PMID- 32882929
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 21
IP  - 17
DP  - 2020 Sep 1
TI  - Cellular Signaling and Anti-Apoptotic Effects of Prolactin-Releasing Peptide and 
      Its Analog on SH-SY5Y Cells.
LID - 10.3390/ijms21176343 [doi]
LID - 6343
AB  - Prolactin-releasing peptide (PrRP), a natural ligand for the GPR10 receptor, is a 
      neuropeptide with anorexigenic and antidiabetic properties. Due to its role in 
      the regulation of food intake, PrRP is a potential drug for obesity treatment and 
      associated type 2 diabetes mellitus (T2DM). Recently, the neuroprotective effects 
      of lipidized PrRP analogs have been proven. In this study, we focused on the 
      molecular mechanisms of action of natural PrRP31 and its lipidized analog 
      palm(11)-PrRP31 in the human neuroblastoma cell line SH-SY5Y to describe their 
      cellular signaling and possible anti-apoptotic properties. PrRP31 significantly 
      upregulated the phosphoinositide-3 kinase-protein kinase B/Akt (PI3K-PKB/Akt) and 
      extracellular signal-regulated kinase/cAMP response element-binding protein 
      (ERK-CREB) signaling pathways that promote metabolic cell survival and growth. In 
      addition, we proved via protein kinase inhibitors that activation of signaling 
      pathways is mediated specifically by PrRP31 and its palmitoylated analog. 
      Furthermore, the potential neuroprotective properties were studied through 
      activation of anti-apoptotic pathways of PrRP31 and palm(11)-PrRP31 using the 
      SH-SY5Y cell line and rat primary neuronal culture stressed with toxic 
      methylglyoxal (MG). The results indicate increased viability of the cells treated 
      with PrRP and palm(11)-PrRP31 and a reduced degree of apoptosis induced by MG, 
      suggesting their potential use in the treatment of neurological disorders.
FAU - Zmeskalova, Anna
AU  - Zmeskalova A
AD  - Institute of Organic Chemistry and Biochemistry, AS CR, Prague 16000, Czech 
      Republic.
AD  - First Faculty of Medicine, Charles University, Prague 12108, Czech Republic.
FAU - Popelova, Andrea
AU  - Popelova A
AD  - Institute of Organic Chemistry and Biochemistry, AS CR, Prague 16000, Czech 
      Republic.
FAU - Exnerova, Aneta
AU  - Exnerova A
AD  - Institute of Organic Chemistry and Biochemistry, AS CR, Prague 16000, Czech 
      Republic.
FAU - Zelezna, Blanka
AU  - Zelezna B
AD  - Institute of Organic Chemistry and Biochemistry, AS CR, Prague 16000, Czech 
      Republic.
FAU - Kunes, Jaroslav
AU  - Kunes J
AD  - Institute of Organic Chemistry and Biochemistry, AS CR, Prague 16000, Czech 
      Republic.
AD  - Institute of Physiology, AS CR, Prague 14200, Czech Republic.
FAU - Maletinska, Lenka
AU  - Maletinska L
AD  - Institute of Organic Chemistry and Biochemistry, AS CR, Prague 16000, Czech 
      Republic.
LA  - eng
GR  - 20-00546S/Grantova Agentura Ceske Republiky/
GR  - RVO:61388963/Akademie Ved Ceske Republiky/
PT  - Journal Article
DEP - 20200901
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Prolactin-Releasing Hormone)
SB  - IM
MH  - *Apoptosis
MH  - Apoptosis Regulatory Proteins/*metabolism
MH  - Humans
MH  - Neuroblastoma/*drug therapy/metabolism/pathology
MH  - Neuropeptides/chemistry/*pharmacology
MH  - Neuroprotective Agents/chemistry/*pharmacology
MH  - Prolactin-Releasing Hormone/chemistry/*pharmacology
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
PMC - PMC7503370
OTO - NOTNLM
OT  - SH-SY5Y
OT  - cellular signaling
OT  - inhibitors
OT  - methylglyoxal
OT  - neuroprotection
OT  - primary neuronal culture
OT  - prolactin-releasing peptide
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/09/05 06:00
MHDA- 2021/03/30 06:00
PMCR- 2020/09/01
CRDT- 2020/09/05 06:00
PHST- 2020/07/10 00:00 [received]
PHST- 2020/08/27 00:00 [revised]
PHST- 2020/08/29 00:00 [accepted]
PHST- 2020/09/05 06:00 [entrez]
PHST- 2020/09/05 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/09/01 00:00 [pmc-release]
AID - ijms21176343 [pii]
AID - ijms-21-06343 [pii]
AID - 10.3390/ijms21176343 [doi]
PST - epublish
SO  - Int J Mol Sci. 2020 Sep 1;21(17):6343. doi: 10.3390/ijms21176343.