PMID- 32881004
OWN - NLM
STAT- MEDLINE
DCOM- 20210212
LR  - 20210212
IS  - 1365-3024 (Electronic)
IS  - 0141-9838 (Linking)
VI  - 42
IP  - 12
DP  - 2020 Dec
TI  - Trichomonas vaginalis-secreted cysteinyl leukotrienes promote migration, 
      degranulation and MCP-1 production in mast cells.
PG  - e12789
LID - 10.1111/pim.12789 [doi]
AB  - Trichomonas vaginalis, a flagellated extracellular protozoan parasite that 
      infects the human genitourinary tract, is usually transmitted by sexual contact. 
      Our previous study showed that the leukotriene B(4) (LTB(4) ), a 
      T vaginalis-secreted lipid mediator, induces interleukin (IL)-8 production and 
      promotes mast cell degranulation and migration via BLT1 in human. In this study, 
      we investigated whether T vaginalis produces another leukotrienes and whether it 
      causes increased MCP-1 production, mast cell migration and degranulation by 
      activating mast cells. We found that cysteinyl leukotrienes (CysLTs) were 
      contained in T vaginalis-derived secretory product (TvSP) by ELISA. The 
      TvSP-stimulated human mast cell line (HMC-1) exhibited significantly increased 
      monocyte chemoattractant protein-1 (MCP-1) secretion compared to the unstimulated 
      cells. Inhibition of NOX2 activation of cells by treatment of NOX inhibitor or 
      NOX2 siRNA reduced TvSP-stimulated MCP-1 production in HMC-1 cells. It was also 
      confirmed that the receptor for CysLTs is expressed in mast cells. The CysLT 
      receptor (CysLTR) antagonist inhibited TvSP-stimulated MCP-1 production of mast 
      cells, as well as ROS production, migration and degranulation of mast cells, and 
      reduced phospho-NF-kB expression. These results suggest that T vaginalis-secreted 
      CysLTs promote migration, degranulation and MCP-1 production in human mast cells 
      through CysLT receptor-mediated NOX2 activation.
CI  - (c) 2020 John Wiley & Sons Ltd.
FAU - Lee, Young Ah
AU  - Lee YA
AUID- ORCID: 0000-0002-0414-842X
AD  - Department of Environmental Medical Biology and Institute of Tropical Medicine, 
      Yonsei University College of Medicine, Seoul, Korea.
FAU - Nam, Young Hee
AU  - Nam YH
AD  - Department of Environmental Medical Biology and Institute of Tropical Medicine, 
      Yonsei University College of Medicine, Seoul, Korea.
FAU - Min, Arim
AU  - Min A
AUID- ORCID: 0000-0003-2938-9630
AD  - Department of Environmental Medical Biology and Institute of Tropical Medicine, 
      Yonsei University College of Medicine, Seoul, Korea.
FAU - Shin, Myeong Heon
AU  - Shin MH
AUID- ORCID: 0000-0001-8207-6110
AD  - Department of Environmental Medical Biology and Institute of Tropical Medicine, 
      Yonsei University College of Medicine, Seoul, Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200918
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Immunologic Factors)
RN  - 0 (Leukotrienes)
RN  - 0 (Receptors, Leukotriene)
RN  - 0 (cysteinyl-leukotriene)
RN  - EC 1.6.3.- (CYBB protein, human)
RN  - EC 1.6.3.- (NADPH Oxidase 2)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Cell Degranulation
MH  - Cell Line
MH  - Cell Movement
MH  - Chemokine CCL2/*metabolism
MH  - Cysteine/*metabolism
MH  - Humans
MH  - Immunologic Factors/*metabolism
MH  - Leukotrienes/*metabolism
MH  - Mast Cells/metabolism/*physiology
MH  - NADPH Oxidase 2/metabolism
MH  - Receptors, Leukotriene/metabolism
MH  - Signal Transduction
MH  - Trichomonas vaginalis/*metabolism
OTO - NOTNLM
OT  - Trichomonas vaginalis
OT  - NADPH oxidase 2
OT  - cysteinyl leukotrienes
OT  - mast cells
OT  - monocyte chemoattractant protein-1
EDAT- 2020/09/04 06:00
MHDA- 2021/02/13 06:00
CRDT- 2020/09/04 06:00
PHST- 2020/06/22 00:00 [received]
PHST- 2020/08/22 00:00 [revised]
PHST- 2020/08/24 00:00 [accepted]
PHST- 2020/09/04 06:00 [pubmed]
PHST- 2021/02/13 06:00 [medline]
PHST- 2020/09/04 06:00 [entrez]
AID - 10.1111/pim.12789 [doi]
PST - ppublish
SO  - Parasite Immunol. 2020 Dec;42(12):e12789. doi: 10.1111/pim.12789. Epub 2020 Sep 
      18.