PMID- 32887256
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201023
IS  - 2309-608X (Electronic)
IS  - 2309-608X (Linking)
VI  - 6
IP  - 3
DP  - 2020 Sep 2
TI  - Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric 
      Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model.
LID - 10.3390/jof6030160 [doi]
LID - 160
AB  - Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the 
      dimorphic fungal species of Paracoccidioides, which mainly affects the lungs. 
      Modern strategies for the treatment and/or prevention of PCM are based on a 
      Th1-type immune response, which is important for controlling the disease. One of 
      the most studied candidates for a vaccine is the P10 peptide, derived from the 43 
      kDa glycoprotein of Paracoccidioides brasiliensis. In order to improve its immune 
      modulatory effect, the P10 peptide was associated with a chitosan-conjugated 
      nanoparticle. The nanoparticles presented 220 nm medium size, poly dispersion 
      index (PDI) below 0.5, zeta potential of +20 mV and encapsulation efficiency 
      around 90%. The nanoparticles' non-toxicity was verified by hemolytic test and 
      cell viability using murine macrophages. The nanoparticles were stable and 
      presented physicochemical characteristics desirable for biological applications, 
      reducing the fungal load and the usual standard concentration of the peptide from 
      4 to 20 times.
FAU - Rodrigues Dos Santos Junior, Samuel
AU  - Rodrigues Dos Santos Junior S
AUID- ORCID: 0000-0001-5564-3538
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP 05508-000, Brazil.
FAU - Kelley Lopes da Silva, Francenya
AU  - Kelley Lopes da Silva F
AD  - Laboratorio de Nano & Biotecnologia, Departamento de Biotecnologia, Instituto de 
      Patologia Tropical e Saude Publica, Universidade Federal de Goias, Goiania, GO 
      74605-050, Brazil.
FAU - Santos Dias, Lucas
AU  - Santos Dias L
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP 05508-000, Brazil.
FAU - Oliveira Souza, Ana Camila
AU  - Oliveira Souza AC
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP 05508-000, Brazil.
FAU - Valdemir de Araujo, Marcelo
AU  - Valdemir de Araujo M
AUID- ORCID: 0000-0001-9695-3225
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP 05508-000, Brazil.
FAU - Buffoni Roque da Silva, Leandro
AU  - Buffoni Roque da Silva L
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP 05508-000, Brazil.
FAU - Travassos, Luiz R
AU  - Travassos LR
AD  - Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal 
      de Sao Paulo, Sao Paulo, SP 04023-062, Brazil.
FAU - Correa Amaral, Andre
AU  - Correa Amaral A
AD  - Laboratorio de Nano & Biotecnologia, Departamento de Biotecnologia, Instituto de 
      Patologia Tropical e Saude Publica, Universidade Federal de Goias, Goiania, GO 
      74605-050, Brazil.
FAU - P Taborda, Carlos
AU  - P Taborda C
AUID- ORCID: 0000-0001-9928-9809
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP 05508-000, Brazil.
AD  - Departamento de Dermatologia, Instituto de Medicina Tropical de Sao Paulo, 
      Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP 05403-000, 
      Brazil.
LA  - eng
GR  - 2016/08730-6/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/
GR  - 420480/2018-8 and 134424/2016-6/Conselho Nacional de Desenvolvimento Cientifico e 
      Tecnologico/
PT  - Journal Article
DEP - 20200902
PL  - Switzerland
TA  - J Fungi (Basel)
JT  - Journal of fungi (Basel, Switzerland)
JID - 101671827
PMC - PMC7560165
OTO - NOTNLM
OT  - P10 peptide
OT  - antifungal therapy
OT  - nanovaccine
OT  - paracoccidioidomycosis
OT  - polymeric nanoparticles
COIS- There are no conflicts of interest to declare.
EDAT- 2020/09/06 06:00
MHDA- 2020/09/06 06:01
PMCR- 2020/09/02
CRDT- 2020/09/05 01:03
PHST- 2020/08/13 00:00 [received]
PHST- 2020/08/22 00:00 [revised]
PHST- 2020/08/25 00:00 [accepted]
PHST- 2020/09/05 01:03 [entrez]
PHST- 2020/09/06 06:00 [pubmed]
PHST- 2020/09/06 06:01 [medline]
PHST- 2020/09/02 00:00 [pmc-release]
AID - jof6030160 [pii]
AID - jof-06-00160 [pii]
AID - 10.3390/jof6030160 [doi]
PST - epublish
SO  - J Fungi (Basel). 2020 Sep 2;6(3):160. doi: 10.3390/jof6030160.