PMID- 32878262
OWN - NLM
STAT- MEDLINE
DCOM- 20210907
LR  - 20210907
IS  - 2218-273X (Electronic)
IS  - 2218-273X (Linking)
VI  - 10
IP  - 9
DP  - 2020 Aug 31
TI  - Branched-Chain Fatty Acids as Mediators of the Activation of Hepatic Peroxisome 
      Proliferator-Activated Receptor Alpha by a Fungal Lipid Extract.
LID - 10.3390/biom10091259 [doi]
LID - 1259
AB  - The study aimed to test the hypothesis that monomethyl branched-chain fatty acids 
      (BCFAs) and a lipid extract of Conidiobolus heterosporus (CHLE), rich in 
      monomethyl BCFAs, are able to activate the nuclear transcription factor 
      peroxisome proliferator-activated receptor alpha (PPARalpha). Rat Fao cells were 
      incubated with the monomethyl BCFAs 12-methyltridecanoic acid (MTriA), 
      12-methyltetradecanoic acid (MTA), isopalmitic acid (IPA) and 
      14-methylhexadecanoic acid (MHD), and the direct activation of PPARalpha was 
      evaluated by reporter gene assay using a PPARalpha responsive reporter gene. 
      Furthermore, Fao cells were incubated with different concentrations of the CHLE 
      and PPARalpha activation was also evaluated by using the reporter gene assay, and 
      by determining the mRNA concentrations of selected PPARalpha target genes by 
      real-time RT-PCR. The reporter gene assay revealed that IPA and the CHLE, but not 
      MTriA, MHD and MTA, activate the PPARalpha responsive reporter gene. CHLE 
      dose-dependently increased mRNA concentrations of the PPARalpha target genes 
      acyl-CoA oxidase (ACOX1), cytochrome P450 4A1 (CYP4A1), carnitine 
      palmitoyltransferase 1A (CPT1A) and solute carrier family 22 (organic 
      cation/carnitine transporter), member 5 (SLC22A5). In conclusion, the monomethyl 
      BCFA IPA is a potent PPARalpha activator. CHLE activates PPARalpha-dependent gene 
      expression in Fao cells, an effect that is possibly mediated by IPA.
FAU - Maheshwari, Garima
AU  - Maheshwari G
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
AD  - Institute of Food Chemistry and Food Biotechnology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany.
FAU - Ringseis, Robert
AU  - Ringseis R
AUID- ORCID: 0000-0002-1321-4820
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
FAU - Wen, Gaiping
AU  - Wen G
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
FAU - Gessner, Denise K
AU  - Gessner DK
AUID- ORCID: 0000-0002-7934-1304
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
FAU - Rost, Johanna
AU  - Rost J
AD  - Institute of Food Chemistry and Food Biotechnology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany.
FAU - Fraatz, Marco A
AU  - Fraatz MA
AUID- ORCID: 0000-0002-5028-9653
AD  - Institute of Food Chemistry and Food Biotechnology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany.
FAU - Zorn, Holger
AU  - Zorn H
AUID- ORCID: 0000-0002-8383-8196
AD  - Institute of Food Chemistry and Food Biotechnology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, Germany.
AD  - Fraunhofer Institute for Molecular Biology and Applied Ecology, Winchester Str. 
      2, 35394 Giessen, Germany.
FAU - Eder, Klaus
AU  - Eder K
AD  - Institute of Animal Nutrition and Nutrition Physiology, Justus Liebig University 
      Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.
LA  - eng
GR  - Insect Biotechnology and Bioresoursces/Hessisches Ministerium fur Wissenschaft 
      und Kunst/International
GR  - ID 57243780/Deutscher Akademischer Austauschdienst/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200831
PL  - Switzerland
TA  - Biomolecules
JT  - Biomolecules
JID - 101596414
RN  - 0 (Fatty Acids)
RN  - 0 (PPAR alpha)
RN  - 0 (Transcription Factors)
RN  - Conidiobolus heterosporus
SB  - IM
MH  - Animals
MH  - Cell Line, Tumor
MH  - Conidiobolus/*chemistry
MH  - Fatty Acids/*metabolism
MH  - Gene Expression Regulation
MH  - Genes, Reporter
MH  - PPAR alpha/agonists/*metabolism
MH  - Rats
MH  - Transcription Factors/*metabolism
PMC - PMC7565516
OTO - NOTNLM
OT  - Conidiobolus heterosporus
OT  - branched-chain fatty acids
OT  - fatty acid oxidation
OT  - hepatocyte.
OT  - lipid metabolism
OT  - peroxisome proliferator-activated receptor alpha
COIS- The authors declare no conflict of interest.
EDAT- 2020/09/04 06:00
MHDA- 2021/09/08 06:00
PMCR- 2020/09/01
CRDT- 2020/09/04 06:00
PHST- 2020/06/08 00:00 [received]
PHST- 2020/08/26 00:00 [revised]
PHST- 2020/08/28 00:00 [accepted]
PHST- 2020/09/04 06:00 [entrez]
PHST- 2020/09/04 06:00 [pubmed]
PHST- 2021/09/08 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - biom10091259 [pii]
AID - biomolecules-10-01259 [pii]
AID - 10.3390/biom10091259 [doi]
PST - epublish
SO  - Biomolecules. 2020 Aug 31;10(9):1259. doi: 10.3390/biom10091259.