PMID- 32887050
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 219
DP  - 2020 Nov 1
TI  - Online high-efficient specific detection of zearalenone in rice by using 
      high-loading aptamer affinity hydrophilic monolithic column coupled with HPLC.
PG  - 121309
LID - S0039-9140(20)30600-7 [pii]
LID - 10.1016/j.talanta.2020.121309 [doi]
AB  - Via the facile photopolymerization and thiol-ene click chemistry, a hydrophilic 
      polyhedral oligomeric silsesquioxane (POSS)-containing affinity monolithic column 
      with a high load of aptamers and fast preparation was presented, and successfully 
      applied to the efficient specific recognition and sensitive detection of 
      zearalenone (ZEN) by coupling with HPLC. Optimization of polymerization reaction 
      and polymer recipe were studied, and characteristics such as structural 
      morphology, affinity performance and specificity to ZEN were evaluated. A highly 
      hydrophilic POSS-based aptamer affinity monolith was rapidly prepared in 1.1 h, 
      and a high capacity of aptamer reached 2772.61 pmol/muL that was much higher than 
      that of the current POSS-based aptamer affinity monoliths. By this way, the 
      hydrophilic nature and aptamer coverage indensity in POSS monoliths were well 
      improved. The mixed-mode mechanism including aptamer affinity recognition and 
      hydrophilic interaction were employed for the specific analysis of ZEN. Applied 
      to the monitoring of ZEN, a good selective recognition and sensitive detection 
      were obtained with the detection limit (LOD) of 0.02 ng/mL. The stability and 
      reproducibility were acceptable and the intra-day, inter-day and column-to-column 
      relative standard deviations (RSDs) of the ZEN recovery were gained in the range 
      from 2.28% to 5.32%, respectively. Satisfactory recoveries of ZEN in rice samples 
      were realized ad 98.6 +/- 2.8%-100.8 +/- 2.0%. It might provide a preferable access 
      to online specific analysis of ZEN via the hydrophilic POSS-based affinity 
      monolith with a large capacity of aptamers and a high preparation efficiency.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Xu, Jinhua
AU  - Xu J
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, China.
FAU - Liu, Ting
AU  - Liu T
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, China.
FAU - Chi, Jinxin
AU  - Chi J
AD  - Xiamen Huaxia University, Xiamen, 361024, China.
FAU - Zhang, Wen
AU  - Zhang W
AD  - Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and 
      Chemotherapy, Fuzhou University, Fuzhou, 350108, China.
FAU - Lin, Chenchen
AU  - Lin C
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, China.
FAU - Lin, Xucong
AU  - Lin X
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, China. Electronic address: xulin@fzu.edu.cn.
FAU - Xie, Zenghong
AU  - Xie Z
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, China. Electronic address: zhxie@fzu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200627
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 5W827M159J (Zearalenone)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Hydrophobic and Hydrophilic Interactions
MH  - *Oryza
MH  - Reproducibility of Results
MH  - *Zearalenone/analysis
OTO - NOTNLM
OT  - Aptamer affinity monolith
OT  - Hydrophilic
OT  - Online
OT  - Specific detection
OT  - Zearalenone
EDAT- 2020/09/06 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/09/05 01:02
PHST- 2020/02/27 00:00 [received]
PHST- 2020/06/13 00:00 [revised]
PHST- 2020/06/17 00:00 [accepted]
PHST- 2020/09/05 01:02 [entrez]
PHST- 2020/09/06 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
AID - S0039-9140(20)30600-7 [pii]
AID - 10.1016/j.talanta.2020.121309 [doi]
PST - ppublish
SO  - Talanta. 2020 Nov 1;219:121309. doi: 10.1016/j.talanta.2020.121309. Epub 2020 Jun 
      27.