 2020 Aug 31;8(3):490. doi: 10.3390/vaccines8030490.