PMID- 32885155
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220416
IS  - 2589-0514 (Electronic)
IS  - 2095-882X (Print)
IS  - 2095-882X (Linking)
VI  - 6
IP  - 3
DP  - 2020 Sep
TI  - High-fat diet-induced adipose tissue expansion occurs prior to insulin resistance 
      in C57BL/6J mice.
PG  - 198-207
LID - 10.1016/j.cdtm.2020.06.003 [doi]
AB  - BACKGROUND: To date, there is only scare evidence characterizing the temporal 
      features and progression of metabolic dysfunction in high-fat diet (HFD)-fed 
      obese mice. Hence, its specific pathogenesis remains unclear. METHODS: Sixty 
      6-week-old male C57BL/6J mice were randomly divided into HFD and control diet 
      (CD) groups and sacrificed at 1, 5, 9, 13, 17, and 21 weeks, respectively. At 
      weekly intervals, intraperitoneal glucose tolerance testing (IPGTT) and 
      intraperitoneal insulin tolerance testing (IPITT) were performed in both groups. 
      A detailed time course in HFD-fed mice was investigated by evaluating the 
      initiation of glucose homeostasis impairment, dyslipidemia, systemic insulin 
      sensitivity, monocyte chemoattractant protein-1 (MCP-1) levels, epididymal white 
      adipose tissue (eWAT) expansion, macrophage content changes, pro-inflammatory 
      (M1)/anti-inflammatory (M2) macrophage imbalance, lipid accumulation in the 
      liver, and beta-cell morphometry in the pancreas. RESULTS: In the HFD group, 
      progressive weight gain and impairments in glucose metabolism (elevated fasting 
      blood glucose and area under the curve (AUC) of IPGTT) were observed from the 3rd 
      week, and a significantly elevated AUC of IPITT was first detected after week 7 
      of HFD feeding. As for dyslipidemia, after 9 weeks of feeding, the low-density 
      lipoprotein cholesterol level and total cholesterol level in HFD group were 
      significantly higher than those in the CD group (all P < 0.05), whereas no 
      significant differences were shown in triglyceride level. Adipocyte size 
      increased significantly in the HFD group in the 1st week, a phenotypic switch in 
      eWAT from anti-inflammatory (M2) to pro-inflammatory (M1) macrophages was 
      observed in the 5th week, and the metabolic inflammation was distinct in eWAT in 
      the 9th week. Additionally, liver steatosis was considerably obvious at the 17th 
      week and pancreatic beta-cell morphometry did not change during 21 weeks of HFD 
      feeding. CONCLUSION: The eWAT expansion was detected early in HFD-induced obese 
      mice, which occurred prior to obvious insulin resistance.
CI  - (c) 2020 Chinese Medical Association. Production and hosting by Elsevier B.V. on 
      behalf of KeAi Communications Co., Ltd.
FAU - He, Ming-Qian
AU  - He MQ
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Wang, Jing-Ya
AU  - Wang JY
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Wang, Yue
AU  - Wang Y
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Sui, Jing
AU  - Sui J
AD  - Department of Endocrinology and International Medical Center, The First 
      Affiliated Hospital of Xi'an JiaoTong University, Xi'an, Shaanxi 710061, China.
FAU - Zhang, Meng
AU  - Zhang M
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Ding, Xi
AU  - Ding X
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Zhao, Yang
AU  - Zhao Y
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Chen, Zi-Yi
AU  - Chen ZY
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Ren, Xiao-Xiao
AU  - Ren XX
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
FAU - Shi, Bing-Yin
AU  - Shi BY
AD  - Department of Endocrinology, The First Affiliated Hospital of Xi'an JiaoTong 
      University, Xi'an, Shaanxi 710061, China.
LA  - eng
PT  - Journal Article
DEP - 20200718
PL  - United States
TA  - Chronic Dis Transl Med
JT  - Chronic diseases and translational medicine
JID - 101679934
PMC - PMC7451745
OTO - NOTNLM
OT  - High-fat diet
OT  - Metabolic dysfunction
OT  - Metabolic inflammation
OT  - Obesity
COIS- None.
EDAT- 2020/09/05 06:00
MHDA- 2020/09/05 06:01
PMCR- 2020/07/18
CRDT- 2020/09/05 06:00
PHST- 2019/11/09 00:00 [received]
PHST- 2020/09/05 06:00 [entrez]
PHST- 2020/09/05 06:00 [pubmed]
PHST- 2020/09/05 06:01 [medline]
PHST- 2020/07/18 00:00 [pmc-release]
AID - S2095-882X(20)30062-1 [pii]
AID - 10.1016/j.cdtm.2020.06.003 [doi]
PST - epublish
SO  - Chronic Dis Transl Med. 2020 Jul 18;6(3):198-207. doi: 
      10.1016/j.cdtm.2020.06.003. eCollection 2020 Sep.