PMID- 32880379
OWN - NLM
STAT- MEDLINE
DCOM- 20201015
LR  - 20201015
IS  - 0177-5103 (Print)
IS  - 0177-5103 (Linking)
VI  - 140
DP  - 2020 Sep 3
TI  - First detection of infectious haematopoietic necrosis virus in farmed rainbow 
      trout in North Macedonia.
PG  - 219-225
LID - 10.3354/dao03507 [doi]
AB  - Infectious haematopoietic necrosis virus (IHNV) is the causative agent of 
      infectious haematopoietic necrosis (IHN), a disease of salmonids responsible for 
      great economic losses. The disease occurs in most parts of the world where 
      rainbow trout is reared but has not been previously reported in North Macedonia. 
      In this study, 150 pooled samples in total, each consisting of organ mix of 10 
      freshly killed rainbow trout Oncorhynchus mykiss, were collected from 50 trout 
      farms by the Food and Veterinary Agency of North Macedonia as part of the annual 
      surveillance plan for IHN and viral haemorrhagic septicaemia (VHS) control. 
      Screening of samples was done by cell culture and real-time RT-PCR (qRT-PCR). All 
      150 tested samples were VHS virus (VHSV) qRT-PCR negative. Two samples from 
      different trout farms were IHNV qRT-PCR positive. On cell culture, 1 IHNV qRT-PCR 
      positive sample caused cytopathic effect after 2 passages on EPC cells. The 
      virus, isolated from an asymptomatic rainbow trout fry, was identified by qRT-PCR 
      and designated as MAKIHNV1. The phylogenetic reconstruction indicates that the 
      isolated virus belongs to the European E genogroup, more specifically within the 
      E-1 clade, and is similar to the German, Italian and Iranian isolates. This study 
      has revealed for the first time the presence of IHNV in rainbow trout in North 
      Macedonia. However, it is not possible to make interpretations about the source 
      of infection from the phylogenetic analysis, and the origin of MAKIHNV1 remains 
      unclear.
FAU - Cvetkovikj, Aleksandar
AU  - Cvetkovikj A
AD  - Veterinary Institute, Faculty of Veterinary Medicine-Skopje, Ss. Cyril and 
      Methodius University in Skopje, Lazar Pop-Trajkov 5-7, 1000 Skopje, North 
      Macedonia.
FAU - Radosavljevic, Vladimir
AU  - Radosavljevic V
FAU - Cuenca, Argelia
AU  - Cuenca A
FAU - Strojmanovska, Biljana
AU  - Strojmanovska B
FAU - Maksimovic-Zoric, Jelena
AU  - Maksimovic-Zoric J
FAU - Cvetkovikj, Iskra
AU  - Cvetkovikj I
FAU - Olesen, Niels Jorgen
AU  - Olesen NJ
LA  - eng
PT  - Journal Article
DEP - 20200903
PL  - Germany
TA  - Dis Aquat Organ
JT  - Diseases of aquatic organisms
JID - 8807037
SB  - IM
MH  - Animals
MH  - *Fish Diseases
MH  - *Infectious hematopoietic necrosis virus
MH  - *Oncorhynchus mykiss
MH  - Phylogeny
MH  - Rhabdoviridae Infections/*veterinary
OTO - NOTNLM
OT  - IHNV
OT  - Infectious haematopoietic necrosis virus
OT  - Phylogeny
OT  - Rainbow trout
EDAT- 2020/09/04 06:00
MHDA- 2020/10/21 06:00
CRDT- 2020/09/04 06:00
PHST- 2020/09/04 06:00 [entrez]
PHST- 2020/09/04 06:00 [pubmed]
PHST- 2020/10/21 06:00 [medline]
AID - 10.3354/dao03507 [doi]
PST - epublish
SO  - Dis Aquat Organ. 2020 Sep 3;140:219-225. doi: 10.3354/dao03507.