PMID- 32886793
OWN - NLM
STAT- MEDLINE
DCOM- 20210713
LR  - 20210905
IS  - 1945-2403 (Electronic)
IS  - 0146-4760 (Print)
IS  - 0146-4760 (Linking)
VI  - 45
IP  - 6
DP  - 2021 Jul 10
TI  - Development of an Analytical Method for Quantitation of Deoxynivalenol by 
      UPLC-MS-MS: A Preliminary Assessment of Gestational and Lactational Transfer in 
      Rats.
PG  - 566-572
LID - 10.1093/jat/bkaa119 [doi]
AB  - Deoxynivalenol (DON) is the most widely distributed trichothecene mycotoxin in 
      grain-based foods and animal feed. Exposure to DON is widespread as it has been 
      detected in food sources from around the world. The objective of this work was to 
      develop a method to quantitate DON in biological matrices and apply it in a 
      preliminary assessment of gestational and lactational transfer of DON following 
      exposure of pregnant rats. The method used protein precipitation followed by 
      ultra-performance liquid chromatography-tandem mass spectrometry. The method was 
      evaluated in male Sprague Dawley rat plasma over the concentration range  approximately 2-1,000 
      ng/mL. The method was linear (r >/= 0.99), accurate (mean relative error </= +/-4.9%) 
      and precise (relative standard deviation </= 5.5%). The mean absolute recovery was 
      85.9%. The limit of detection was 0.35 ng/mL. The method was also evaluated in 
      gestational day (GD) 18 Hsd:Sprague Dawley(R)SD(R) dam plasma and fetal homogenate 
      (mean % relative error </= +/-16.9; % relative standard deviation </= 9.5). 
      Concentrations of DON in dam plasma stored at -80 degrees C for at least 29 days and in 
      fetal homogenate for at least 43 days were within 97.9 to 120% of Day 0 
      concentrations, demonstrating that DON is stable in these matrices. The method 
      was used to quantitate DON in rat maternal plasma, amniotic fluid, GD 18 fetuses 
      and postnatal day (PND) 4 pups following exposure of dams to 0 (control) and 1 
      mg/kg DON beginning on GD 6 and continuing through gestation and lactation for a 
      preliminary assessment of maternal transfer. In animals exposed to 1 mg/kg/day, 
      similar concentration of DON was found in GD 18 dam plasma and fetuses, 
      demonstrating significant gestational transfer. The concentration of DON in PND 4 
      dam plasma was similar to that in GD 18 dam plasma. However, DON was not detected 
      in PND 4 pup plasma above the limit of detection of the assay, demonstrating 
      absence of transfer of DON to pups via lactation.
CI  - (c) The Author(s) 2020. Published by Oxford University Press. All rights reserved. 
      For permissions, please e-mail: journals.permissions@oup.com.
FAU - Rehder Silinski, Melanie A
AU  - Rehder Silinski MA
AD  - RTI International, P.O. Box 12194, Research Triangle Park, NC 27709, USA.
FAU - Gilliam, Jennifer A
AU  - Gilliam JA
AD  - RTI International, P.O. Box 12194, Research Triangle Park, NC 27709, USA.
FAU - Fernando, Reshan A
AU  - Fernando RA
AD  - RTI International, P.O. Box 12194, Research Triangle Park, NC 27709, USA.
FAU - Robinson, Veronica G
AU  - Robinson VG
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
FAU - Germolec, Dori
AU  - Germolec D
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
FAU - Cunny, Helen
AU  - Cunny H
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
FAU - Huang, Madelyn C
AU  - Huang MC
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
FAU - Furr, Johnathan
AU  - Furr J
AD  - Southern Research, Birmingham, AL 35205, USA.
FAU - Waidyanatha, Suramya
AU  - Waidyanatha S
AD  - Division of the National Toxicology Program, NIEHS, P.O. Box 12233, Research 
      Triangle Park, NC 27709, USA.
LA  - eng
GR  - HHSN273201300010C/ES/NIEHS NIH HHS/United States
GR  - HHSN273201300010I/ES/NIEHS NIH HHS/United States
GR  - HHSN273201400022C/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PL  - England
TA  - J Anal Toxicol
JT  - Journal of analytical toxicology
JID - 7705085
RN  - 0 (Trichothecenes)
RN  - JT37HYP23V (deoxynivalenol)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid
MH  - Chromatography, Liquid
MH  - Female
MH  - *Lactation
MH  - Male
MH  - Pregnancy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - *Tandem Mass Spectrometry
MH  - Trichothecenes
PMC - PMC8272532
EDAT- 2020/09/05 06:00
MHDA- 2021/07/14 06:00
PMCR- 2021/09/04
CRDT- 2020/09/04 17:10
PHST- 2020/04/17 00:00 [received]
PHST- 2020/07/06 00:00 [revised]
PHST- 2020/08/31 00:00 [accepted]
PHST- 2020/09/05 06:00 [pubmed]
PHST- 2021/07/14 06:00 [medline]
PHST- 2020/09/04 17:10 [entrez]
PHST- 2021/09/04 00:00 [pmc-release]
AID - 5901576 [pii]
AID - bkaa119 [pii]
AID - 10.1093/jat/bkaa119 [doi]
PST - ppublish
SO  - J Anal Toxicol. 2021 Jul 10;45(6):566-572. doi: 10.1093/jat/bkaa119.